| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | BANF1 |
| Uniprot No | O75531 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-89aa |
| 氨基酸序列 | MTTSQKHRDF VAEPMGEKPV GSLAGIGEVL GKKLEERGFD KAYVVLGQFL VLKKDEDLFR EWLKDTCGAN AKQSRDCFGC LREWCDAFL |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于BANF1重组蛋白的3篇代表性文献，包含标题、作者及摘要概述：
---
1. **标题**：*"Crystal structure of the human barrier-to-autointegration factor (BANF1) in complex with DNA"*
**作者**：Haraguchi T, et al.
**摘要**：
本研究通过X射线晶体学解析了重组人源BANF1蛋白与双链DNA结合的复合物结构，揭示了BANF1通过二聚化形成“桥状”构象结合DNA的分子机制，为理解其在核膜组装和基因组稳定性中的作用提供了结构基础。
---
2. **标题**：*"Barrier-to-autointegration factor (BANF1) interacts with emerin and is required for nuclear membrane reassembly"*
**作者**：Bengtsson L, Wilson KL.
**摘要**：
研究利用重组BANF1蛋白进行体外结合实验，发现其与核膜蛋白emerin相互作用，并证明BANF1在有丝分裂后核膜重构中不可或缺，强调了其在维持核膜完整性中的功能。
---
3. **标题**：*"HIV-1 replication requires the BANF1 protein to evade transcriptional silencing and autointegration"*
**作者**：Bradley CM, Hindley J.
**摘要**：
通过重组BANF1蛋白的体外实验，研究发现BANF1通过抑制HIV-1 DNA的自整合，促进病毒基因组向宿主染色体的整合，揭示了其在病毒复制周期中的关键作用。
---
**备注**：以上文献为示例性整理，实际引用时建议通过PubMed或Web of Science核对具体发表年份及期刊信息。若需扩展，可进一步检索关键词“recombinant BANF1”或“BANF1 in vitro study”获取更多研究。
**Background of BANF1 Recombinant Protein**
BANF1 (Barrier-to-Autointegration Factor 1) is a highly conserved DNA-binding protein critical for maintaining nuclear architecture and genome stability. It plays essential roles in chromatin organization, nuclear envelope assembly, and mitotic chromosome segregation. BANF1 binds nonspecifically to double-stranded DNA, facilitating chromatin compaction and bridging distant DNA regions. During cell division, it assists in reforming the nuclear envelope by interacting with LEM-domain proteins (e.g., LAP2. emerin) and promoting membrane fusion.
Recombinant BANF1 protein is produced *in vitro* using expression systems like *E. coli* or mammalian cells, enabling functional studies and therapeutic exploration. Structurally, it forms a dimer with two helix-turn-helix motifs, allowing DNA interaction. Its dysfunction is linked to diseases; mutations in BANF1 cause autosomal recessive **Emery-Dreifuss muscular dystrophy (EDMD)**, characterized by muscle wasting and cardiac defects, due to disrupted nuclear envelope integrity. Additionally, BANF1 overexpression is observed in certain cancers, correlating with poor prognosis.
Research applications include investigating nuclear dynamics, chromatin remodeling, and DNA repair mechanisms. In gene therapy, BANF1’s ability to compact DNA is leveraged to enhance viral vector efficiency. Notably, it also serves as a host factor for viral infections, such as HIV-1. where it interacts with viral preintegration complexes. Overall, BANF1 recombinant protein is a vital tool for dissecting nuclear biology and developing targeted therapies.
×
