| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TAP2 |
| Uniprot No | Q9UDX4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-400aa |
| 氨基酸序列 | MSGRVGDLSP KQAETLAKFR ENVQDVLPAL PNPDDYFLLR WLRARNFDLQ KSEALLRKYM EFRKTMDIDH ILDWQPPEVI QKYMPGGLCG YDRDGCPVWY DIIGPLDPKG LLFSVTKQDL LKTKMRDCER ILHECDLQTE RLGKKIETIV MIFDCEGLGL KHFWKPLVEV YQEFFGLLEE NYPETLKFML IVKATKLFPV GYNLMKPFLS EDTRRKIIVL GNNWKEGLLK LISPEELPAQ FGGTLTDPDG NPKCLTKINY GGEIPKSMYV RDQVKTQYEH SVQINRGSSH QVEYEILFPG CVLRWQFSSD GADIGFGVFL KTKMGERQRA GEMTDVLPSQ RYNAHMVPED GNLTCSEAGV YVLRFDNTYS FVHAKKVSFT VEVLLPDEGM QKYDKELTPV |
| 预测分子量 | 46 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条关于TAP2重组蛋白的参考文献概括：
1. **"Structural insights into the mechanism of ATP-binding cassette transporter TAP2"**
*作者：Sheppard, D. N. 等*
摘要：通过X射线晶体学解析重组人源TAP2蛋白的分子结构，揭示其ATP结合域构象变化及抗原肽转运的分子机制。
2. **"Functional characterization of recombinant TAP2 in antigen processing and MHC class I presentation"**
*作者：Neefjes, J. 等*
摘要：利用重组TAP2蛋白表达系统，验证其在抗原肽跨膜转运中的必要性，并证明其与TAP1的协同作用对MHC I类分子递呈抗原的关键影响。
3. **"TAP2 gene mutations alter recombinant protein stability and peptide transport efficiency"**
*作者：de la Salle, H. 等*
摘要：研究TAP2基因突变对重组蛋白功能的影响，发现特定突变导致蛋白稳定性下降及抗原肽转运缺陷，与免疫缺陷疾病相关。
4. **"Recombinant TAP2 as a therapeutic target for restoring immune recognition in tumors"**
*作者：Seliger, B. 等*
摘要：探讨肿瘤中TAP2表达缺失对免疫逃逸的作用，提出重组TAP2蛋白递送策略可增强肿瘤细胞MHC I表达，提升T细胞杀伤效果。
(注：以上文献为示例性概括，实际文献需根据具体研究查阅。)
TAP2 (Transporter Associated with Antigen Processing 2) is a critical component of the antigen presentation pathway in vertebrates, playing a pivotal role in adaptive immunity. It forms a heterodimeric complex with TAP1. constituting the TAP transporter embedded in the endoplasmic reticulum (ER) membrane. This complex mediates the ATP-dependent translocation of cytosolic antigenic peptides into the ER lumen, where they are loaded onto major histocompatibility complex class I (MHC-I) molecules. Proper peptide loading enables MHC-I to present these antigens to CD8+ T cells, initiating immune responses against infected or malignant cells.
The TAP2 protein features a multi-domain structure, including a transmembrane segment and nucleotide-binding domains (NBDs) that hydrolyze ATP to drive peptide transport. Genetic polymorphisms in TAP2 have been linked to altered immune susceptibility, including autoimmune disorders and impaired viral immunity. Recombinant TAP2 proteins are engineered using expression systems like mammalian or insect cells to preserve post-translational modifications and functional integrity. These recombinant variants serve as essential tools for studying antigen processing mechanisms, resolving structural details through techniques like cryo-EM, and developing immunotherapies targeting MHC-I presentation defects. Additionally, they facilitate drug screening for conditions involving TAP dysfunction, such as certain cancers that downregulate TAP to evade immune detection. Research on recombinant TAP2 continues to advance our understanding of immune regulation and therapeutic interventions.
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