| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ZG16B |
| Uniprot No | Q96DA0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 53-208aa |
| 氨基酸序列 | GKMYGPGGGKYFSTTEDYDHEITGLRVSVGLLLVKSVQVKLGDSWDVKLGALGGNTQEVTLQPGEYITKVFVAFQAFLRGMVMYTSKDRYFYFGKLDGQISSAYPSQEGQVLVGIYGQYQLLGIKSIGFEWNYPLEEPTTEPPVNLTYSANSPVGR |
| 预测分子量 | 20.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ZG16B重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**: *Expression and Functional Characterization of Recombinant ZG16B in Gastrointestinal Mucosal Immunity*
**作者**: Smith A, et al.
**摘要**: 研究通过大肠杆菌系统成功表达重组ZG16B蛋白，验证其与肠道黏蛋白的结合能力，并发现其通过凝集素结构域抑制病原菌黏附，提示其在黏膜免疫中的作用。
2. **文献名称**: *Structural Insights into ZG16B’s Role in Glycan Recognition via X-ray Crystallography*
**作者**: Tanaka K, et al.
**摘要**: 解析了重组ZG16B蛋白的晶体结构，揭示其独特的β-三叶草折叠模式及与多糖结合的关键氨基酸残基，为理解其与肠道菌群互作机制提供结构基础。
3. **文献名称**: *ZG16B Recombinant Protein Attenuates Colitis in Murine Models by Modulating Gut Microbiota*
**作者**: Chen L, et al.
**摘要**: 在小鼠结肠炎模型中，重组ZG16B通过调控肠道菌群丰度(如增加乳杆菌、抑制肠杆菌)，减少炎症因子表达，表明其治疗炎症性肠病的潜在价值。
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注：上述文献为示例性质，实际引用需以真实发表论文为准。建议通过PubMed或Google Scholar以关键词“ZG16B recombinant”检索最新研究。
ZG16B (Zymogen Granule Protein 16B) is a small, secreted protein encoded by the ZG16B gene, primarily expressed in the gastrointestinal tract, pancreas, and mucus-secreting cells. It belongs to the Jacalins superfamily of lectins, characterized by carbohydrate-binding domains. Structurally, ZG16B contains a conserved carbohydrate recognition domain (CRD) that facilitates interactions with glycans, particularly mannose-rich structures, suggesting roles in microbial recognition, mucosal immunity, or glycoprotein trafficking. Studies link ZG16B to the organization of zymogen granules in pancreatic acinar cells and the maintenance of intestinal mucus barrier integrity by regulating mucin secretion or cross-linking.
Recombinant ZG16B protein is produced using expression systems like Escherichia coli or mammalian cells, enabling functional studies without contamination from native biological matrices. Its production involves cloning the ZG16B gene into expression vectors, followed by purification via affinity chromatography. Researchers employ the recombinant protein to investigate its carbohydrate-binding specificity, interactions with gut microbiota, and potential immunomodulatory functions. Altered ZG16B expression has been observed in inflammatory bowel disease (IBD), colorectal cancer, and pancreatitis, positioning it as a biomarker candidate or therapeutic target. Recent studies also explore its role in autophagy and cellular stress responses. However, its precise molecular mechanisms remain partially understood, driving ongoing research into its physiological and pathological relevance. Recombinant ZG16B serves as a critical tool for elucidating its biological significance and translational applications in gastrointestinal disorders.
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