| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RPL6 |
| Uniprot No | Q02878 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-288aa |
| 氨基酸序列 | MAGEKVEKPDTKEKKPEAKKVDAGGKVKKGNLKAKKPKKGKPHCSRNPVLVRGIGRYSRSAMYSRKAMYKRKYSAAKSKVEKKKKEKVLATVTKPVGGDKNGGTRVVKLRKMPRYYPTEDVPRKLLSHGKKPFSQHVRKLRASITPGTILIILTGRHRGKRVVFLKQLASGLLLVTGPLVLNRVPLRRTHQKFVIATSTKIDISNVKIPKHLTDAYFKKKKLRKPRHQEGEIFDTEKEKYEITEQRKIDQKAVDSQILPKIKAIPQLQGYLRSVFALTNGIYPHKLVF |
| 预测分子量 | 32,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RPL6重组蛋白的参考文献示例(注：以下内容为模拟示例，实际文献需通过学术数据库查询)：
1. **文献名称**：*"Expression and Purification of Recombinant Human RPL6 in Escherichia coli for Structural Studies"*
**作者**：Zhang Y. et al.
**摘要**：研究报道了通过原核表达系统(大肠杆菌)成功克隆、表达并纯化重组人源RPL6蛋白，利用亲和层析和尺寸排阻色谱优化纯化流程，为后续核糖体结构解析提供基础。
2. **文献名称**：*"RPL6 Promotes Tumorigenesis in Breast Cancer by Modulating Ribosome Biogenesis and Cell Proliferation"*
**作者**：Wang L. et al.
**摘要**：研究发现RPL6在乳腺癌细胞中过表达，重组RPL6蛋白的敲低实验表明其通过调控核糖体生物合成和细胞周期相关基因(如c-Myc)促进肿瘤生长和转移。
3. **文献名称**：*"Recombinant RPL6 Interacts with Hepatitis C Virus Core Protein and Facilitates Viral Replication"*
**作者**：Huang X. et al.
**摘要**：通过体外重组RPL6蛋白与丙型肝炎病毒(HCV)核心蛋白的互作研究，揭示了RPL6在病毒复制中的作用，为抗病毒治疗提供了潜在靶点。
4. **文献名称**：*"Structural Insights into the Role of RPL6 in Ribosome Assembly Using Cryo-EM"*
**作者**：Smith J. et al.
**摘要**：利用冷冻电镜技术解析了重组RPL6蛋白在核糖体大亚基组装中的构象变化，阐明了其与rRNA及其他核糖体蛋白的关键相互作用位点。
建议通过 **PubMed**、**Google Scholar** 或 **Web of Science** 输入关键词“RPL6 recombinant protein”或“RPL6 ribosome”获取最新文献。
**Background of Recombinant RPL6 Protein**
Recombinant RPL6 (Ribosomal Protein L6) is a engineered version of the human RPL6 protein, produced through genetic recombination techniques for research and therapeutic applications. RPL6 is a structural and functional component of the 60S ribosomal subunit, playing a critical role in ribosome biogenesis, protein synthesis, and the regulation of translation. As a member of the ribosomal protein family, it contributes to the assembly and stability of the ribosome, ensuring accurate mRNA decoding and peptide bond formation during translation.
The recombinant form of RPL6 is typically expressed in heterologous systems such as *E. coli*, yeast, or mammalian cells, enabling large-scale production with high purity. This involves cloning the RPL6 gene into expression vectors, followed by protein extraction and purification using affinity chromatography (e.g., His-tag or GST-tag systems). Recombinant RPL6 retains the biological activity of the native protein, including its ability to interact with rRNA and other ribosomal proteins, making it valuable for studying ribosome dynamics and translational regulation.
Structurally, RPL6 contains conserved domains involved in RNA binding and ribosomal assembly. Studies highlight its role in cellular stress responses, cell cycle regulation, and apoptosis, with dysregulation linked to diseases such as cancer. For instance, RPL6 overexpression has been observed in certain cancers, suggesting its potential as a biomarker or therapeutic target. Recombinant RPL6 is also utilized in structural biology (e.g., cryo-EM studies) to elucidate ribosome architecture and in drug screening to identify compounds targeting ribosome-related pathways.
Overall, recombinant RPL6 serves as a crucial tool for advancing our understanding of ribosome function, disease mechanisms, and the development of novel biomedical interventions.
×
