| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | IL8Ra |
| Uniprot No | P25025 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-40aa |
| 氨基酸序列 | MEDFNMESDSFEDFWKGEDLSNYSYSSTLPPFLLDAAPCE |
| 预测分子量 | 20.6kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于IL8Ra(CXCR1)重组蛋白的3篇文献示例(内容基于学术研究主题模拟，非真实文献)：
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1. **文献名称**：*Expression and Functional Characterization of Recombinant Human IL8Ra in Mammalian Cells*
**作者**：Smith J, et al.
**摘要**：该研究成功在HEK293细胞中表达了重组人IL8Ra蛋白，并通过流式细胞术和钙离子流实验验证了其与IL-8配体的结合活性及信号转导功能，为后续受体拮抗剂筛选提供了实验基础。
2. **文献名称**：*Structural Insights into IL8Ra Activation Mechanism via Cryo-EM Analysis*
**作者**：Zhang L, et al.
**摘要**：利用冷冻电镜解析了IL8Ra与其天然配体IL-8结合的复合物结构，揭示了受体激活的关键构象变化，为靶向IL8Ra的炎症性疾病药物设计提供了结构依据。
3. **文献名称**：*Purification Optimization of Recombinant IL8Ra from E. coli for Antibody Production*
**作者**：Kim S, et al.
**摘要**：通过优化大肠杆菌表达系统的诱导条件和层析纯化策略，高效获取高纯度IL8Ra胞外域蛋白，并用于制备特异性单克隆抗体，验证了其在免疫检测中的应用潜力。
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(注：上述文献为示例性质，实际引用需查询PubMed、Web of Science等数据库获取真实信息。)
Interleukin-8 receptor alpha (IL8Ra), also known as CXCR1. is a chemokine receptor belonging to the G protein-coupled receptor (GPCR) superfamily. It is primarily expressed on the surface of neutrophils, endothelial cells, and certain immune cells. IL8Ra specifically binds to interleukin-8 (IL-8/CXCL8), a pro-inflammatory chemokine involved in neutrophil activation, chemotaxis, and degranulation during inflammatory responses. Structurally, it features seven transmembrane domains, an extracellular N-terminus critical for ligand binding, and intracellular regions that mediate downstream signaling via G proteins.
Recombinant IL8Ra protein is engineered in vitro using expression systems such as mammalian cells or bacteria, ensuring proper folding and post-translational modifications. This protein retains the functional domains required for ligand interaction and signaling studies. Researchers utilize recombinant IL8Ra to investigate receptor-ligand binding kinetics, signal transduction pathways (e.g., MAPK/ERK, PI3K/Akt), and its role in diseases like chronic inflammation, autoimmune disorders, and cancer metastasis. In cancer biology, IL8Ra overexpression is linked to angiogenesis and tumor cell migration, making it a potential therapeutic target. Additionally, it serves as a tool for developing antagonists or antibodies to modulate IL-8-mediated pathologies. Its study provides insights into immune regulation and therapeutic strategies for inflammation-driven diseases.
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