| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HNRPK |
| Uniprot No | P61978 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-463aa |
| 氨基酸序列 | METEQPEETFPNTETNGEFGKRPAEDMEEEQAFKRSRNTDEMVELRILLQ SKNAGAVIGKGGKNIKALRTDYNASVSVPDSSGPERILSISADIETIGEI LKKIIPTLEEGLQLPSPTATSQLPLESDAVECLNYQHYKGSDFDCELRLL IHQSLAGGIIGVKGAKIKELRENTQTTIKLFQECCPHSTDRVVLIGGKPD RVVECIKIILDLISESPIKGRAQPYDPNFYDETYDYGGFTMMFDDRRGRP VGFPMRGRGGFDRMPPGRGGRPMPPSRRDYDDMSPRRGPPPPPPGRGGRG GSRARNLPLPPPPPPRGGDLMAYDRRGRPGDRYDGMVGFSADETWDSAID TWSPSEWQMAYEPQGGSGYDYSYAGGRGSYGDLGGPIITTQVTIPKDLAG SIIGKGGQRIKQIRHESGASIKIDEPLEGSEDRIITITGTQDQIQNAQYL LQNSVKQYSGKFF |
| 预测分子量 | 77 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HNRNPK(异质核核糖核蛋白K)重组蛋白的3篇代表性文献，简要总结其内容：
1. **文献名称**：*hnRNP K: An HMG Box Protein in the c-myc Promoter*
**作者**：Takimoto M, et al.
**摘要**：该研究揭示了HNRNPK蛋白通过与c-myc基因启动子中的富嘧啶序列结合，调控c-myc转录的分子机制，并证明其作为DNA结合蛋白在肿瘤发生中的潜在作用。
2. **文献名称**：*hnRNP K interacts with RNA binding motif protein 42 and functions in the maintenance of cellular ATP level*
**作者**：Kim JH, et al.
**摘要**：研究发现HNRNPK通过与RBM42蛋白相互作用，参与细胞能量代谢调控，特别是通过维持ATP水平影响癌症细胞的增殖和存活，为靶向肿瘤代谢提供新思路。
3. **文献名称**：*Phosphorylation of hnRNP K by Cyclin-dependent Kinase 5 Affects Cargo Translation and Transport*
**作者**：Chen Y, et al.
**摘要**：该文献报道CDK5对HNRNPK的磷酸化修饰可调节其与mRNA的结合能力，进而影响神经元轴突中mRNA的运输和局部翻译，揭示了其在神经发育中的关键作用。
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**说明**：以上文献侧重HNRNPK在基因调控(癌症、代谢、神经)中的多功能性，重组蛋白研究常涉及结构解析(如结合位点)或功能验证(如突变体实验)。如需实验方法类文献或特定方向扩展，可进一步补充说明。
**Background of Recombinant HNRPK Protein**
Heterogeneous Nuclear Ribonucleoprotein K (HNRPK or hnRNP K) is a multifunctional RNA-binding protein involved in diverse cellular processes, including transcription, RNA splicing, translation, and signal transduction. It belongs to the hnRNP family, which plays critical roles in post-transcriptional gene regulation. HNRPK contains three K-homology (KH) domains that mediate interactions with nucleic acids and proteins, enabling its participation in complex molecular networks.
HNRPK regulates gene expression by binding to specific RNA/DNA sequences, such as C-rich motifs, and interacts with signaling molecules like p53. Src, and ERK. Its activity is modulated by post-translational modifications (e.g., phosphorylation, methylation), which influence subcellular localization and functional specificity. Dysregulation of HNRPK is linked to cancers, neurodegenerative diseases, and viral infections, underscoring its biomedical relevance.
Recombinant HNRPK protein is engineered using expression systems (e.g., *E. coli*, mammalian cells*) to produce purified, functional protein for research. Its recombinant form enables studies on molecular mechanisms, structural analysis, and screening for therapeutic agents targeting HNRPK-associated pathways. Researchers utilize it to explore roles in stress responses, cell cycle control, and disease pathology, making it a vital tool in molecular and translational biology.
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