| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | MPP3 |
| Uniprot No | P08254 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 101-477aa |
| 氨基酸序列 | MRTFPGIPKW RKTHLTYRIV NYTPDLPKDA VDSAVEKALK VWEEVTPLTF SRLYEGEADI MISFAVREHG DFYPFDGPGN VLAHAYAPGP GINGDAHFDD DEQWTKDTTG TNLFLVAAHE IGHSLGLFHS ANTEALMYPL YHSLTDLTRF RLSQDDINGI QSLYGPPPDS PETPLVPTEP VPPEPGTPAN CDPALSFDAV STLRGEILIF KDRHFWRKSL RKLEPELHLI SSFWPSLPSG VDAAYEVTSK DLVFIFKGNQ FWAIRGNEVR AGYPRGIHTL GFPPTVRKID AAISDKEKNK TYFFVEDKYW RFDEKRNSME PGFPKQIAED FPGIDSKIDA VFEEFGFFYF FTGSSQLEFD PNAKKVTHTL KSNSWLNC |
| 预测分子量 | 43 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于MPP3重组蛋白的3篇参考文献摘要：
1. **文献名称**: "Structural characterization of recombinant MPP3 reveals its role in apical protein transport"
**作者**: Müller et al. (2016)
**摘要**: 研究通过大肠杆菌表达系统成功纯化重组MPP3蛋白，结合X射线晶体学解析其结构，揭示其参与细胞顶端膜蛋白运输的分子机制。
2. **文献名称**: "Recombinant MPP3 interacts with the polarity complex Scribble in vitro"
**作者**: Zhang & Chen (2018)
**摘要**: 利用昆虫细胞表达系统制备功能性MPP3重组蛋白，通过Pull-down实验证实其与细胞极性蛋白Scribble的直接相互作用，为上皮细胞极性调控提供依据。
3. **文献名称**: "Expression optimization and functional analysis of MPP3 in planar cell polarity signaling"
**作者**: Gupta et al. (2020)
**摘要**: 通过哺乳动物HEK293细胞表达重组MPP3.优化纯化方案后验证其在小平面细胞极性信号通路中的作用，发现其磷酸化修饰影响Wnt信号传导效率。
注：以上文献信息为示例性质，实际研究中建议通过PubMed或Web of Science以"MPP3 recombinant protein"等关键词检索最新文献。若需具体论文，可提供更详细的研究方向进一步筛选。
**Background of MPP3 Recombinant Protein**
MPP3 (Membrane Palmitoylated Protein 3), also known as MAGUK p55 subfamily member 3. is a member of the membrane-associated guanylate kinase (MAGUK) protein family. These proteins are characterized by conserved domains, including PDZ, SH3. and guanylate kinase (GUK)-like domains, which facilitate protein-protein interactions and cellular scaffolding. MPP3 is primarily localized to cell membranes, particularly in epithelial and neuronal tissues, where it plays a critical role in maintaining cell polarity, junctional complexes, and signal transduction pathways.
The recombinant MPP3 protein is engineered through genetic cloning and expression systems (e.g., *E. coli* or mammalian cells) to produce a purified, functional form for experimental studies. Recombinant technology enables precise control over post-translational modifications, ensuring consistency in biochemical and structural analyses. MPP3’s PDZ domains are essential for binding to transmembrane proteins, such as receptors and ion channels, while its SH3 and GUK domains mediate interactions with cytosolic signaling molecules.
Research highlights MPP3’s involvement in developmental processes and disease mechanisms. It interacts with signaling pathways like Wnt and Hippo, influencing cell proliferation and tissue homeostasis. Dysregulation of MPP3 has been linked to cancers, neurodevelopmental disorders, and epithelial barrier dysfunction. For instance, aberrant MPP3 expression is observed in colorectal cancer, where it may modulate oncogenic signaling.
Recombinant MPP3 is widely used to study its structural dynamics, map interaction networks, and screen for therapeutic compounds targeting its domains. Its role in cellular scaffolding also makes it a candidate for understanding mechanisms underlying tissue integrity and disease progression. By leveraging recombinant MPP3. researchers aim to uncover novel insights into MAGUK protein biology and develop strategies to modulate pathological pathways associated with its dysfunction.
This protein’s versatility in vitro and in vivo underscores its importance as a tool for both basic research and translational applications.
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