| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DIDO1 |
| Uniprot No | Q9BTC0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-544aa |
| 氨基酸序列 | MDDKGDPSNEEAPKAIKPTSKEFRKTWGFRRTTIAKREGAGDAEADPLEP PPPQQQLGLSLRRSGRQPKRTERVEQFLTIARRRGRRSMPVSLEDSGEPT SCPATDAETASEGSVESASETRSGPQSASTAVKERPASSEKVKGGDDHDD TSDSDSDGLTLKELQNRLRRKREQEPTERPLKGIQSRLRKKRREEGPAET VGSEASDTVEGVLPSKQEPENDQGVVSQAGKDDRESKLEGKAAQDIKDEE PGDLGRPKPECEGYDPNALYCICRQPHNNRFMICCDRCEEWFHGDCVGIS EARGRLLERNGEDYICPNCTILQVQDETHSETADQQEAKWRPGDADGTDC TSIGTIEQKSSEDQGIKGRIEKAANPSGKKKLKIFQPVIEAPGASKCIGP GCCHVAQPDSVYCSNDCILKHAAATMKFLSSGKEQKPKPKEKMKMKPEKP SLPKCGAQAGIKISSVHKRPAPEKKETTVKKAVVVPARSEALGKEAACES STPSWASDHNYNAVKPEKTAAPSPSLLYKCSGKYLYSLHPSLIA |
| 预测分子量 | 86 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"Structural and Functional Characterization of Recombinant DIDO1 Splice Variants"**
- *Authors: Müller et al.*
- 摘要：通过大肠杆菌表达系统纯化DIDO1的两种重组剪接变体，分析其磷酸化修饰及与染色质结合能力的差异，揭示剪接对功能的影响。
2. **"Expression and Purification of the DIDO1 N-terminal Domain for Crystallization Studies"**
- *Authors: Lee & Park*
- 摘要：利用昆虫细胞系统表达DIDO1的N端结构域，优化纯化步骤并获得晶体，为后续结构解析及功能机制研究奠定基础。
3. **"DIDO1 Recombinant Protein as a Novel Biomarker in Leukemia"**
- *Authors: Chen et al.*
- 摘要：构建His标签的DIDO1重组蛋白，验证其在白血病细胞中的异常表达，并探索其作为预后标志物的潜力。
4. **"In Vitro Interaction Assays of DIDO1 with Chromatin Remodeling Complexes"**
- *Authors: Rossi et al.*
- 摘要：通过重组DIDO1蛋白与染色质重塑复合物的体外互作实验，证明其通过PH结构域介导与组蛋白修饰酶的协同作用。
(注：以上文献信息为示例性概括，实际文献需通过PubMed或Web of Science等平台检索确认。)
**Background of DIDO1 Recombinant Protein**
DIDO1 (Death Inducer-Obliterator 1) is a nuclear protein encoded by the *DIDO1* gene, initially identified for its role in apoptosis and hematopoietic differentiation. It belongs to the DIDO protein family, which includes splice variants with diverse cellular functions. Structurally, DIDO1 contains multiple functional domains, such as AT-hook motifs for DNA binding, nuclear localization signals (NLS), and a conserved C-terminal SPOC (Spliceosome-associated Protein C-terminal) domain implicated in transcriptional regulation and RNA splicing.
As a regulator of cell cycle progression, DIDO1 is critical during mitosis, particularly in maintaining chromosomal stability and ensuring proper sister chromatid segregation. Studies suggest its involvement in the spindle assembly checkpoint (SAC), where it interacts with components like Aurora B kinase to monitor mitotic fidelity. Dysregulation of DIDO1 has been linked to genomic instability, a hallmark of cancer, and its overexpression or truncation is observed in certain malignancies.
Recombinant DIDO1 protein is engineered using expression systems like *E. coli* or mammalian cells, enabling large-scale production for functional studies. Purification techniques, such as affinity chromatography with His-tags or GST-tags, ensure high specificity. Researchers utilize this recombinant protein to investigate DIDO1's molecular interactions, post-translational modifications (e.g., phosphorylation), and its role in signaling pathways. Additionally, it serves as an antigen for antibody development or a tool in drug screening assays targeting mitotic regulators.
Current research focuses on elucidating DIDO1's dual role in apoptosis and mitosis, its potential as a cancer biomarker, and its interplay with epigenetic modifiers. The recombinant protein remains pivotal in unraveling its mechanistic contributions to cell fate decisions and disease pathogenesis.
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