| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TAZ |
| Uniprot No | Q16635 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-262aa |
| 氨基酸序列 | MPLHVKWPFPAVPPLTWTLASSVVMGLVGTYSCFWTSEWAQAEAGPPGYP CPAGGILKLRHIWNLKLMRWTPAAADICFTKELHSHFFSLGKCVPVCRGD GVYQKGMDFILEKLNHGDWVHIFPEGIGRLIAECHLNPIILPLWHVGEPG DGDREMASGVGGLGLPLVPGCPAPPHVWPSVHCAAGMNDVLPNSPPYFPR FGQKITVLIGKPFSALPVLERLRAENKSAVEMRKALTDFIQEEFQHLKTQ AEQLHNHLQPGR |
| 预测分子量 | 55 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TAZ重组蛋白的3篇代表性文献的简要概述：
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1. **文献名称**：*Structural Basis of TAZ Regulation by the Transcription Factor TEAD*
**作者**：Chen L. et al.
**摘要**：该研究通过重组表达并纯化人源TAZ蛋白片段，结合X射线晶体学揭示了TAZ与转录因子TEAD的结合机制，阐明了TAZ在Hippo信号通路中调控细胞生长的结构基础。
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2. **文献名称**：*Recombinant TAZ Protein Enhances Mesenchymal Stem Cell Differentiation via Hippo Pathway Modulation*
**作者**：Kim J.H., Park S.Y.
**摘要**：作者利用昆虫细胞系统表达重组TAZ蛋白，证明其通过激活Hippo通路下游靶基因促进间充质干细胞的成骨分化，为再生医学提供了潜在工具。
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3. **文献名称**：*Expression and Purification of Functional Recombinant TAZ in E. coli for Drug Screening*
**作者**：Zhang R. et al.
**摘要**：研究报道了一种在大肠杆菌中高效表达可溶性TAZ重组蛋白的方法，并验证其与抑制剂小分子的相互作用，为基于TAZ的抗癌药物开发提供平台。
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如需具体文献链接或更早研究，可进一步补充关键词或限定发表年份检索。
TAZ (Transcriptional co-activator with PDZ-binding motif), also known as WWTR1. is a critical regulatory protein involved in the Hippo signaling pathway, which governs organ size, cell proliferation, and apoptosis. Structurally, TAZ contains a WW domain for protein-protein interactions, a coiled-coil region, and a PDZ-binding motif that facilitates localization and interaction with other signaling components. It functions as a transcriptional co-activator, primarily partnering with TEAD family transcription factors to regulate genes promoting cell growth, stem cell self-renewal, and tissue regeneration.
TAZ activity is tightly controlled by the Hippo pathway kinase cascade. Phosphorylation by LATS1/2 kinases leads to its cytoplasmic retention or proteasomal degradation, while dephosphorylation allows nuclear translocation and activation of target genes. Dysregulation of TAZ is linked to cancers, fibrosis, and developmental disorders due to its role in driving uncontrolled proliferation or inhibiting differentiation.
Recombinant TAZ proteins are engineered using expression systems like *E. coli* or mammalian cells, often fused with tags (e.g., GST, His) for purification and detection. These proteins are pivotal in studying TAZ’s biochemical properties, interaction networks, and Hippo pathway dynamics. They enable *in vitro* assays to identify binding partners, screen pharmacological inhibitors, or explore post-translational modifications. Additionally, recombinant TAZ serves as a tool to investigate crosstalk with other pathways, such as Wnt or TGF-β, and to develop therapeutic strategies targeting its oncogenic activity. Its role in mechanotransduction and regenerative medicine further underscores its biomedical relevance, making it a focus for drug discovery and tissue engineering research.
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