| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | AMPH |
| Uniprot No | P49418 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-695aa |
| 氨基酸序列 | MADIKTGIFAKNVQKRLNRAQEKVLQKLGKADETKDEQFEEYVQNFKRQEAEGTRLQRELRGYLAAIKGMQEASMKLTESLHEVYEPDWYGREDVKMVGEKCDVLWEDFHQKLVDGSLLTLDTYLGQFPDIKNRIAKRSRKLVDYDSARHHLEALQSSKRKDESRISKAEEEFQKAQKVFEEFNVDLQEELPSLWSRRVGFYVNTFKNVSSLEAKFHKEIAVLCHKLYEVMTKLGDQHADKAFTIQGAPSDSGPLRIAKTPSPPEEPSPLPSPTASPNHTLAPASPAPARPRSPSQTRKGPPVPPLPKVTPTKELQQENIISFFEDNFVPEISVTTPSQNEVPEVKKEETLLDLDFDPFKPEVTPAGSAGVTHSPMSQTLPWDLWTTSTDLVQPASGGSFNGFTQPQDTSLFTMQTDQSMICNLAESEQAPPTEPKAEEPLAAVTPAVGLDLGMDTRAEEPVEEAVIIPGADADAAVGTLVSAAEGAPGEEAEAEKATVPAGEGVSLEEAKIGTETTEGAESAQPEAEELEATVPQEKVIPSVVIEPASNHEEEGENEITIGAEPKETTEDAAPPGPTSETPELATEQKPIQDPQPTPSAPAMGAADQLASAREASQELPPGFLYKVETLHDFEAANSDELTLQRGDVVLVVPSDSEADQDAGWLVGVKESDWLQYRDLATYKGLFPENFTRRLD |
| 预测分子量 | 81.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于AMPH(amphiphysin)重组蛋白的3篇文献示例：
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1. **文献名称**: "Recombinant expression and functional characterization of amphiphysin in synaptic vesicle endocytosis"
**作者**: Smith A, et al.
**摘要**: 研究报道了AMPH重组蛋白在大肠杆菌中的高效表达与纯化，验证了其通过SH3结构域与动力酶(dynamin)的相互作用，并证明其在突触小泡内吞过程中的关键调控作用。
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2. **文献名称**: "Structural insights into amphiphysin-induced membrane curvature sensing"
**作者**: Lee B, et al.
**摘要**: 通过X射线晶体学解析了重组AMPH蛋白的BAR结构域结构，揭示了其通过二聚化感知并诱导膜曲率变化的分子机制，为神经细胞膜重塑研究提供了结构基础。
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3. **文献名称**: "Amphiphysin-mediated endocytosis in cancer cell proliferation"
**作者**: Chen X, et al.
**摘要**: 利用重组AMPH蛋白探究其在癌细胞中的异常表达，发现其通过调控受体内吞促进肿瘤细胞增殖，为靶向AMPH的癌症治疗策略提供了实验依据。
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注：以上文献为示例，实际引用时请根据具体研究方向补充真实文献信息。
AMPH (amphiphysin) is a multifunctional protein primarily involved in membrane curvature dynamics and clathrin-mediated endocytosis, particularly in neuronal synapses. It plays a critical role in synaptic vesicle recycling by interacting with clathrin, dynamin, and other endocytic machinery components. Structurally, AMPH contains an N-terminal amphipathic helix for membrane binding, a central coiled-coil domain for dimerization, and a C-terminal SH3 domain that recruits proline-rich motif-containing partners. Dysregulation of AMPH is linked to neurological disorders and cancer. Autoantibodies targeting AMPH are associated with paraneoplastic stiff-person syndrome and other autoimmune conditions. In cancer, AMPH overexpression has been observed in breast tumors and melanoma, where it may influence cell invasion and metastasis.
Recombinant AMPH proteins are engineered using expression systems like E. coli or mammalian cells to study its biochemical functions, structure-activity relationships, and disease mechanisms. These purified proteins serve as tools for investigating endocytic pathways, synaptic transmission, and pathological interactions in autoimmune or neoplastic contexts. Researchers utilize AMPH recombinant proteins in binding assays (e.g., with dynamin or clathrin), structural studies (cryo-EM/X-ray crystallography), and diagnostic applications (detecting autoimmune antibodies). Modified versions, including truncated mutants or tagged variants (His-tag, GFP fusion), enable domain-specific functional analysis and cellular localization tracking. Current therapeutic exploration includes developing AMPH-targeting biologics for autoimmune conditions and leveraging its role in membrane remodeling for drug delivery system design. The production of bioactive recombinant AMPH continues to advance through optimized purification protocols and quality control measures for functional consistency.
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