| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SCRN1 |
| Uniprot No | Q12765 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-414aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMAAAPPSYCFVAFPPRAKDGLVVFGKN SARPRDEVQEVVYFSAADHEPESKVECTYISIDQVPRTYAIMISRPAWLW GAEMGANEHGVCIANEAINTREPAAEIEALLGMDLVRLGLERGETAKEAL DVIVSLLEEHGQGGNYFEDANSCHSFQSAYLIVDRDEAWVLETIGKYWAA EKVTEGVRCICSQLSLTTKMDAEHPELRSYAQSQGWWTGEGEFNFSEVFS PVEDHLDCGAGKDSLEKQEESITVQTMMNTLRDKASGVCIDSEFFLTTAS GVSVLPQNRSSPCIHYFTGTPDPSRSIFKPFIFVDDVKLVPKTQSPCFGD DDPAKKEPRFQEKPDRRHELYKAHEWARAIIESDQEQGRKLRSTMLELEK QGLEAMEEILTSSEPLDPAEVGDLFYDCVDTEIKFFK |
| 预测分子量 | 49 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于SCRN1重组蛋白的参考文献及其摘要概括：
1. **文献名称**: "Recombinant SCRN1 promotes secretory vesicle maturation via regulating Rab GTPase activity"
**作者**: Tanaka K. et al.
**摘要**: 研究利用大肠杆菌表达系统成功制备了SCRN1重组蛋白，发现其通过激活Rab3A GTP酶活性促进神经内分泌细胞分泌囊泡的成熟过程，揭示了SCRN1在胞吐作用中的分子机制。
2. **文献名称**: "Crystal structure of SCRN1 reveals a novel phospholipid-binding domain"
**作者**: Chen L. et al.
**摘要**: 首次解析了SCRN1重组蛋白的晶体结构，鉴定出其N端含有新型磷脂结合结构域，体外实验证实该结构域通过与细胞膜磷脂互作调控分泌颗粒锚定，为SCRN1功能研究提供结构基础。
3. **文献名称**: "SCRN1 recombinant protein attenuates Aβ toxicity in Alzheimer's disease models"
**作者**: Park J.H. et al.
**摘要**: 利用昆虫细胞系统表达SCRN1重组蛋白，证明其能通过增强自噬途径清除β淀粉样蛋白(Aβ)，显著改善阿尔茨海默病模型小鼠的认知功能障碍，提示潜在治疗应用价值。
注：上述文献为模拟案例，实际研究中建议通过PubMed或Web of Science以"SCRN1 recombinant"为关键词检索真实论文。
**Background of SCRN1 Recombinant Protein**
SCRN1 (Secernin-1) is a cytosolic protein encoded by the *SCRN1* gene, primarily implicated in regulating secretory processes and membrane trafficking. It belongs to the secernin family, which is evolutionarily conserved and involved in vesicle-mediated transport, particularly in exocytosis and endocytosis pathways. SCRN1 interacts with components of the SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) complex, playing a role in facilitating membrane fusion events critical for intracellular communication and cargo transport.
Studies suggest SCRN1 is enriched in secretory cells, such as neurons and endocrine cells, where it modulates the release of neurotransmitters and hormones. Its function extends to maintaining Golgi apparatus integrity and influencing autophagy, a cellular recycling mechanism. Dysregulation of SCRN1 has been linked to neurodegenerative diseases, cancer, and immune disorders, highlighting its broad physiological relevance. For instance, SCRN1 overexpression is observed in certain tumors, where it may promote cell proliferation and metastasis, while its depletion correlates with impaired secretory pathways in neurological conditions.
The recombinant SCRN1 protein is engineered using expression systems like *E. coli* or mammalian cells, ensuring high purity and bioactivity for experimental applications. It serves as a vital tool for studying SCRN1’s molecular interactions, structural properties, and mechanistic roles in cellular processes. Researchers employ it in assays such as protein-protein interaction studies, enzymatic activity analyses, and drug screening to explore therapeutic targeting of SCRN1-associated pathways.
Overall, SCRN1 recombinant protein bridges fundamental research and translational studies, offering insights into cellular dynamics and disease mechanisms while enabling the development of diagnostic or therapeutic strategies.
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