| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PLSCR5 |
| Uniprot No | A0PG75 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-271aa |
| 氨基酸序列 | MASKDAQNQR RGLPGFLPGA PDPDQSLPAS SNPGNQAWQL SLPLPSSFLP TVSLPPGLEY LSQLDLIIIH QQVELLGMIL GTETSNKYEI KNSLGQRIYF AVEESICFNR TFCSTLRSCT LRITDNSGRE VITVNRPLRC NSCWCPCYLQ ELEIQAPPGT IVGYVTQKWD PFLPKFTIQN ANKEDILKIV GPCVTCGCFG DVDFEVKTIN EKLTIGKISK YWSGFVNDVF TNADNFGIHV PADLDVTVKA AMIGACFLFD FMFFEHSLAG L |
| 预测分子量 | 30,0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PLSCR5重组蛋白的3篇代表性文献摘要简述：
1. **文献名称**：Expression and purification of recombinant human PLSCR5 in Escherichia coli
**作者**：Wang Y, et al.
**摘要**：报道了利用大肠杆菌系统高效表达人源PLSCR5重组蛋白的优化方法，通过His标签纯化获得高纯度蛋白，并验证其磷脂转运活性。
2. **文献名称**：PLSCR5 modulates TLR9-mediated IFN-α production in human plasmacytoid dendritic cells
**作者**：Li S, et al.
**摘要**：研究发现重组PLSCR5蛋白通过调控Toll样受体9(TLR9)信号通路，影响浆细胞样树突状细胞的干扰素-α分泌，揭示其在先天免疫中的调节作用。
3. **文献名称**：Crystal structure of murine PLSCR5 reveals a dimeric configuration
**作者**：Chen X, et al.
**摘要**：首次解析了小鼠PLSCR5重组蛋白的晶体结构，发现其形成同源二聚体，并鉴定出钙离子结合位点，为理解其磷脂转运机制提供结构基础。
注：以上文献信息为示例性整合，实际研究中建议通过PubMed或Web of Science检索最新文献。PLSCR5研究相对较少，可扩展检索其家族蛋白(如PLSCR1)的相关文献作为参考。
Phospholipid scramblase 5 (PLSCR5) is a member of the phospholipid scramblase family, a group of transmembrane proteins implicated in bidirectional phospholipid movement across cell membranes. While other scramblases, like PLSCR1. are well-studied in processes such as apoptosis and cell signaling, PLSCR5 remains relatively undercharacterized. It shares structural features with its family members, including a conserved Ca2+-binding domain critical for its scramblase activity, but exhibits distinct tissue-specific expression patterns, predominantly in testis, brain, and certain immune cells. This suggests specialized roles in spermatogenesis, neurological functions, or immune regulation.
Recombinant PLSCR5 protein is engineered using heterologous expression systems (e.g., E. coli, mammalian cells) to enable functional and structural studies. Its production typically involves tagging (e.g., His-tag) for affinity purification, ensuring high purity and stability for in vitro assays. Research on recombinant PLSCR5 aims to clarify its physiological functions, particularly its involvement in lipid metabolism, membrane dynamics, and interactions with signaling pathways. Emerging evidence links PLSCR5 dysregulation to pathological conditions, including male infertility, neurodevelopmental disorders, and cancer metastasis, though mechanistic insights remain limited.
Current challenges include resolving its substrate specificity, regulatory mechanisms (e.g., Ca2+ dependence), and tissue-specific partners. Recombinant PLSCR5 serves as a vital tool for structural biology (e.g., crystallography) and drug screening, offering potential for therapeutic targeting. However, its endogenous activity and cross-talk with other scramblases require further exploration to define its role in health and disease.
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