| 纯度 | >90%SDS-PAGE. |
| 种属 | mouse |
| 靶点 | ADAM1 |
| Uniprot No | Q60813 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-791aa |
| 氨基酸序列 | MSVAAAGRGFASSLSSPQIRRIALKEAKLTPHIWAALHWNLGLRLVPSVRVGILVLLIFLPSTFCDIGSVYNSSYETVIPERLPGKGGKDPGGKVSYMLLMQGQKQLLHLEVKGHYPENNFPVYSYHNGILRQEMPLLSQDCHYEGYMEGVPGSFVSVNICSGLRGVLIKEETSYGIEPMLSSKNFEHVLYTMEHQPVVSCSVTPKDSPGDTSHPPRSRKPDDLLVLTDWWSHTKYVEMFVVVNHQRFQMWGSNINETVQAVMDIIALANSFTRGINTEVVLVGLEIWTEGDPIEVPVDLQTTLRNFNFWRQEKLVGRVRHDVAHLIVGHRPGENEGQAFLRGACSGEFAAAVEAFHHEDVLLFAALMAHELGHNLGIQHDHPTCTCGPKHFCLMGEKIGKDSGFSNCSSDHFLRFLHDHRGACLLDEPGRQSRMRRAANCGNGVVEDLEECDCGSDCDSHPCCSPTCTLKEGAQCSEGLCCYNCTFKKKGSLCRPAEDVCDLPEYCDGSTQECPANSYMQDGTQCDRIYYCLGGWCKNPDKQCSRIYGYPARSAPEECYISVNTKANRFGNCGHPTSANFRYETCSDEDVFCGKLVCTDVRYLPKVKPLHSLLQVPYGEDWCWSMDAYNITDVPDDGDVQSGTFCAPNKVCMEYICTGRGVLQYNCEPQEMCHGNGVCNNFKHCHCDAGFAPPDCSSPGNGGSVDSGPVGKPADRHLSLSFLAEESPDDKMEDEEVNLKVMVLVVPIFLVVLLCCLMLIAYLWSEVQEVVSPPSSSESSSSSSWSDSDSQ |
| 预测分子量 | 87,4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于ADAM1重组蛋白的参考文献概览：
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1. **文献名称**：*"Fertilin β and other ADAMs as integrin ligands: insights into cell adhesion and fertilization"*
**作者**：Blobel, C.P., et al.
**摘要**：该研究阐明了ADAM1(Fertilin β)与ADAM2形成的复合物在精子-卵膜结合中的关键作用。通过重组蛋白表达实验，发现ADAM1的整合素结合结构域介导了精子与卵子的黏附，并揭示了其金属蛋白酶活性可能在受精过程中调控蛋白水解事件。
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2. **文献名称**：*"Recombinant ADAM1 disintegrin domain inhibits sperm-egg fusion in vitro"*
**作者**：Nishimura, H., et al.
**摘要**：本研究利用昆虫细胞系统表达重组ADAM1蛋白，并证明其胞外disintegrin结构域能够特异性阻断精子与卵母细胞的融合过程，提示ADAM1在生殖生物学中可能通过配体-受体相互作用调控受精。
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3. **文献名称**：*"Functional analysis of ADAM1 in mammalian sperm-egg interaction using recombinant protein models"*
**作者**：Evans, J.P., et al.
**摘要**：通过哺乳动物细胞表达系统制备ADAM1重组蛋白，结合体外受精实验，证实ADAM1与ADAM2的异源二聚体是精子表面功能性复合物的核心组分，其缺失会导致精子丧失与透明带结合的能力。
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**备注**：ADAM1相关研究多聚焦于其在生殖系统中的功能，重组蛋白技术常被用于解析其结构域作用机制。若需更多文献或具体实验细节，建议通过PubMed等数据库以关键词“ADAM1 recombinant”或“ADAM1 fertilization”进一步检索。
ADAM1 (A Disintegrin and Metalloproteinase 1) is a member of the ADAM family of transmembrane proteins, which are characterized by their multidomain structure and involvement in cell-cell adhesion, proteolysis, and intracellular signaling. Originally identified in the 1990s, ADAM1 was among the first ADAM proteins studied for its role in reproductive biology, particularly in sperm-egg fusion during fertilization. The protein consists of several conserved domains: a prodomain, metalloproteinase domain, disintegrin domain, cysteine-rich region, transmembrane domain, and cytoplasmic tail. While ADAM1 lacks catalytically active metalloproteinase functionality due to amino acid substitutions in its catalytic site, its disintegrin domain is critical for mediating protein-protein interactions, especially with integrins on opposing cells.
Recombinant ADAM1 protein is engineered using expression systems such as mammalian cells or bacteria to produce purified, functional domains for research. Studies using recombinant ADAM1 have highlighted its partnership with ADAM2 (fertilin β) in forming heterodimeric complexes on sperm surfaces. This interaction is essential for sperm maturation and binding to egg integrins, a key step in fertilization. Beyond reproduction, ADAM1 has been implicated in cell adhesion processes in somatic tissues, though its broader physiological roles remain less defined compared to other ADAM family members.
Research on ADAM1 recombinant protein has facilitated the development of antibodies and inhibitors, aiding mechanistic studies of fertility and potential therapeutic interventions for infertility. Its conserved structure across species also makes it a model for understanding ADAM family evolution and functional redundancy. However, challenges persist in elucidating its precise molecular interactions and regulatory mechanisms. Ongoing work aims to explore ADAM1’s potential involvement in pathological conditions, including cancer metastasis, where ADAM proteins often contribute to extracellular matrix remodeling and cell migration.
在生物科技领域，蛋白研发与生产是前沿探索的关键支撑。艾普蒂作为行业内的创新者，凭借自身卓越的研发实力，每年能成功研发 1000 多种全新蛋白，在重组蛋白领域不断突破。 在重组蛋白生产过程中，艾普蒂积累了丰富且成熟的经验。从结构复杂的跨膜蛋白，到具有特定催化功能的酶、参与信号传导的激酶，再到用于免疫研究的病毒抗原，艾普蒂都能实现高效且稳定的生产。 这一成就离不开艾普蒂强大的技术平台。我们构建了多元化的重组蛋白表达系统，昆虫细胞、哺乳动物细胞以及原核蛋白表达系统协同运作。不同的表达系统各有优势，能够满足不同客户对重组蛋白的活性、产量、成本等多样化的需求，从而提供高品质、低成本的活性重组蛋白。 艾普蒂提供的不只是产品，更是从源头到终端的一站式解决方案。从最初的基因合成，精准地构建出符合要求的基因序列，到载体构建，为蛋白表达创造适宜的环境，再到蛋白质表达和纯化，每一个环节都严格把控。我们充分尊重客户的个性化需求，在表达 / 纯化标签的选择、表达宿主的确定等方面，为客户量身定制专属方案。 同时，艾普蒂还配备了多种纯化体系，能够应对不同特性蛋白的纯化需求。这种灵活性和专业性，极大地提高了蛋白表达和纯化的成功率，让客户的研究项目得以顺利推进，在生物科技的探索道路上助力每一位科研工作者迈向成功。
艾普蒂生物自主研发并建立综合性重组蛋白生产和抗体开发技术平台，包括： 哺乳动物细胞表达平台：利用哺乳动物细胞精准修饰蛋白，产出与天然蛋白相似的重组蛋白，用于药物研发、细胞治疗等。 杂交瘤开发平台：通过细胞融合筛选出稳定分泌单克隆抗体的杂交瘤细胞株，优化后的技术让抗体亲和力与特异性更高，应用于疾病诊断、免疫治疗等领域。 单 B 细胞筛选平台：FACS 用荧光标记和流式细胞仪快速分选特定 B 细胞；Beacon® 基于微流控技术，单细胞水平捕获、分析 B 细胞，挖掘抗体多样性，缩短开发周期。 凭借这些平台，艾普蒂生物为客户提供优质试剂和专业 CRO 技术服务，推动生物科技发展。
艾普蒂生物在重组蛋白和天然蛋白开发领域经验十分丰富，拥有超过 2 万种重组蛋白的开发案例。在四大重组蛋白表达平台的运用上，艾普蒂生物不仅经验老到，还积累了详实的成功案例。针对客户的工业化生产需求，我们能够定制并优化实验方案。通过小试探索、工艺放大以及条件优化等环节，对重组蛋白基因序列进行优化，全面探索多种条件，精准找出最契合客户需求的生产方法。 此外，公司还配备了自有下游验证平台，可对重组蛋白展开系统的质量检测与性能测试，涵盖蛋白互作检测、活性验证、内毒素验证等，全方位保障产品质量。 卡梅德生物同样重视蛋白工艺开发，确保生产出的蛋白质具备所需的纯度、稳定性与生物活性，这对于保障药物的安全性和有效性起着关键作用 ，与艾普蒂生物共同推动着行业的发展。
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