| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DHEA |
| Uniprot No | P43403 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-619aa |
| 氨基酸序列 | MPDPAAHLPF FYGSISRAEA EEHLKLAGMA DGLFLLRQCL RSLGGYVLSL VHDVRFHHFP IERQLNGTYA IAGGKAHCGP AELCEFYSRD PDGLPCNLRK PCNRPSGLEP QPGVFDCLRD AMVRDYVRQT WKLEGEALEQ AIISQAPQVE KLIATTAHER MPWYHSSLTR EEAERKLYSG AQTDGKFLLR PRKEQGTYAL SLIYGKTVYH YLISQDKAGK YCIPEGTKFD TLWQLVEYLK LKADGLIYCL KEACPNSSAS NASGAAAPTL PAHPSTLTHP QRRIDTLNSD GYTPEPARIT SPDKPRPMPM DTSVYESPYS DPEELKDKKL FLKRDNLLIA DIELGCGNFG SVRQGVYRMR KKQIDVAIKV LKQGTEKADT EEMMREAQIM HQLDNPYIVR LIGVCQAEAL MLVMEMAGGG PLHKFLVGKR EEIPVSNVAE LLHQVSMGMK YLEEKNFVHR DLAARNVLLV NRHYAKISDF GLSKALGADD SYYTARSAGK WPLKWYAPEC INFRKFSSRS DVWSYGVTMW EALSYGQKPY KKMKGPEVMA FIEQGKRMEC PPECPPELYA LMSDCWIYKW EDRPDFLTVE QRMRACYYSL ASKVEGPPGS TQKAEAACA |
| 预测分子量 | 69,8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DHEA重组蛋白的3篇假设性参考文献示例，涵盖不同研究方向：
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1. **文献名称**: *"Heterologous Expression and Functional Characterization of Recombinant Human CYP17A1 for DHEA Biosynthesis"*
**作者**: Smith J., Brown K., Lee R.
**摘要**: 研究通过在大肠杆菌中重组表达人源CYP17A1酶，优化其催化孕烯醇酮转化为DHEA的条件，证实重组酶的高活性及稳定性，为规模化生产DHEA提供新方法。
2. **文献名称**: *"Engineering a Recombinant DHEA-Immunoglobulin Fusion Protein for Enhanced Stability and Immune Modulation"*
**作者**: Zhang Y., Wang L., Chen X.
**摘要**: 构建DHEA与IgG Fc片段的重组融合蛋白，显著延长其半衰期，并在小鼠模型中验证其对T细胞活化的调节作用，为免疫相关疾病治疗提供潜在策略。
3. **文献名称**: *"Structural Insights into Recombinant DHEA-Binding Protein Using Cryo-EM and Molecular Dynamics Simulations"*
**作者**: Johnson M.T., Patel S., Gupta A.
**摘要**: 通过冷冻电镜解析重组DHEA结合蛋白的三维结构，揭示其与DHEA相互作用的特异性位点，阐明其在激素信号转导中的调控机制。
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**说明**：上述文献为假设性示例，实际研究中DHEA重组蛋白相关文献可能更多聚焦于其合成酶(如CYP17A1)的重组表达或DHEA代谢相关蛋白的结构功能分析。建议通过PubMed或Web of Science以“recombinant CYP17A1”、“DHEA biosynthesis”等关键词检索最新文献。
**Background of DHEA Recombinant Protein**
Dehydroepiandrosterone (DHEA), a steroid hormone precursor primarily secreted by the adrenal glands, plays a pivotal role in synthesizing sex hormones (e.g., testosterone, estrogen) and regulating metabolic, immune, and neuroendocrine functions. Declining DHEA levels with age have been linked to age-related disorders, sparking interest in therapeutic applications. However, direct hormone supplementation faces challenges, including off-target effects and metabolic instability.
Recombinant protein technology offers an alternative approach to harness DHEA's benefits. DHEA recombinant proteins are engineered using genetic modification to express specific enzymes or receptors involved in DHEA synthesis or signaling. For instance, recombinant CYP17A1 (a cytochrome P450 enzyme critical for DHEA production) has been studied to modulate steroidogenesis. Similarly, recombinant proteins targeting DHEA-binding receptors, such as membrane-associated receptors or nuclear hormone receptors, aim to mimic or enhance DHEA's physiological effects without relying on exogenous hormone administration.
Research applications include studying DHEA's role in immune modulation, aging, and metabolic syndromes. Therapeutically, recombinant proteins could enable precise targeting of pathways linked to inflammation, insulin resistance, or neurodegeneration. Advances in expression systems (e.g., *E. coli*, yeast, mammalian cells) and protein engineering (e.g., fusion tags, stability enhancements) continue to optimize production and functionality. Challenges remain in ensuring bioavailability, tissue specificity, and minimizing immunogenicity. Nonetheless, DHEA recombinant proteins represent a promising strategy to translate DHEA's multifaceted biology into targeted therapies, bridging gaps between endocrine research and clinical innovation.
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