| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ADAM20 |
| Uniprot No | O43506 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-726aa |
| 氨基酸序列 | MAVGEPLVHIRVTLLLLWFGMFLSISGHSQARPSQYFTSPEVVIPLKVISRGRGAKAPGWLSYSLRFGGQRYIVHMRVNKLLFAAHLPVFTYTEQHALLQDQPFIQDDCYYHGYVEGVPESLVALSTCSGGFLGMLQINDLVYEIKPISVSATFEHLVYKIDSDDTQFPPMRCGLTEEKIAHQMELQLSYNFTLKQSSFVGWWTHQRFVELVVVVDNIRYLFSQSNATTVQHEVFNVVNIVDSFYHPLEVDVILTGIDIWTASNPLPTSGDLDNVLEDFSIWKNYNLNNRLQHDVAHLFIKDTQGMKLGVAYVKGICQNPFNTGVDVFEDNRLVVFAITLGHELGHNLGMQHDTQWCVCELQWCIMHAYRKVTTKFSNCSYAQYWDSTISSGLCIQPPPYPGNIFRLKYCGNLVVEEGEECDCGTIRQCAKDPCCLLNCTLHPGAACAFGICCKDCKFLPSGTLCRQQVGECDLPEWCNGTSHQCPDDVYVQDGISCNVNAFCYEKTCNNHDIQCKEIFGQDARSASQSCYQEINTQGNRFGHCGIVGTTYVKCWTPDIMCGRVQCENVGVIPNLIEHSTVQQFHLNDTTCWGTDYHLGMAIPDIGEVKDGTVCGPEKICIRKKCASMVHLSQACQPKTCNMRGICNNKQHCHCNHEWAPPYCKDKGYGGSADSGPPPKNNMEGLNVMGKLRYLSLLCLLPLVAFLLFCLHVLFKKRTKSKEDEEG |
| 预测分子量 | 81,6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ADAM20重组蛋白的3篇参考文献示例(注：文献为虚构，仅作格式参考)：
1. **标题**：*Recombinant Expression and Functional Characterization of ADAM20 in Sperm Maturation*
**作者**：Smith JL, et al.
**摘要**：研究成功在大肠杆菌中表达并纯化重组ADAM20蛋白，证实其在体外具有金属蛋白酶活性，并参与精子表面蛋白的加工，提示其在生殖系统中的潜在作用。
2. **标题**：*Structural Insights into ADAM20 via Recombinant Protein Crystallography*
**作者**：Wang Y, et al.
**摘要**：通过昆虫细胞系统表达重组人源ADAM20胞外域，解析其晶体结构，揭示了其底物结合域的独特构象，为设计靶向抑制剂提供结构基础。
3. **标题**：*ADAM20 Recombinant Protein Modulates Cancer Cell Invasion in vitro*
**作者**：Garcia R, et al.
**摘要**：利用哺乳动物细胞表达的重组ADAM20蛋白，发现其通过切割特定细胞外基质蛋白(如纤连蛋白)促进肿瘤细胞迁移，提示其与癌症转移的相关性。
(以上内容为模拟示例，实际文献需通过学术数据库检索确认。)
**Background of ADAM20 Recombinant Protein**
ADAM20 (A Disintegrin and Metalloproteinase 20) is a member of the ADAM family of transmembrane proteins, which are characterized by their dual roles in proteolysis and cell adhesion. These proteins typically consist of multiple functional domains, including a prodomain, metalloproteinase, disintegrin-like, cysteine-rich, transmembrane, and cytoplasmic tail regions. ADAM20. specifically, is implicated in ectodomain shedding—a process where membrane-bound proteins are cleaved to release soluble bioactive molecules—and cell-cell interactions. While its biological functions remain less characterized compared to other ADAMs (e.g., ADAM17 or ADAM10), studies suggest its potential involvement in reproductive biology, immune regulation, and cancer progression.
The recombinant ADAM20 protein is engineered using expression systems such as mammalian or insect cells to ensure proper post-translational modifications and functional activity. This recombinant form allows researchers to study ADAM20's enzymatic properties, substrate specificity, and regulatory mechanisms in vitro. For instance, it has been utilized to investigate its role in processing substrates like growth factors, cytokines, or adhesion molecules, which may influence signaling pathways in development or disease.
ADAM20 is of particular interest in reproductive health due to its expression in testicular tissues and possible involvement in sperm maturation. Additionally, its dysregulation has been observed in certain cancers, linking it to tumor invasion and metastasis. However, its exact physiological and pathological roles require further exploration. Recombinant ADAM20 serves as a critical tool for unraveling these mechanisms, offering insights into targeted therapeutic strategies for conditions associated with ADAM20 dysfunction. Research continues to focus on elucidating its interactions, inhibitors, and tissue-specific functions to expand its biomedical relevance.
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