| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CAPN9 |
| Uniprot No | O14815 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-690aa |
| 氨基酸序列 | MPYLYRAPGP QAHPVPKDAR ITHSSGQSFE QMRQECLQRG TLFEDADFPA SNSSLFYSER PQIPFVWKRP GEIVKNPEFI LGGATRTDIC QGELGDCWLL AAIASLTLNQ KALARVIPQD QSFGPGYAGI FHFQFWQHSE WLDVVIDDRL PTFRDRLVFL HSADHNEFWS ALLEKAYAKL NGSYEALKGG SAIEAMEDFT GGVAETFQTK EAPENFYEIL EKALKRGSLL GCFIDTRSAA ESEARTPFGL IKGHAYSVTG IDQVSFRGQR IELIRIRNPW GQVEWNGSWS DSSPEWRSVG PAEQKRLCHT ALDDGEFWMA FKDFKAHFDK VEICNLTPDA LEEDAIHKWE VTVHQGSWVR GSTAGGCRNF LDTFWTNPQI KLSLTEKDEG QEECSFLVAL MQKDRRKLKR FGANVLTIGY AIYECPDKDE HLNKDFFRYH ASRARSKTFI NLREVSDRFK LPPGEYILIP STFEPHQEAD FCLRIFSEKK AITRDMDGNV DIDLPEPPKP TPPDQETEEE QRFRALFEQV AGEDMEVTAE ELEYVLNAVL QKKKDIKFKK LSLISCKNII SLMDTSGNGK LEFDEFKVFW DKLKQWINLF LRFDADKSGT MSTYELRTAL KAAGFQLSSH LLQLIVLRYA DEELQLDFDD FLNCLVRLEN ASRVFQALST KNKEFIHLNI NEFIHLTMNI |
| 预测分子量 | 79 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CAPN9重组蛋白的3篇示例参考文献(注：文献为模拟示例，非真实存在)：
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1. **标题**：Expression and Functional Characterization of Recombinant Human CAPN9 in Escherichia coli
**作者**：Li, X., Wang, Y., & Chen, J.
**摘要**：该研究成功在大肠杆菌中表达并纯化了重组人源CAPN9蛋白，优化了诱导条件以提高可溶性表达。通过体外酶活实验证实重组CAPN9具有钙依赖性蛋白酶活性，并发现其可特异性切割细胞骨架蛋白α-actinin，提示其在细胞迁移中的潜在作用。
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2. **标题**：CAPN9 Regulates Gastric Epithelial Homeostasis via Proteolytic Activation of Digestive Enzymes
**作者**：Tanaka, K., Suzuki, H., & Nakamura, R.
**摘要**：本研究揭示了CAPN9重组蛋白在胃黏膜中的功能，证明其通过切割胃蛋白酶原前体促进活性酶生成。实验表明，CAPN9缺失的小鼠出现消化功能异常，而外源性添加重组CAPN9可恢复酶活性，提示其在维持胃肠道稳态中的关键作用。
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3. **标题**：Structural Insights into CAPN9 Inhibition by Calpastatin: Implications for Cancer Therapy
**作者**：Zhang, L., et al.
**摘要**：通过冷冻电镜解析CAPN9与其天然抑制剂calpastatin的复合物结构，发现结合界面上的关键氨基酸残基。重组CAPN9的突变体实验表明，破坏该界面可增强蛋白酶活性，为靶向CAPN9的结直肠癌治疗策略提供结构基础。
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这些示例覆盖了重组CAPN9的表达纯化、生理功能及结构机制研究，符合该蛋白在消化系统与疾病领域的研究热点。实际文献需通过数据库(如PubMed)以“CAPN9 recombinant”或“CAPN9 protein”为关键词检索。
CAPN9. or calpain-9. is a member of the calpain family of calcium-dependent cysteine proteases, which play diverse roles in cellular processes such as signaling, apoptosis, and cytoskeletal remodeling. Unlike ubiquitously expressed calpains (e.g., calpain-1/2), CAPN9 exhibits tissue-specific expression, predominantly in the gastrointestinal tract, particularly in gastric epithelial cells. It consists of a conserved protease domain and calcium-binding regulatory domains, requiring calcium for activation. CAPN9 forms heterodimers with regulatory subunits like calpain-8 (CAPN8) to stabilize its enzymatic activity, a unique feature among calpains.
Functionally, CAPN9 is implicated in maintaining gastric mucosal integrity and digestive physiology. Studies suggest its involvement in cell differentiation, apoptosis, and mucosal defense against stressors like Helicobacter pylori infection. Notably, CAPN9 acts as a tumor suppressor in gastric cancer, where its downregulation correlates with disease progression. It may regulate pathways like Wnt/β-catenin and NF-κB, influencing cell proliferation and inflammation.
Recombinant CAPN9 protein is engineered using expression systems (e.g., E. coli, mammalian cells) to study its biochemical properties, substrate specificity, and interactions. Purification often involves affinity tags (e.g., His-tag) for high yield and purity. This tool enables exploration of CAPN9's role in gastrointestinal pathologies, drug screening for calpain inhibitors, and therapeutic strategies targeting gastric cancer. Its recombinant form also aids in elucidating calpain family redundancy and developing isoform-specific modulators. Despite progress, CAPN9's full mechanistic spectrum and therapeutic potential remain active research areas.
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