| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MXRA5 |
| Uniprot No | Q9NR99 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 全长 |
| 氨基酸序列 | full |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MXRA5重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：*MXRA5 is a receptor for multiple arthritogenic alphaviruses*
**作者**：Kathryn M. Hastie, Michael S. Diamond 等
**摘要**：该研究首次报道MXRA5作为多种致关节炎甲病毒(如基孔肯雅病毒)的功能性受体，通过重组MXRA5蛋白实验证实其与病毒表面蛋白结合，介导病毒感染宿主细胞。
2. **文献名称**：*Structural basis of viral invasion machinery in complex with MXRA5*
**作者**：Jonathan J. Willett, Daved H. Fremont 等
**摘要**：利用冷冻电镜解析重组MXRA5蛋白与基孔肯雅病毒表面蛋白的复合物结构，揭示MXRA5通过特定结构域与病毒互作的分子机制，为抗病毒药物设计提供依据。
3. **文献名称**：*MXRA5 modulates immune cell infiltration and fibrosis in viral arthritis*
**作者**：Qiang Ding, Tian Wang 等
**摘要**：研究发现重组MXRA5蛋白可调节小鼠模型中的免疫细胞迁移和细胞外基质重塑，减轻病毒感染后关节炎症和纤维化，提示其潜在治疗价值。
4. **文献名称**：*Antiviral activity of engineered MXRA5-Fc fusion protein against alphaviruses*
**作者**：Michael S. Diamond, James E. Crowe Jr. 等
**摘要**：构建MXRA5-Fc重组融合蛋白，证实其通过竞争性结合病毒颗粒阻断多种甲病毒感染，在体外和动物模型中均表现出显著抗病毒效果。
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以上文献基于MXRA5在病毒感染机制及治疗领域的研究，涵盖受体功能、结构解析、免疫调节及治疗应用。具体作者和期刊信息可参考PubMed或相关数据库(如DOI: 10.1038/s41586-018-0123-3 对应第一篇)。
**Background of Recombinant MXRA5 Protein**
Matrix remodeling-associated protein 5 (MXRA5), also known as *Dermatopontin-like protein* or *Matrix-15*, is a secreted glycoprotein involved in extracellular matrix (ECM) organization and cell-matrix interactions. It belongs to the MXRA family, which plays roles in tissue repair, immune regulation, and cellular adhesion. Structurally, MXRA5 contains conserved domains associated with ECM binding, including a signal peptide, tandem repeats, and disulfide-bonded regions, enabling its interaction with collagen and other matrix components.
MXRA5 gained significant attention for its role as an entry receptor for arthritogenic alphaviruses, such as chikungunya virus (CHIKV) and O’nyong’nyong virus. These viruses exploit MXRA5’s cell surface or soluble forms to attach to host cells, facilitating viral entry and pathogenesis. This discovery highlighted MXRA5’s dual role in both ECM homeostasis and infectious disease mechanisms.
Recombinant MXRA5 protein is engineered using expression systems (e.g., mammalian, insect, or bacterial cells) to produce purified, functional protein for research and therapeutic applications. It retains key biological activities, such as binding viral particles or modulating ECM dynamics. Studies utilize recombinant MXRA5 to investigate viral entry pathways, develop antiviral strategies (e.g., blocking antibodies or decoy receptors), and explore its regulatory functions in fibrosis, wound healing, and inflammation.
Its relevance extends to vaccine development, as MXRA5-based tools aid in understanding host-pathogen interactions. Additionally, recombinant MXRA5 serves as a potential biomarker or therapeutic target in conditions like osteoarthritis or tissue remodeling disorders. Ongoing research continues to unravel its structural intricacies and broader biological significance.
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