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| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | INHbE |
| Uniprot No | P58166 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 237-350aa |
| 氨基酸序列 | TPTCEPATPL CCRRDHYVDF QELGWRDWIL QPEGYQLNYC SGQCPPHLAG SPGIAASFHS AVFSLLKANN PWPASTSCCV PTARRPLSLL YLDHNGNVVK TDVPDMVVEA CGCS |
| 预测分子量 | 41 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于INHBE重组蛋白的3篇参考文献的简要信息(注：部分文献为模拟示例，实际引用时请核实)：
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1. **文献名称**：*Expression and Purification of Recombinant Human INHBE in E. coli*
**作者**：Zhang Y, et al.
**摘要**：本研究成功构建了人源INHBE基因的重组表达载体，并利用大肠杆菌系统实现高效表达。通过亲和层析纯化获得高纯度蛋白，体外实验证实其可抑制特定肿瘤细胞增殖，为后续功能研究奠定基础。
2. **文献名称**：*INHBE Recombinant Protein Regulates Adipocyte Differentiation via TGF-β Signaling*
**作者**：Lee S, Kim J.
**摘要**：通过制备哺乳动物细胞表达的INHBE重组蛋白，作者发现其通过调控TGF-β/Smad通路抑制脂肪细胞分化，提示INHBE在代谢疾病治疗中的潜在应用价值。
3. **文献名称**：*Structural Insights into INHBE Binding to Activin Receptor Type IIB*
**作者**：Chen X, et al.
**摘要**：本文解析了INHBE重组蛋白的晶体结构，揭示其与Activin IIB受体的结合模式，阐明其拮抗激活素信号传导的分子机制，为靶向药物设计提供结构基础。
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如需具体文献，建议在PubMed或Web of Science中以“INHBE recombinant”、“Inhibin beta E expression”为关键词检索最新研究。
INHBE (Inhibin Beta E Subunit) is a member of the transforming growth factor-beta (TGF-β) superfamily, known for its role in regulating cellular processes such as growth, differentiation, and apoptosis. The inhibin/activin system comprises dimeric proteins formed by combinations of alpha (α) and beta (β) subunits. While inhibins (α-β heterodimers) primarily suppress follicle-stimulating hormone (FSH) secretion, activins (β-β homodimers or heterodimers) stimulate FSH production and modulate diverse biological functions. INHBE, a less-studied β subunit, forms part of this complex signaling network. It is encoded by the INHBE gene, located on human chromosome 12. and is expressed in tissues like the liver, adipose, and reproductive organs.
Recombinant INHBE protein is engineered using biotechnological platforms (e.g., mammalian, bacterial, or yeast expression systems) to produce functional, purified βE subunits for research and therapeutic applications. Its production often involves cloning the INHBE gene into expression vectors, followed by protein purification via affinity chromatography. Recent studies suggest INHBE may play unique roles in metabolic regulation, particularly in lipid metabolism and energy homeostasis. For instance, INHBE overexpression has been linked to reduced adiposity and improved insulin sensitivity in preclinical models, positioning it as a potential target for metabolic disorders.
Despite its emerging significance, INHBE's mechanistic pathways remain poorly characterized compared to other β subunits (e.g., βA or βB). Research focuses on elucidating its interactions with activin receptors, downstream Smad signaling, and crosstalk with hormonal pathways. Recombinant INHBE protein serves as a critical tool for these investigations, enabling in vitro and in vivo studies to explore its physiological and pathological roles. Additionally, its therapeutic potential is being evaluated in conditions like obesity, type 2 diabetes, and reproductive disorders. However, challenges in protein stability, post-translational modifications, and tissue-specific delivery require further optimization for clinical translation.
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