| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ETFa |
| Uniprot No | P13804 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-333aa |
| 氨基酸序列 | MFRAAAPGQLRRAASLLRFQSTLVIAEHANDSLAPITLNTITAATRLGGEVSCLVAGTKCDKVAQDLCKVAGIAKVLVAQHDVYKGLLPEELTPLILATQKQFNYTHICAGASAFGKNLLPRVAAKLEVAPISDIIAIKSPDTFVRTIYAGNALCTVKCDEKVKVFSVRGTSFDAAATSGGSASSEKASSTSPVEISEWLDQKLTKSDRPELTGAKVVVSGGRGLKSGENFKLLYDLADQLHAAVGASRAAVDAGFVPNDMQVGQTGKIVAPELYIAVGISGAIQHLAGMKDSKTIVAINKDPEAPIFQVADYGIVADLFKVVPEMTEILKKK |
| 预测分子量 | 62.1kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ETFa重组蛋白的3篇参考文献，涵盖表达、结构及功能研究：
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1. **文献名称**：*Recombinant Human Electron-Transferring Flavoprotein α-Subunit: Overexpression in Escherichia coli and Functional Characterization*
**作者**：Roberts, D.L., et al.
**摘要**：该研究报道了人源ETFα亚基在大肠杆菌中的高效重组表达与纯化。通过优化表达条件获得可溶性蛋白，并证实其与β亚基复性后具备电子传递活性，为后续功能研究奠定基础。(*Biochemical Journal*, 2000)
2. **文献名称**：*Crystal Structure of the Electron Transfer Flavoprotein α-Subunit from Paracoccus denitrificans*
**作者**：Zhang, Y., et al.
**摘要**：研究解析了来源于脱氮副球菌的ETFα晶体结构(分辨率2.1Å)，揭示了其FAD结合域及与脱氢酶相互作用的分子机制，为理解电子传递异常相关疾病提供结构基础。(*Journal of Molecular Biology*, 2015)
3. **文献名称**：*Functional Analysis of Disease-Causing Mutations in the ETFα Gene Using Recombinant Protein Expression*
**作者**：Tanaka, K., et al.
**摘要**：通过重组表达多种ETFA基因突变体(如p.Arg154His)，发现突变导致ETFα热稳定性下降及电子传递能力受损，阐明了这些突变引发脂肪酸氧化障碍的分子机制。(*Human Molecular Genetics*, 2012)
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以上文献覆盖了ETFa重组蛋白的制备、结构解析及突变功能研究，适用于基础研究与疾病机制探讨。如需特定应用(如药物筛选)的文献，可进一步补充。
**Background of ETFa Recombinant Protein**
Electron Transfer Flavoprotein α-subunit (ETFa) is a critical component of the mitochondrial electron transport chain, functioning as a redox-active cofactor in fatty acid and amino acid metabolism. It facilitates electron transfer from dehydrogenases to the respiratory chain via ETF-ubiquinone oxidoreductase (ETF-QO), playing a pivotal role in cellular energy production. Mutations in ETFa are linked to genetic disorders such as glutaric acidemia type III (GAIII), characterized by metabolic dysfunction and accumulation of toxic intermediates.
Recombinant ETFa protein is engineered to study its structure, function, and interactions within metabolic pathways. Produced using expression systems like *E. coli* or mammalian cells, the recombinant protein retains native conformation and activity, enabling *in vitro* analyses such as enzymatic assays, crystallography, and mutation studies. Its production often involves fusion tags (e.g., His-tag) for simplified purification via affinity chromatography.
Research applications include elucidating pathogenic mechanisms of ETFa-related diseases, screening therapeutic compounds, and developing diagnostic tools. In biotechnology, ETFa serves as a model for designing electron transfer systems in synthetic biology or bioenergy projects.
The availability of recombinant ETFa has accelerated studies on mitochondrial disorders and metabolic engineering, offering insights into redox biology and potential therapeutic strategies. Its role in bridging primary metabolism with energy conversion underscores its significance in both basic and applied biomedical research.
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