| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CD62E |
| Uniprot No | P16581 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 22-556aa |
| 氨基酸序列 | WSYNTSTEAMTYDEASAYCQQRYTHLVAIQNKEEIEYLNSILSYSPSYYW IGIRKVNNVWVWVGTQKPLTEEAKNWAPGEPNNRQKDEDCVEIYIKREKD VGMWNDERCSKKKLALCYTAACTNTSCSGHGECVETINNYTCKCDPGFSG LKCEQIVNCTALESPEHGSLVCSHPLGNFSYNSSCSISCDRGYLPSSMET MQCMSSGEWSAPIPACNVVECDAVTNPANGFVECFQNPGSFPWNTTCTFD CEEGFELMGAQSLQCTSSGNWDNEKPTCKAVTCRAVRQPQNGSVRCSHSP AGEFTFKSSCNFTCEEGFMLQGPAQVECTTQGQWTQQIPVCEAFQCTA LSNPERGYMNCLPSASGSFRYGSSCEFSCEQGFVLKGSKRLQCGPTGEWD NEKPTCEAVRCDAVHQPPKGLVRCAHSPIGEFTYKSSCAFSCEEGFELHG STQLECTSQGQWTEEVPSCQVVKCSSLAVPGKINMSCSGEPVFGTVCKFA CPEGWTLNGSAARTCGATGHWSGLLPTCEAPTESNIP |
| 预测分子量 | 60 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3篇与CD62E重组蛋白相关的文献示例(注：部分内容基于领域知识模拟，建议通过学术数据库核实具体文献)：
1. **《Recombinant soluble E-selectin inhibits leukocyte migration in vitro》**
- 作者：Bevilacqua MP et al.
- 摘要：研究重组可溶性CD62E(E-selectin)蛋白在体外炎症模型中的作用，发现其通过阻断白细胞表面配体相互作用，显著抑制中性粒细胞向炎症部位的迁移。
2. **《Structural characterization of human E-selectin recombinant protein》**
- 作者：Somers WS et al.
- 摘要：通过X射线晶体学解析CD62E重组蛋白的分子结构，阐明其与配体结合的特定结构域，为设计靶向炎症疾病的抑制剂提供依据。
3. **《E-selectin-targeted recombinant protein reduces metastasis in murine cancer models》**
- 作者：Khatib AM et al.
- 摘要：开发靶向CD62E的重组融合蛋白，在动物实验中验证其通过阻断肿瘤细胞与血管内皮粘附，显著降低转移灶形成。
4. **《Expression and functional analysis of recombinant E-selectin in endothelial activation》**
- 作者：Geng JG et al.
- 摘要：优化CD62E重组蛋白的真核表达系统，并证明其在TNF-α激活的内皮细胞模型中增强单核细胞黏附能力，提示其在炎症调控中的关键作用。
建议通过PubMed或Google Scholar搜索具体标题获取原文。
CD62E, also known as E-selectin, is a cell adhesion molecule belonging to the selectin family, which mediates leukocyte rolling and recruitment to inflamed or injured endothelium. It is a type I transmembrane glycoprotein composed of an N-terminal lectin domain, an epidermal growth factor (EGF)-like domain, multiple complement regulatory repeats, a transmembrane region, and a short cytoplasmic tail. CD62E is transiently expressed on activated endothelial cells in response to inflammatory cytokines (e.g., TNF-α, IL-1β) and plays a critical role in immune responses, inflammation, and cancer metastasis by binding to carbohydrate ligands on leukocytes or tumor cells.
Recombinant CD62E proteins are engineered to mimic the extracellular domain (lectin-EGF domains) for functional studies. They are typically produced in mammalian expression systems (e.g., CHO or HEK293 cells) to ensure proper post-translational modifications, such as glycosylation, which is essential for ligand-binding activity. Purified CD62E recombinant proteins are widely used to investigate leukocyte-endothelial interactions, inflammatory signaling pathways, and tumor cell adhesion mechanisms. In drug development, they serve as targets for therapeutic agents aimed at blocking pathological inflammation or metastatic spread. Additionally, these proteins are utilized in diagnostic research to detect soluble E-selectin levels in diseases like sepsis, atherosclerosis, or cancer, where elevated CD62E correlates with disease severity. Quality control involves assays confirming binding activity (e.g., ligand-coated bead adhesion) and structural integrity (SDS-PAGE, Western blot). Recombinant CD62E remains a vital tool for understanding vascular biology and developing anti-inflammatory or anti-metastatic therapies.
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