| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HSD17b3 |
| Uniprot No | P37058 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-310aa |
| 氨基酸序列 | MGDVLEQFFI LTGLLVCLAC LAKCVRFSRC VLLNYWKVLP KSFLRSMGQW AVITGAGDGI GKAYSFELAK RGLNVVLISR TLEKLEAIAT EIERTTGRSV KIIQADFTKD DIYEHIKEKL AGLEIGILVN NVGMLPNLLP SHFLNAPDEI QSLIHCNITS VVKMTQLILK HMESRQKGLI LNISSGIALF PWPLYSMYSA SKAFVCAFSK ALQEEYKAKE VIIQVLTPYA VSTAMTKYLN TNVITKTADE FVKESLNYVT IGGETCGCLA HEILAGFLSL IPAWAFYSGA FQRLLLTHYV AYLKLNTKVR |
| 预测分子量 | 34,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HSD17B3重组蛋白的3篇模拟参考文献及其摘要概括：
1. **文献名称**：*Expression and purification of recombinant human HSD17B3 in Escherichia coli for functional studies*
**作者**：Smith J, et al.
**摘要**：研究通过大肠杆菌系统高效表达并纯化HSD17B3重组蛋白，验证其催化雄烯二酮转化为睾酮的活性，为酶动力学研究提供基础。
2. **文献名称**：*Structural insights into HSD17B3 mutations causing 46.XY disorders of sexual development*
**作者**：Lee S, et al.
**摘要**：通过X射线晶体学解析HSD17B3重组蛋白结构，分析致病突变对酶活性及底物结合的影响，揭示其与性发育异常的分子机制。
3. **文献名称**：*Functional characterization of HSD17B3 isoforms using a mammalian cell overexpression system*
**作者**：Garcia R, et al.
**摘要**：在HEK293细胞中表达不同HSD17B3重组变体，发现特定剪接异构体活性缺失，提示其在睾酮合成障碍中的潜在作用。
注：以上文献为示例，实际引用需以真实发表的论文为准。建议通过PubMed或Google Scholar检索关键词“HSD17B3 recombinant protein”获取最新文献。
**Background of HSD17B3 Recombinant Protein**
HSD17B3 (17β-hydroxysteroid dehydrogenase type 3) is a key enzyme in steroidogenesis, primarily responsible for catalyzing the reduction of androstenedione to testosterone, a critical step in androgen biosynthesis. This NADPH-dependent enzyme belongs to the 17β-HSD family, which regulates the interconversion of active and inactive sex steroids. HSD17B3 is predominantly expressed in the testes, where it ensures proper testosterone production during male sexual development and maintenance of secondary sexual characteristics.
Mutations in the *HSD17B3* gene lead to 17β-HSD3 deficiency, a rare autosomal recessive disorder causing 46.XY differences in sex development (DSD). Affected individuals exhibit undervirilization due to impaired testosterone synthesis, often requiring clinical intervention. Recombinant HSD17B3 protein is engineered to study these mutations, enabling functional assays to diagnose enzyme deficiencies and evaluate pathogenicity.
The production of recombinant HSD17B3 involves cloning the human *HSD17B3* gene into expression systems (e.g., bacterial, insect, or mammalian cells) to yield purified, bioactive enzyme. This recombinant protein retains catalytic activity, allowing researchers to analyze substrate specificity, kinetic properties, and inhibitor interactions. Its applications extend to drug discovery, particularly in targeting androgen-related conditions like prostate cancer or disorders linked to steroid metabolism.
Beyond clinical research, recombinant HSD17B3 aids in elucidating the structural and mechanistic basis of steroidogenic pathways, offering insights into enzyme regulation and potential therapeutic strategies. Its role in modeling genetic variants also supports personalized medicine approaches for hormone-dependent diseases. Overall, HSD17B3 recombinant protein serves as a vital tool for advancing both basic science and translational studies in endocrinology and reproductive health.
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