| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CX37 |
| Uniprot No | P35212 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-333aa |
| 氨基酸序列 | MGDWGFLEKLLDQVQEHSTVVGKIWLTVLFIFRILILGLAGESVWGDEQSDFECNTAQPGCTNVCYDQAFPISHIRYWVLQFLFVSTPTLVYLGHVIYLSRREERLRQKEGELRALPAKDPQVERALAAVERQMAKISVAEDGRLRIRGALMGTYVASVLCKSVLEAGFLYGQWRLYGWTMEPVFVCQRAPCPYLVDCFVSRPTEKTIFIIFMLVVGLISLVLNLLELVHLLCRCLSRGMRARQGQDAPPTQGTSSDPYTDQVFFYLPVGQGPSSPPCPTYNGLSSSEQNWANLTTEERLASSRPPLFLDPPPQNGQKPPSRPSSSASKKQYV |
| 预测分子量 | 37,4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CX37重组蛋白的3篇参考文献示例：
1. **文献名称**: "Expression and functional characterization of recombinant connexin37 in mammalian cells"
**作者**: Gabriela S. et al.
**摘要**: 该研究在HEK293细胞中成功表达了重组CX37蛋白，通过膜片钳技术证实其形成功能性间隙连接通道，并发现其电导特性受磷酸化调控，为体外研究CX37生理作用提供了模型。
2. **文献名称**: "Connexin37 regulates platelet aggregation through modulation of ATP release"
**作者**: Wong C.W. et al.
**摘要**: 文章利用重组CX37蛋白发现其在小鼠血小板中调控ATP释放，影响血栓形成过程，基因敲除实验显示CX37缺失导致血小板聚集异常，提示其在心血管疾病中的潜在治疗价值。
3. **文献名称**: "Structural insights into the Connexin37 hemichannel using cryo-EM"
**作者**: Beyer E.C. & Patel D.
**摘要**: 通过冷冻电镜解析了重组CX37蛋白的3D结构(分辨率3.2Å)，揭示了其独特的孔道构象及钙离子结合位点，为设计靶向CX37通道的调节剂奠定结构基础。
(注：以上文献信息为示例性质，实际引用时请核实真实文献)
**Background of CX37 Recombinant Protein**
CX37. also known as Connexin 37 (Cx37), is a member of the connexin family of transmembrane proteins that form gap junctions, which are critical for direct intercellular communication. These channels facilitate the transfer of ions, metabolites, and signaling molecules (≤1 kDa) between adjacent cells, regulating processes such as tissue homeostasis, electrical coupling, and coordinated cellular responses. Structurally, Cx37 consists of four transmembrane domains, two extracellular loops, and intracellular N- and C-termini. Six connexin subunits oligomerize to form a connexon (hemichannel), which pairs with a connexon from a neighboring cell to create a functional gap junction channel.
Cx37 is predominantly expressed in vascular endothelial cells, immune cells (e.g., macrophages), and reproductive tissues. It plays roles in vascular function, inflammation modulation, and ovarian follicle development. Studies highlight its involvement in atherosclerosis, where Cx37 gap junctions influence endothelial permeability and leukocyte adhesion. In reproductive biology, Cx37 is essential for oocyte maturation and communication between granulosa cells and oocytes.
The recombinant CX37 protein is produced using expression systems (e.g., *E. coli* or mammalian cells) to enable *in vitro* studies of its structure-function relationships, channel dynamics, and interactions. Recombinant forms allow researchers to investigate disease-associated mutations, screen for modulators, or develop therapeutic strategies targeting aberrant gap junction activity. For instance, Cx37 polymorphisms have been linked to cardiovascular risks, underscoring its clinical relevance.
Overall, CX37 recombinant protein serves as a vital tool for dissecting the molecular mechanisms of connexin-mediated communication and its implications in health and disease. Its study bridges fundamental biology and translational research, offering insights into potential therapies for vascular, inflammatory, and reproductive disorders.
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