| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TRPV6 |
| Uniprot No | Q9H1D0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-765aa |
| 氨基酸序列 | MGPLQGDGGPALGGADVAPRLSPVRVWPRPQAPKEPALHPMGLSLPKEKGLILCLWSKFCRWFQRRESWAQSRDEQNLLQQKRIWESPLLLAAKDNDVQALNKLLKYEDCKVHQRGAMGETALHIAALYDNLEAAMVLMEAAPELVFEPMTSELYEGQTALHIAVVNQNMNLVRALLARRASVSARATGTAFRRSPCNLIYFGEHPLSFAACVNSEEIVRLLIEHGADIRAQDSLGNTVLHILILQPNKTFACQMYNLLLSYDRHGDHLQPLDLVPNHQGLTPFKLAGVEGNTVMFQHLMQKRKHTQWTYGPLTSTLYDLTEIDSSGDEQSLLELIITTKKREARQILDQTPVKELVSLKWKRYGRPYFCMLGAIYLLYIICFTMCCIYRPLKPRTNNRTSPRDNTLLQQKLLQEAYMTPKDDIRLVGELVTVIGAIIILLVEVPDIFRMGVTRFFGQTILGGPFHVLIITYAFMVLVTMVMRLISASGEVVPMSFALVLGWCNVMYFARGFQMLGPFTIMIQKMIFGDLMRFCWLMAVVILGFASAFYIIFQTEDPEELGHFYDYPMALFSTFELFLTIIDGPANYNVDLPFMYSITYAAFAIIATLLMLNLLIAMMGDTHWRVAHERDELWRAQIVATTVMLERKLPRCLWPRSGICGREYGLGDRWFLRVEDRQDLNRQRIQRYAQAFHTRGSEDLDKDSVEKLELGCPFSPHLSLPMPSVSRSTSRSSANWERLRQGTLRRDLRGIINRGLEDGESWEYQI |
| 预测分子量 | 88.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TRPV6重组蛋白的3篇代表性文献示例(注：文献为虚构，仅作格式参考，实际引用需核实真实来源)：
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1. **文献名称**: *Structural insights into the human TRPV6 channel by cryo-EM*
**作者**: Smith A, et al.
**摘要**: 通过冷冻电镜解析人源TRPV6重组蛋白的高分辨率结构，揭示其钙离子选择性渗透的分子机制，并探讨其胞内结构域在通道门控调控中的作用。
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2. **文献名称**: *Functional characterization of recombinant TRPV6 in cancer cell proliferation*
**作者**: Lee J, et al.
**摘要**: 在HEK293细胞中重组表达TRPV6蛋白，证明其过表达促进癌细胞钙内流，并通过MAPK信号通路增强细胞增殖，提示TRPV6可能成为癌症治疗靶点。
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3. **文献名称**: *Calcium-dependent regulation of TRPV6 reconstituted in lipid bilayers*
**作者**: Brown K, et al.
**摘要**: 利用脂质体重组TRPV6蛋白，结合电生理学实验，阐明胞内钙离子浓度通过结合EF-hand结构域负反馈调节通道活性的分子机制。
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如需真实文献，建议在PubMed或Web of Science中检索关键词“TRPV6 recombinant expression”或“TRPV6 purification”。
**Background of TRPV6 Recombinant Protein**
TRPV6 (Transient Receptor Potential Vanilloid 6) is a calcium-selective ion channel belonging to the TRP superfamily, which plays a critical role in cellular calcium homeostasis. Predominantly expressed in epithelial tissues such as the intestine, kidney, and placenta, TRPV6 facilitates calcium absorption and reabsorption, making it essential for maintaining systemic calcium balance, bone mineralization, and cellular signaling. Dysregulation of TRPV6 has been implicated in pathologies including hypercalciuria, kidney stones, osteoporosis, and certain cancers, particularly those with metastatic potential.
Recombinant TRPV6 protein is engineered for *in vitro* studies to elucidate its structure, function, and regulatory mechanisms. Produced using heterologous expression systems (e.g., HEK293 cells, insect cells, or yeast), the recombinant protein is often tagged with markers like His or FLAG for purification and detection. Structural studies reveal that TRPV6 forms a homotetrameric channel with six transmembrane domains per subunit, a pore-forming loop, and intracellular N- and C-terminal domains involved in calcium-dependent inactivation and modulation by calmodulin.
Research utilizing recombinant TRPV6 has advanced understanding of its gating mechanisms, ion selectivity, and interactions with pharmacological agents. For example, inhibitors such as 2-aminoethoxydiphenyl borate (2-APB) and natural compounds like curcumin have been tested for therapeutic potential. Additionally, recombinant TRPV6 serves as a tool to study genetic mutations linked to calcium dysregulation and to screen drugs targeting calcium-related disorders. Its role in cancer progression, particularly in prostate and breast cancers, underscores its relevance in oncology research.
Overall, TRPV6 recombinant protein provides a vital platform for dissecting calcium signaling pathways and developing targeted therapies for diseases associated with calcium mishandling.
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