| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KCC4 |
| Uniprot No | Q16566 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-473aa |
| 氨基酸序列 | MLKVTVPSCSASSCSSVTASAAPGTASLVPDYWIDGSNRDALSDFFEVESELGRGATSIVYRCKQKGTQKPYALKVLKKTVDKKIVRTEIGVLLRLSHPNIIKLKEIFETPTEISLVLELVTGGELFDRIVEKGYYSERDAADAVKQILEAVAYLHENGIVHRDLKPENLLYATPAPDAPLKIADFGLSKIVEHQVLMKTVCGTPGYCAPEILRGCAYGPEVDMWSVGIITYILLCGFEPFYDERGDQFMFRRILNCEYYFISPWWDEVSLNAKDLVRKLIVLDPKKRLTTFQALQHPWVTGKAANFVHMDTAQKKLQEFNARRKLKAAVKAVVASSRLGSASSSHGSIQESHKASRDPSPIQDGNEDMKAIPEGEKIQGDGAQAAVKGAQAELMKVQALEKVKGADINAEEAPKMVPKAVEDGIKVADLELEEGLAEEKLKTVEEAAAPREGQGSSAVGFEVPQQDVILPEY |
| 预测分子量 | 51,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KCC4重组蛋白的参考文献示例(注：部分文献信息为假设性概括，实际引用需核实具体来源)：
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1. **文献名称**：*Molecular Cloning and Functional Characterization of KCC4: A Potassium-Chloride Cotransporter Expressed in Kidney and Inner Ear*
**作者**：Pearson, M.M., et al.
**摘要**：本研究首次报道了KCC4(SLC12A7)重组蛋白在HEK293细胞中的异源表达，证实其具有依赖于钾和氯离子的转运活性，并揭示其在肾脏髓质和内耳中的生理作用，为后续研究离子稳态机制奠定基础。
2. **文献名称**：*Structural Determinants of KCC4 Transport Activity Revealed by Recombinant Protein Mutagenesis*
**作者**：Casula, S., et al.
**摘要**：通过重组KCC4蛋白的体外功能分析，作者鉴定了跨膜结构域中关键氨基酸残基对离子转运活性的影响，发现特定突变可显著降低其氯离子亲和力，提示结构-功能关系在疾病相关突变中的作用。
3. **文献名称**：*KCC4 Recombinant Protein Rescue of Auditory Dysfunction in a Mouse Model*
**作者**：Uvarov, P., et al.
**摘要**：利用重组KCC4蛋白在小鼠模型中进行功能补偿实验，证明KCC4缺陷导致的内耳毛细胞离子失衡可通过外源性蛋白部分恢复，为遗传性耳聋治疗提供潜在策略。
4. **文献名称**：*High-Throughput Screening of KCC4 Modulators Using Fluorescence-Based Recombinant Assays*
**作者**：Delpire, E., et al.
**摘要**：开发了一种基于重组KCC4蛋白的荧光标记高通量筛选平台，用于快速鉴定调节其活性的小分子化合物，为开发针对神经系统或肾脏疾病的药物提供新方法。
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**注意**：以上文献为示例性内容，实际研究中请通过学术数据库(如PubMed、Web of Science)检索具体文献并核实信息。
KCC4. or potassium-chloride cotransporter 4. is a member of the solute carrier 12 (SLC12A) family of electroneutral cation-coupled chloride transporters. Encoded by the SLC12A7 gene in humans, KCC4 plays a critical role in maintaining ionic homeostasis by mediating the coupled extrusion of potassium (K⁺) and chloride (Cl⁻) ions across cell membranes. Unlike its closely related isoforms (e.g., KCC1. KCC2. and KCC3), KCC4 exhibits distinct tissue-specific expression patterns, with high abundance in the kidney, inner ear, and certain regions of the central nervous system. Its function is tightly linked to cell volume regulation, transepithelial ion transport, and the modulation of intracellular chloride concentration, which influences processes such as neuronal excitability and osmotic balance.
Structurally, KCC4 comprises 12 transmembrane domains with intracellular N- and C-termini, a hallmark of SLC12A proteins. Its activity is regulated by phosphorylation-dephosphorylation mechanisms, osmotic stress, and cellular pH. Dysregulation of KCC4 has been implicated in pathologies including renal tubular acidosis, sensorineural hearing loss, and neurodegenerative disorders. For instance, mutations in SLC12A7 are associated with autosomal recessive deafness and renal dysfunction in both humans and animal models, underscoring its physiological significance.
Recombinant KCC4 proteins are engineered using heterologous expression systems (e.g., HEK293 or CHO cells) to study its biophysical properties, regulatory pathways, and interactions with therapeutic agents. These proteins are pivotal in drug discovery for disorders linked to ion transport defects. Purified recombinant KCC4 retains functional activity in vitro, enabling high-throughput screening of modulators and structural studies to elucidate transport mechanisms. Its application extends to developing targeted therapies for conditions like hypertension, epilepsy, and neuropathic pain, where chloride homeostasis is disrupted. Overall, KCC4 recombinant proteins serve as indispensable tools for advancing both basic research and clinical innovation.
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