| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ADAM22 |
| Uniprot No | Q9P0K1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-906aa |
| 氨基酸序列 | MQAAVAVSVPFLLLCVLGTCPPARCGQAGDASLMELEKRKENRFVERQSIVPLRLIYRSGGEDESRHDALDTRVRGDLGGPQLTHVDQASFQVDAFGTSFILDVVLNHDLLSSEYIERHIEHGGKTVEVKGGEHCYYQGHIRGNPDSFVALSTCHGLHGMFYDGNHTYLIEPEENDTTQEDFHFHSVYKSRLFEFSLDDLPSEFQQVNITPSKFILKPRPKRSKRQLRRYPRNVEEETKYIELMIVNDHLMFKKHRLSVVHTNTYAKSVVNMADLIYKDQLKTRIVLVAMETWATDNKFAISENPLITLREFMKYRRDFIKEKSDAVHLFSGSQFESSRSGAAYIGGICSLLKGGGVNEFGKTDLMAVTLAQSLAHNIGIISDKRKLASGECKCEDTWSGCIMGDTGYYLPKKFTQCNIEEYHDFLNSGGGACLFNKPSKLLDPPECGNGFIETGEECDCGTPAECVLEGAECCKKCTLTQDSQCSDGLCCKKCKFQPMGTVCREAVNDCDIRETCSGNSSQCAPNIHKMDGYSCDGVQGICFGGRCKTRDRQCKYIWGQKVTASDKYCYEKLNIEGTEKGNCGKDKDTWIQCNKRDVLCGYLLCTNIGNIPRLGELDGEITSTLVVQQGRTLNCSGGHVKLEEDVDLGYVEDGTPCGPQMMCLEHRCLPVASFNFSTCLSSKEGTICSGNGVCSNELKCVCNRHWIGSDCNTYFPHNDDAKTGITLSGNGVAGTNIIIGIIAGTILVLALILGITAWGYKNYREQRQLPQGDYVKKPGDGDSFYSDIPPGVSTNSASSSKKRSNGLSHSWSERIPDTKHISDICENGRPRSNSWQGNLGGNKKKIRGKRFRPRSNSTETLSPAKSPSSSTGSIASSRKYPYPMPPLPDEDKKVNRQSARLWETSI |
| 预测分子量 | 100,4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ADAM22重组蛋白的三篇示例参考文献(内容基于真实研究领域，但具体标题和作者为模拟概括)：
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1. **文献名称**: *ADAM22 interacts with LGI1 to regulate synaptic transmission and epilepsy susceptibility*
**作者**: Fukata Y., et al.
**摘要**: 该研究揭示了ADAM22作为突触后蛋白，通过与抗癫痫相关蛋白LGI1的结合，调控突触的兴奋性传递。重组ADAM22的体外实验证实其与LGI1直接相互作用，并参与维持突触AMPAR受体稳定性，突变体可导致小鼠癫痫表型。
2. **文献名称**: *Structural basis of ADAM22 extracellular domain organization and its role in Kv1 channel clustering*
**作者**: Suzuki K., Higuchi T., et al.
**摘要**: 本研究通过重组表达ADAM22胞外域蛋白，利用X射线晶体学解析其三维结构，发现其金属蛋白酶样结构域介导与电压门控钾通道Kv1的相互作用，为ADAM22在神经元轴突初始段调控离子通道分布提供了结构依据。
3. **文献名称**: *Recombinant ADAM22 ectodomain binds neurotoxin BmK IT2 to modulate neuronal excitability*
**作者**: Liu Y., Shao Z., et al.
**摘要**: 通过重组表达ADAM22胞外区蛋白，发现其与蝎毒素BmK IT2特异性结合，阻断毒素对钠通道的抑制作用，表明ADAM22可能在神经兴奋性调控中具有解毒或信号调节功能。
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**备注**：以上文献信息为示例，实际引用需通过PubMed、Google Scholar等平台以“ADAM22 recombinant protein”等关键词检索，并核实具体作者及摘要内容。研究领域多集中于ADAM22的突触功能、离子通道互作及神经系统疾病机制。
**Background of ADAM22 Recombinant Protein**
ADAM22 (A Disintegrin and Metalloproteinase 22) is a member of the ADAM family of transmembrane proteins, which are characterized by their multi-domain structure involving metalloprotease, disintegrin, cysteine-rich, and epidermal growth factor (EGF)-like domains. While many ADAMs function as proteases or cell adhesion molecules, ADAM22 lacks enzymatic activity due to mutations in its catalytic site. Instead, it acts as a receptor or scaffold protein, primarily in the nervous system.
ADAM22 is critical for synaptic organization and neurotransmission. It interacts with extracellular ligands like leucine-rich glioma-inactivated 1 (LGI1) and postsynaptic proteins such as PSD-95. linking cell-surface interactions to intracellular signaling. This interaction stabilizes voltage-gated potassium channels (Kv1) at juxtaparanodes, ensuring proper neuronal excitability. Mutations in ADAM22 or its binding partners are linked to neurological disorders, including epilepsy, autoimmune encephalitis, and schizophrenia.
Recombinant ADAM22 protein is engineered for *in vitro* studies to dissect its structural and functional roles. Produced via mammalian or insect cell systems, it retains key domains (e.g., disintegrin, cysteine-rich regions) for ligand binding and complex formation. Researchers use it to study protein-protein interactions, synaptic mechanisms, and pathogenic autoantibodies in encephalitis. Its soluble form, often fused with tags like Fc or His, facilitates purification and detection in assays.
In disease models, recombinant ADAM22 helps explore therapeutic strategies, such as blocking pathogenic antibodies or restoring synaptic function. Its role in cancer is also emerging, as ADAM22 may influence tumor progression via cell adhesion signaling. Overall, recombinant ADAM22 serves as a vital tool for unraveling neurological pathways and developing targeted therapies.
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