| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PGN |
| Uniprot No | Q9UQ90 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-795aa |
| 氨基酸序列 | MAVLLLLLRALRRGPGPGPRPLWGPGPAWSPGFPARPGRGRPYMASRPPGDLAEAGGRALQSLQLRLLTPTFEGINGLLLKQHLVQNPVRLWQLLGGTFYFNTSRLKQKNKEKDKSKGKAPEEDEEERRRRERDDQMYRERLRTLLVIAVVMSLLNALSTSGGSISWNDFVHEMLAKGEVQRVQVVPESDVVEVYLHPGAVVFGRPRLALMYRMQVANIDKFEEKLRAAEDELNIEAKDRIPVSYKRTGFFGNALYSVGMTAVGLAILWYVFRLAGMTGREGGFSAFNQLKMARFTIVDGKMGKGVSFKDVAGMHEAKLEVREFVDYLKSPERFLQLGAKVPKGALLLGPPGCGKTLLAKAVATEAQVPFLAMAGPEFVEVIGGLGAARVRSLFKEARARAPCIVYIDEIDAVGKKRSTTMSGFSNTEEEQTLNQLLVEMDGMGTTDHVIVLASTNRADILDGALMRPGRLDRHVFIDLPTLQERREIFEQHLKSLKLTQSSTFYSQRLAELTPGFSGADIANICNEAALHAAREGHTSVHTLNFEYAVERVLAGTAKKSKILSKEEQKVVAFHESGHALVGWMLEHTEAVMKVSITPRTNAALGFAQMLPRDQHLFTKEQLFERMCMALGGRASEALSFNEVTSGAQDDLRKVTRIAYSMVKQFGMAPGIGPISFPEAQEGLMGIGRRPFSQGLQQMMDHEARLLVAKAYRHTEKVLQDNLDKLQALANALLEKEVINYEDIEALIGPPPHGPKKMIAPQRWIDAQREKQDLGEEETEETQQPPLGGEEPTWPK |
| 预测分子量 | 88,2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于PGN(肽聚糖，Peptidoglycan)重组蛋白研究的示例参考文献(注：示例为假设性文献，实际文献需通过数据库检索确认)：
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1. **文献名称**: *"Recombinant production and functional characterization of a peptidoglycan hydrolase from bacteriophage for antibacterial applications"*
**作者**: Smith J, et al.
**摘要**: 本研究通过大肠杆菌表达系统重组表达了噬菌体来源的PGN水解酶(重组蛋白LysoPGN)，并验证其特异性裂解革兰氏阳性菌细胞壁PGN的能力。实验表明该蛋白在体外和动物模型中均表现出显著抑菌效果，为新型抗菌剂开发提供候选分子。
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2. **文献名称**: *"Structural analysis of a recombinant PGN-binding protein in host-pathogen interaction"*
**作者**: Zhang Y, et al.
**摘要**: 通过X射线晶体学解析了重组表达的宿主PGN识别蛋白(rPGBP)的三维结构，揭示了其与PGN中MurNAc残基结合的关键结构域。功能实验证实rPGBP通过调控TLR2信号通路参与先天免疫应答，为抗感染治疗提供新靶点。
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3. **文献名称**: *"Heterologous expression of a PGN synthase enzyme and its role in bacterial cell wall remodeling"*
**作者**: Kumar A, et al.
**摘要**: 在枯草芽孢杆菌中重组表达来自金黄色葡萄球菌的PGN合成酶(rPgnS)，发现其可修饰宿主PGN交联度并增强β-内酰胺类抗生素敏感性。该研究为通过重组技术干预PGN生物合成以增强传统抗生素疗效提供了依据。
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**注**：实际研究中可检索关键词如 **"recombinant peptidoglycan-associated protein"** 或 **"PGN-binding protein expression"**，并筛选涉及重组蛋白表达、PGN互作机制或应用(如抗菌、免疫调节)的文献。推荐使用PubMed、Web of Science等数据库获取最新权威研究。
**Background of PGN Recombinant Protein**
Recombinant proteins, engineered through genetic modification, have revolutionized biomedical research and therapeutic development. PGN (peptidoglycan) recombinant protein, a derivative of bacterial cell wall components, represents a specialized class of such proteins with unique applications. Peptidoglycan, a polymer of sugars and amino acids, is a critical structural element in bacterial cell walls, providing rigidity and protection. However, isolated native peptidoglycan often triggers strong immune responses, limiting its direct use. Recombinant PGN proteins address this by enabling controlled production of specific peptidoglycan-derived epitopes or functional domains.
The development of PGN recombinant proteins leverages recombinant DNA technology, where genes encoding target peptidoglycan fragments are cloned into expression vectors (e.g., *E. coli*, yeast, or mammalian systems). This allows scalable, high-purity production while minimizing endotoxin contamination. Advanced purification techniques, such as affinity chromatography, ensure structural integrity and biological activity.
PGN recombinant proteins are pivotal in studying host-pathogen interactions, particularly in innate immunity. They serve as ligands for pattern recognition receptors (e.g., NOD-like receptors), helping unravel signaling pathways in inflammatory diseases. Additionally, these proteins are explored as vaccine adjuvants or components, enhancing immune responses against bacterial infections. In diagnostics, they aid in detecting anti-peptidoglycan antibodies for conditions like autoimmune diseases.
Recent advances include engineering hybrid PGN proteins fused with antigenic epitopes for targeted immunotherapy. Challenges remain in optimizing stability and reducing immunogenicity for clinical use. Nonetheless, PGN recombinant proteins exemplify the synergy between microbial biology and biotechnology, offering tools for therapeutic innovation and mechanistic research. Their versatility continues to expand, driven by demands for precision in infectious disease management and immune modulation.
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