Recombinant Human PTI protein

中文名： 胎盘凝血酶抑制因子(PTI)重组蛋白
别名： PTI；PI6；PTI；Serpin B6

货号: PA1000-9200

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>90%SDS-PAGE.
种属	Human
靶点	PTI
Uniprot No	P35237
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	1-376aa
氨基酸序列	MDVLAEANGT FALNLLKTLG KDNSKNVFFS PMSMSCALAM VYMGAKGNTA AQMAQILSFN KSGGGGDIHQ GFQSLLTEVN KTGTQYLLRM ANRLFGEKSC DFLSSFRDSC QKFYQAEMEE LDFISAVEKS RKHINTWVAE KTEGKIAELL SPGSVDPLTR LVLVNAVYFR GNWDEQFDKE NTEERLFKVS KNEEKPVQMM FKQSTFKKTY IGEIFTQILV LPYVGKELNM IIMLPDETTD LRTVEKELTY EKFVEWTRLD MMDEEEVEVS LPRFKLEESY DMESVLRNLG MTDAFELGKA DFSGMSQTDL SLSKVVHKSF VEVNEEGTEA AAATAAIMMM RCARFVPRFC ADHPFLFFIQ HSKTNGILFC GRFSSP
预测分子量	kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是3条关于PTI(Pattern-Triggered Immunity，模式触发免疫)相关重组蛋白研究的参考文献示例：

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1. **《Reconstitution of a plant receptor-like kinase signaling platform in vitro》**

- **作者**: Wang J. et al.

- **摘要**: 该研究通过重组表达拟南芥的FLS2和BAK1受体蛋白，体外重建了植物PTI信号复合体，揭示了其识别细菌鞭毛蛋白(flg22)后激活的分子机制。

2. **《The EF-Tu receptor EFR restricts Agrobacterium-mediated plant transformation》**

- **作者**: Lacombe S. et al.

- **摘要**: 利用重组表达的EFR受体蛋白，证明其特异性识别细菌延伸因子EF-Tu，触发PTI反应，并抑制农杆菌介导的植物遗传转化。

3. **《Ligand-induced kinase activity of the Arabidopsis pattern recognition receptor FLS2》**

- **作者**: Schulze B. et al.

- **摘要**: 通过重组FLS2蛋白的体外激酶活性实验，揭示了该受体在结合flg22后发生自磷酸化，进而激活下游免疫信号通路。

4. **《Recombinant CERK1 extracellular domain inhibits chitin signaling in Arabidopsis》**

- **作者**: Liu B. et al.

- **摘要**: 研究通过表达几丁质受体CERK1的胞外域重组蛋白，证明其竞争性结合几丁质，阻断PTI信号传导，为病原体逃逸植物免疫提供新见解。

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以上文献均围绕植物PTI中关键受体蛋白的重组表达与功能验证展开，涵盖受体-配体互作、信号激活及免疫调控机制。实际引用时建议核查具体期刊与年份信息。

背景信息

**Background of PTI Recombinant Proteins**

Recombinant proteins, including those related to pathogen-triggered immunity (PTI), are engineered through genetic modification to express specific proteins in host organisms like bacteria, yeast, or mammalian cells. PTI refers to a plant's innate immune response activated upon detection of conserved microbial patterns (PAMPs) by cell-surface receptors. Proteins involved in PTI signaling, such as receptor-like kinases (RLKs), MAP kinases, or transcription factors, are of significant interest for their roles in enhancing disease resistance in crops.

The development of PTI-associated recombinant proteins emerged from the need to study plant immune mechanisms and engineer disease-resistant crops. By cloning genes encoding PTI-related proteins into expression systems, researchers produce purified proteins for functional studies, structural analysis, or biotechnological applications. For example, recombinant flagellin (a bacterial PAMP) or its receptor FLS2 have been used to dissect early immune signaling events.

Applications of PTI recombinant proteins span agriculture and biotechnology. Engineered proteins may serve as bio-stimulants to prime plant immunity or as tools to screen for novel antimicrobial agents. In crop breeding, recombinant proteins aid in identifying genetic variants with enhanced PTI responses. Additionally, they contribute to sustainable agriculture by reducing reliance on chemical pesticides.

Challenges include maintaining protein stability and activity post-purification, as well as scaling production cost-effectively. Advances in synthetic biology and CRISPR-based gene editing are refining recombinant protein design and host systems. Future directions may focus on optimizing PTI protein delivery methods, such as nanoparticle carriers, or engineering crops with tailored immune receptors. Overall, PTI recombinant proteins represent a bridge between fundamental plant science and practical solutions for global food security.