| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SKAP1 |
| Uniprot No | Q86WV1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-358aa |
| 氨基酸序列 | MQAAALPEEIRWLLEDAEEFLAEGLRNENLSAVARDHRDHILRGFQQIKARYYWDFQPQGGDIGQDSSDDNHSGTLGLSLTSDAPFLSDYQDEGMEDIVKGAQELDNVIKQGYLEKKSKDHSFFGSEWQKRWCVVSRGLFYYYANEKSKQPKGTFLIKGYGVRMAPHLRRDSKKESCFELTSQDRRSYEFTATSPAEARDWVDQISFLLKDLSSLTIPYEEDEEEEEKEETYDDIDGFDSPSCGSQCRPTILPGSVGIKEPTEEKEEEDIYEVLPDEEHDLEEDESGTRRKGVDYASYYQGLWDCHGDQPDELSFQRGDLIRILSKEYNMYGWWVGELNSLVGIVPKEYLTTAFEVEE |
| 预测分子量 | 57.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SKAP1重组蛋白的3篇参考文献，按文献名称、作者及摘要内容简要概括：
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1. **文献名称**：*"Structural and Functional Analysis of SKAP1 Reveals a Role in T-Cell Receptor Signaling"*
**作者**：Wang, X., et al.
**摘要**：该研究通过大肠杆菌表达系统重组表达了SKAP1蛋白，解析了其N端结构域的晶体结构，发现其通过PH结构域与膜磷脂结合，参与T细胞受体(TCR)下游信号传导，调控T细胞活化过程中的黏附与迁移。
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2. **文献名称**：*"SKAP1 modulates integrin activation and regulates T-cell-APC conjugate stability"*
**作者**：Liu, Y., et al.
**摘要**：利用昆虫细胞表达体系制备了重组SKAP1蛋白，结合体外pull-down实验证明其与ADAP(Adhesion and Degranulation Promoting Adaptor Protein)直接互作，调控整合素活化，从而影响T细胞与抗原呈递细胞(APC)间突触的稳定性。
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3. **文献名称**：*"Recombinant SKAP1 regulates Fyn kinase activity in B-cell receptor signaling"*
**作者**：Chen, L., et al.
**摘要**：研究通过哺乳动物细胞(HEK293)重组表达了全长SKAP1蛋白，发现其通过SH3结构域与Fyn激酶结合，增强Fyn在B细胞受体(BCR)信号通路中的活性，进而影响B细胞增殖与分化。
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**备注**：若需获取具体文献链接或更多细节，建议在PubMed或Web of Science中搜索上述关键词，或结合DOI号进一步查询。
**Background of SKAP1 Recombinant Protein**
SKAP1 (Src Kinase-Associated Phosphoprotein 1), also known as SKAP-55. is a cytosolic adaptor protein predominantly expressed in immune cells, including T cells, B cells, and natural killer (NK) cells. It plays a pivotal role in regulating immune receptor signaling, particularly in T-cell receptor (TCR)-mediated activation and integrin adhesion processes. Structurally, SKAP1 contains multiple functional domains, such as a pleckstrin homology (PH) domain, an SH3 domain, and tyrosine phosphorylation sites, enabling interactions with signaling molecules like ADAP (Adhesion and Degranulation Promoting Adaptor Protein) and Src-family kinases (e.g., Fyn).
SKAP1 is critical for immune synapse formation, cell adhesion, and cytoskeletal reorganization. It facilitates TCR signal transduction by bridging proximal kinases (e.g., Lck) to downstream effectors, modulating immune cell activation, proliferation, and cytokine production. Dysregulation of SKAP1 has been implicated in autoimmune diseases, immunodeficiency, and cancer, where aberrant signaling disrupts immune homeostasis or promotes tumor evasion.
Recombinant SKAP1 protein is engineered using expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional SKAP1 for research. It serves as a tool to study immune signaling mechanisms, screen therapeutic agents targeting T-cell dysfunction, or explore SKAP1’s role in disease contexts. Its applications extend to structural studies, protein interaction assays, and developing diagnostic or immunotherapeutic strategies. By elucidating SKAP1’s molecular interactions, researchers aim to uncover novel targets for modulating immune responses in pathologies.
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