| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CAPN5 |
| Uniprot No | O15484 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-640aa |
| 氨基酸序列 | MFSCVKPYED QNYSALRRDC RRRKVLFEDP LFPATDDSLY YKGTPGPAVR WKRPKGICED PRLFVDGISS HDLHQGQVGN CWFVAACSSL ASRESLWQKV IPDWKEQEWD PEKPNAYAGI FHFHFWRFGE WVDVVIDDRL PTVNNQLIYC HSNSRNEFWC ALVEKAYAKL AGCYQALDGG NTADALVDFT GGVSEPIDLT EGDFANDETK RNQLFERMLK VHSRGGLISA SIKAVTAADM EARLACGLVK GHAYAVTDVR KVRLGHGLLA FFKSEKLDMI RLRNPWGERE WNGPWSDTSE EWQKVSKSER EKMGVTVQDD GEFWMTFEDV CRYFTDIIKC RVINTSHLSI HKTWEEARLH GAWTLHEDPR QNRGGGCINH KDTFFQNPQY IFEVKKPEDE VLICIQQRPK RSTRREGKGE NLAIGFDIYK VEENRQYRMH SLQHKAASSI YINSRSVFLR TDQPEGRYVI IPTTFEPGHT GEFLLRVFTD VPSNCRELRL DEPPHTCWSS LCGYPQLVTQ VHVLGAAGLK DSPTGANSYV IIKCEGDKVR SAVQKGTSTP EYNVKGIFYR KKLSQPITVQ VWNHRVLKDE FLGQVHLKAD PDNLQALHTL HLRDRNSRQP SNLPGTVAVH ILSSTSLMAV |
| 预测分子量 | 73,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CAPN5重组蛋白的3篇参考文献概览：
1. **文献名称**：*Expression and Characterization of Recombinant CAPN5 Protein in a Baculovirus System*
**作者**：Monterrubio-López, G.P. et al.
**摘要**：该研究利用昆虫细胞表达系统成功表达并纯化了CAPN5重组蛋白，验证了其钙依赖性蛋白酶活性，并分析了其在不同病理模型中的潜在作用机制。
2. **文献名称**：*CAPN5 Mutations Cause Hyperactivation of Calpain-5 and Retinal Degeneration*
**作者**：Mahajan, M. et al.
**摘要**：研究发现CAPN5基因突变导致重组蛋白的异常自催化活性，揭示了其在自身炎症性视网膜病变(如ADNIV)中的致病机制，并通过体外实验验证了突变体蛋白的过度酶活性。
3. **文献名称**：*Structural Insights into CAPN5 Protease Activation and Substrate Specificity*
**作者**：Hollander, J.D. et al.
**摘要**：通过X射线晶体学解析了CAPN5重组蛋白的3D结构，阐明了其钙离子结合域和催化核心的构象变化，为开发靶向抑制剂提供了结构基础。
4. **文献名称**：*Functional Interaction of CAPN5 with Inflammatory Cytokines in Ocular Tissues*
**作者**：Johnson, K.L. et al.
**摘要**：研究利用重组CAPN5蛋白证实其通过切割特定信号分子(如IL-1β前体)参与炎症反应调控，提示其在眼内免疫微环境中的潜在作用。
注：以上文献为示例，实际引用需以真实发表的论文为准。建议通过PubMed或Web of Science以“CAPN5 recombinant”或“CAPN5 protein purification”为关键词检索最新研究。
**Background on CAPN5 Recombinant Protein**
CAPN5. or calpain-5. belongs to the calpain family of calcium-dependent cysteine proteases involved in diverse cellular processes, including signal transduction, apoptosis, and cytoskeletal remodeling. Unlike classical calpains (e.g., CAPN1/CAPN2), CAPN5 is categorized as a "non-classical" calpain due to its unique domain structure and regulatory mechanisms. It contains a conserved catalytic domain but lacks the calcium-binding penta-EF-hand motif found in many family members, suggesting alternative calcium-sensing mechanisms.
Recombinant CAPN5 protein is engineered for *in vitro* studies to elucidate its enzymatic activity, substrate specificity, and pathological roles. Produced via heterologous expression systems (e.g., *E. coli* or mammalian cells*), the protein is purified to homogeneity, enabling biochemical assays and structural analyses. Research highlights CAPN5's link to diseases, particularly *autosomal dominant neovascular inflammatory vitreoretinopathy (ADNIV)*, where gain-of-function mutations drive abnormal protease activity, leading to retinal inflammation and degeneration.
Studies using recombinant CAPN5 have revealed its role in cleaving substrates like cytoskeletal proteins and signaling molecules, implicating it in cellular stress responses and disease pathways. Its dysregulation is also explored in neurodegenerative and inflammatory conditions. By leveraging recombinant CAPN5. researchers aim to develop targeted inhibitors or modulators, offering therapeutic potential for CAPN5-associated disorders.
Overall, CAPN5 recombinant protein serves as a critical tool for unraveling the enzyme's biology and translating findings into clinical interventions.
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