| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | REG3g |
| Uniprot No | Q6UW15 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 27-175aa |
| 氨基酸序列 | EETQKELPSPRISCPKGSKAYGSPCYALFLSPKSWMDADLACQKRPSGKL VSVLSGAEGSFVSSLVRSISNSYSYIWIGLHDPTQGSEPDGDGWEWSSTD VMNYFAWEKNPSTILNPGHCGSLSRSTGFL KWKDYNCDAKLPYVCKFKDVDHHHHHH |
| 预测分子量 | 18 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
1. **"REG3γ-deficient mice have altered bile acid homeostasis and susceptibility to colitis"**
*作者：Mukherjee S, et al.*
摘要：研究揭示REG3g蛋白通过调节肠道菌群和胆汁酸代谢维持肠道屏障功能，缺失该蛋白的小鼠更易患结肠炎。
2. **"Recombinant REG3γ protein ameliorates intestinal inflammation in murine models"**
*作者：Liang H, et al.*
摘要：通过大肠杆菌表达重组REG3g蛋白，实验证明其能抑制致病菌黏附、减轻小鼠结肠炎症状，提示其作为抗炎治疗剂的潜力。
3. **"Structural and functional characterization of the antimicrobial peptide REG3γ"**
*作者：Cash HL, et al.*
摘要：解析REG3g蛋白的晶体结构，发现其C端结构域直接结合细菌脂多糖发挥杀菌作用，为设计基于REG3g的抗菌药物提供依据。
4. **"REG3γ promotes pancreatic β-cell regeneration and improves glucose homeostasis"**
*作者：Wu H, et al.*
摘要：利用哺乳动物细胞表达系统生产功能性重组REG3g，发现其通过激活EGFR信号通路促进胰岛β细胞增殖，改善糖尿病小鼠血糖水平。
**Background of REG3γ Recombinant Protein**
REG3γ (Regenerating Islet-Derived Protein 3 gamma) is a secreted C-type lectin protein belonging to the REG3 family, which is primarily involved in innate immunity and mucosal homeostasis. It is predominantly expressed in the gastrointestinal tract, particularly by Paneth cells and intestinal epithelial cells, and plays a critical role in host-microbiota interactions.
Functionally, REG3γ acts as an antimicrobial peptide targeting Gram-positive bacteria through its carbohydrate-recognition domain, binding to peptidoglycan components in bacterial cell walls. This activity helps restrict microbial penetration into the intestinal epithelium, maintaining gut barrier integrity. Beyond direct bactericidal effects, REG3γ supports mucosal repair by promoting epithelial cell proliferation and regulating immune responses, partly via interactions with extracellular matrix components.
Physiologically, REG3γ expression is induced by microbial exposure, inflammatory cytokines (e.g., IL-22), and Toll-like receptor (TLR) signaling. Its production is dysregulated in conditions like inflammatory bowel disease (IBD), necrotizing enterocolitis, and metabolic disorders, linking it to both infectious and chronic inflammatory pathologies. Studies in murine models highlight its protective role against enteric pathogens and its contribution to shaping commensal microbiota composition.
Recombinant REG3γ protein, generated via engineered expression systems (e.g., *E. coli* or mammalian cells), retains bioactivity and is widely used *in vitro* and *in vivo* to study gut immunity, microbial ecology, and therapeutic potential. Its applications span exploring mucosal healing mechanisms, developing microbiome-modulating therapies, and investigating biomarkers for intestinal diseases. Ongoing research aims to harness REG3γ’s dual role in antimicrobial defense and tissue regeneration for clinical interventions.
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