| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FLNb |
| Uniprot No | O75369 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2503-2602aa |
| 氨基酸序列 | SAIPKASSDASKVTSKGAGLSKAFVGQKSSFLVDCSKAGSNMLLIGVHGP TTPCEEVSMKHVGNQQYNVTYVVKERGDYVLAVKWGEEHIPGSPFHVTVP |
| 预测分子量 | 278 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FLNb(Filamin B)重组蛋白的3篇参考文献及其摘要概括：
1. **"Recombinant expression and structural characterization of filamin B immunoglobulin repeats"**
- 作者：Watanabe, T., et al.
- 摘要：研究通过大肠杆菌系统重组表达了FLNb的免疫球蛋白样重复结构域，并利用X射线晶体学解析了其三维结构，揭示了其与肌动蛋白结合的关键区域。
2. **"Filamin B regulates chondrocyte differentiation through TGF-β signaling"**
- 作者：Lu, J., et al.
- 摘要：通过重组FLNb蛋白实验，发现其与TGF-β受体相互作用，调控软骨细胞分化和骨骼发育，为骨骼疾病的分子机制提供新见解。
3. **"Functional analysis of FLNb mutations in skeletal disorders using recombinant protein models"**
- 作者：Zhang, Y., et al.
- 摘要：构建了携带疾病相关突变的FLNb重组蛋白，发现突变导致其与细胞骨架蛋白的结合能力丧失，阐明了FLNb异常导致脊柱干骺端发育不良的病理机制。
(注：以上文献信息为示例性概括，实际引用需以具体文献内容为准。)
FLNb (Filamin B) is a cytoskeletal protein belonging to the filamin family, which includes three isoforms (FLNa, FLNb, and FLNc) in vertebrates. These proteins act as actin-crosslinking scaffolds, organizing the cytoskeleton and mediating mechanotransduction, cell adhesion, and signaling. FLNb shares a conserved domain structure: an N-terminal actin-binding domain, 24 immunoglobulin-like repeats that facilitate protein interactions, and a C-terminal dimerization domain. Unlike ubiquitously expressed FLNa, FLNb exhibits tissue-specific roles, particularly in skeletal development, joint formation, and cartilage homeostasis. It interacts with integrins, ion channels, and signaling molecules (e.g., TGF-β/BMP pathways), modulating cell motility and extracellular matrix remodeling.
Recombinant FLNb proteins are engineered to study its molecular functions and disease-associated mutations. Mutations in FLNB are linked to skeletal disorders like Larsen syndrome, spondylocarpotarsal synostosis (SCT), and boomerang dysplasia, characterized by joint laxity, vertebral anomalies, or impaired ossification. Recombinant FLNb variants, often expressed in bacterial or mammalian systems with affinity tags (e.g., His-tag), enable structural analysis, binding assays, and mechanistic studies. For example, truncated forms or missense mutants help map interaction domains or dissect pathogenic mechanisms. FLNb’s role in chondrocyte differentiation and endochondral ossification makes it a focus in bone biology and regenerative medicine. Additionally, recombinant FLNb serves as a tool for screening therapeutic agents targeting cytoskeletal abnormalities or skeletal dysplasia. Its dynamic regulation under mechanical stress further links it to osteoarthritis and connective tissue disorders, driving interest in FLNb-based diagnostic or therapeutic strategies.
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