| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TUBb1 |
| Uniprot No | P68363 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-451aa |
| 氨基酸序列 | MRECISIHVG QAGVQIGNAC WELYCLEHGI QPDGQMPSDK TIGGGDDSFN TFFSETGAGK HVPRAVFVDL EPTVIDEVRT GTYRQLFHPE QLITGKEDAA NNYARGHYTI GKEIIDLVLD RIRKLADQCT GLQGFLVFHS FGGGTGSGFT SLLMERLSVD YGKKSKLEFS IYPAPQVSTA VVEPYNSILT THTTLEHSDC AFMVDNEAIY DICRRNLDIE RPTYTNLNRL ISQIVSSITA SLRFDGALNV DLTEFQTNLV PYPRIHFPLA TYAPVISAEK AYHEQLSVAE ITNACFEPAN QMVKCDPRHG KYMACCLLYR GDVVPKDVNA AIATIKTKRS IQFVDWCPTG FKVGINYQPP TVVPGGDLAK VQRAVCMLSN TTAIAEAWAR LDHKFDLMYA KRAFVHWYVG EGMEEGEFSE AREDMAALEK DYEEVGVDSV EGEGEEEGEE Y |
| 预测分子量 | 50,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与TUBB1重组蛋白相关的文献摘要概括，供参考：
1. **文献名称**：*"Expression and functional characterization of recombinant human β1-tubulin in platelet disorders"*
**作者**：Freson K et al.
**摘要**：本研究通过在大肠杆菌系统中表达重组人源TUBB1蛋白，分析了其与微管聚合的相互作用，揭示了TUBB1基因突变导致血小板形态异常的分子机制，为先天性血小板疾病提供了实验依据。
2. **文献名称**：*"Purification and structural analysis of TUBB1 isoforms in megakaryocytic cells"*
**作者**：Schwer HD et al.
**摘要**：作者利用杆状病毒-昆虫细胞系统重组表达TUBB1蛋白，通过体外微管组装实验证明TUBB1特异性参与巨核细胞中微管骨架的动态调控，并解析了其C端结构域对功能的关键影响。
3. **文献名称**：*"In vitro reconstitution of TUBB1-dependent microtubule dynamics in platelets"*
**作者**：Bender M et al.
**摘要**：该研究通过共表达TUBB1与α-tubulin重组蛋白，在体外重构了血小板微管网络，发现TUBB1的β5亚型通过调控微管稳定性影响血小板活化过程中的形态变化。
*注：以上文献信息为基于领域内典型研究的模拟概括，实际引用时建议通过PubMed或Web of Science核对原文。若需具体文献，可补充提供DOI或PMID进一步检索。*
**Background of TUBB1 Recombinant Protein**
TUBB1 (Tubulin Beta 1 Class VI) is a member of the β-tubulin family, a critical component of microtubules—dynamic cytoskeletal structures essential for eukaryotic cell division, intracellular transport, and maintenance of cell shape. Unlike ubiquitously expressed β-tubulin isoforms, TUBB1 is predominantly found in megakaryocytes and platelets, where it plays a specialized role in platelet formation and function. Mutations in the *TUBB1* gene have been linked to inherited macrothrombocytopenia, a bleeding disorder characterized by enlarged, dysfunctional platelets, underscoring its physiological importance.
Recombinant TUBB1 protein is engineered using heterologous expression systems (e.g., *E. coli*, yeast, or mammalian cells*) to produce high-purity, functional β-tubulin for research. The recombinant form retains key structural features, including the GTP-binding domain, polymerization capacity, and interaction sites for microtubule-associated proteins (MAPs) or antimitotic drugs. Its production often involves affinity tag-based purification (e.g., His-tag) followed by refolding or post-translational modifications to ensure native-like activity.
Studies leveraging recombinant TUBB1 have advanced understanding of microtubule dynamics in platelet biology, particularly in proplatelet formation and cytoskeletal remodeling. It also serves as a tool for investigating tubulin isotype-specific functions, such as how TUBB1 mutations disrupt microtubule stability, leading to thrombocytopenia. Additionally, this protein is utilized in drug discovery, screening compounds targeting β-tubulin in cancers or platelet disorders, and in structural studies (e.g., cryo-EM) to map drug-binding sites.
Overall, recombinant TUBB1 bridges molecular insights into tubulin biology with translational applications in hematology and oncology.
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