| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CX26 |
| Uniprot No | P29033 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-226aa |
| 氨基酸序列 | MDWGTLQTILGGVNKHSTSIGKIWLTVLFIFRIMILVVAAKEVWGDEQAD FVCNTLQPGCKNVCYDHYFPISHIRLWALQLIFVSTPALLVAMHVAYRRH EKKRKFIKGEIKSEFKDIEEIKTQKVRIEGSLWWTYTSSIFFRVIFEAAF MYVFYVMYDGFSMQRLVKCNAWPCPNTVDCFVSRPTEKTVFTVFMIAVSG ICILLNVTELCYLLIRYCSGKSKKPV |
| 预测分子量 | 51 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CX26(连接蛋白26)重组蛋白的3篇代表性文献摘要整理：
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1. **文献名称**：*Crystal structure of human connexin 26 gap junction channel*
**作者**：Maeda S. et al. (2009)
**摘要**：该研究首次解析了人源CX26重组蛋白的X射线晶体结构(3.5Å分辨率)，揭示了其跨膜螺旋排列及钙离子结合位点，阐明了间隙连接通道的开放与关闭调控机制。
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2. **文献名称**：*Expression and purification of functional recombinant human connexin 26 in E. coli*
**作者**：Kreir M. et al. (2008)
**摘要**：开发了一种在大肠杆菌中高效表达重组CX26蛋白的方法，通过脂质体重组形成功能性通道，并验证其电生理特性，为后续药物筛选提供模型。
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3. **文献名称**：*Disease-linked mutations in connexin 26 alter the conformational dynamics of the channel*
**作者**：Thimm J. et al. (2013)
**摘要**：利用重组CX26蛋白结合荧光共振能量转移(FRET)技术，分析致病突变体(如V84L)对通道构象动力学的影响，揭示其导致听力障碍的分子机制。
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如需更多文献细节或具体应用方向的研究，可进一步说明需求。
**Background of CX26 Recombinant Protein**
CX26. or Connexin 26. is a member of the connexin family of transmembrane proteins that form gap junctions, critical intercellular channels enabling direct communication between adjacent cells. These channels facilitate the exchange of ions, metabolites, and signaling molecules (≤1 kDa), supporting tissue homeostasis and coordinated cellular responses. CX26 is encoded by the *GJB2* gene and is highly expressed in epithelial tissues, the inner ear, and skin, where it plays roles in cochlear function, wound healing, and epidermal differentiation.
Mutations in *GJB2* are linked to hereditary hearing loss (e.g., nonsyndromic deafness DFNB1) and skin disorders like keratitis-ichthyosis-deafness (KID) syndrome. To study these pathologies, recombinant CX26 proteins are produced using heterologous expression systems (e.g., *E. coli*, mammalian cells). Recombinant CX26 retains structural features, including four transmembrane domains, two extracellular loops, and cytoplasmic N-/C-termini, enabling in vitro analysis of channel assembly, gating, and interactions.
Researchers use CX26 recombinant proteins to investigate disease-associated mutations, screen therapeutic compounds, and develop biosensors. Its purified form also aids structural studies (e.g., cryo-EM) to resolve channel conformations and mechanisms. Beyond basic research, recombinant CX26 holds potential for designing gene therapies or pharmacological modulators targeting connexin-related disorders.
In summary, CX26 recombinant protein serves as a vital tool for dissecting gap junction biology, elucidating disease mechanisms, and advancing translational applications in hearing and skin diseases.
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