| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HMGCS2 |
| Uniprot No | P54868 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-508aa |
| 氨基酸序列 | MQRLLTPVKRILQLTRAVQETSLTPARLLPVAHQRFSTASAVPLAKTDTW PKDVGILALEVYFPAQYVDQTDLEKYNNVEAGKYTVGLGQTRMGFCSVQE DINSLCLTVVQRLMERIQLPWDSVGRLEVGTETIIDKSKAVKTVLMELFQ DSGNTDIEGIDTTNACYGGTASLFNAANWMESSSWDGRYAMVVCGDIAVY PSGNARPTGGAGAVAMLIGPKAPLALERGLRGTHMENVYDFYKPNLASEY PIVDGKLSIQCYLRALDRCYTSYRKKIQNQWKQAGSDRPFTLDDLQYMIF HTPFCKMVQKSLARLMFNDFLSASSDTQTSLYKGLEAFGGLKLEDTYTNK DLDKALLKASQDMFDKKTKASLYLSTHNGNMYTSSLYGCLASLLSHHSAQ ELAGSRIGAFSYGSGLAASFFSFRVSQDAAPGSPLDKLVSSTSDLPKRLA SRKCVSPEEFTEIMNQREQFYHKVNFSPPGDTNSLFPGTWYLERVDEQHR RKYARRPV |
| 预测分子量 | 82 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HMGCS2重组蛋白的3篇代表性文献示例(注：以下内容为模拟虚构，仅供参考格式)：
1. **文献名称**: *Expression and Purification of Recombinant Human HMGCS2 in Escherichia coli*
**作者**: Zhang L, et al.
**摘要**: 研究报道了人源HMGCS2基因在大肠杆菌中的高效表达及纯化方法，通过优化诱导条件获得可溶性重组蛋白，并验证其酶活性，为酮体代谢研究提供工具。
2. **文献名称**: *Structural Insights into HMGCS2 Catalytic Mechanism by Cryo-EM Analysis*
**作者**: Thompson R, et al.
**摘要**: 利用冷冻电镜解析了重组HMGCS2蛋白的三维结构，揭示了其底物结合位点和催化中心的关键氨基酸残基，阐明其在线粒体酮生成中的作用机制。
3. **文献名称**: *Functional Characterization of Recombinant HMGCS2 in Hepatic Lipid Metabolism*
**作者**: Kim S, et al.
**摘要**: 在HEK293细胞中表达重组HMGCS2.证实其通过调控乙酰辅酶A代谢影响肝脏脂质氧化和酮体生成，为代谢综合征研究提供分子依据。
**提示**：实际引用时建议通过PubMed或Google Scholar检索真实文献(关键词：HMGCS2 recombinant protein)，并优先选择近5年高影响力期刊文章。
**Background of HMGCS2 Recombinant Protein**
HMGCS2 (3-hydroxy-3-methylglutaryl-CoA synthase 2) is a mitochondrial enzyme critical for ketogenesis, a metabolic pathway that produces ketone bodies during periods of fasting or carbohydrate restriction. It catalyzes the condensation of acetoacetyl-CoA and acetyl-CoA to form HMG-CoA, a key intermediate in ketone body synthesis. HMGCS2 is predominantly expressed in the liver and plays a vital role in energy homeostasis, particularly in providing alternative energy sources (e.g., β-hydroxybutyrate) for peripheral tissues when glucose availability is limited. Dysregulation of HMGCS2 has been linked to metabolic disorders, including diabetes, obesity, and rare genetic conditions affecting ketogenesis.
Recombinant HMGCS2 protein is engineered through molecular cloning, often expressed in *E. coli* or mammalian cell systems to ensure proper folding and post-translational modifications. Its production enables detailed biochemical and functional studies, such as enzymatic activity assays, structural analysis (e.g., X-ray crystallography), and investigations into regulatory mechanisms (e.g., modulation by phosphorylation or sirtuins). Recombinant HMGCS2 also serves as a tool for drug discovery, targeting metabolic diseases or exploring therapeutic strategies to modulate ketogenesis.
Research on HMGCS2 has expanded into cancer biology, as some tumors exploit ketone metabolism for growth under low-nutrient conditions. Additionally, its role in aging and neurodegenerative diseases, where ketone bodies are neuroprotective, highlights its broad biomedical relevance. The availability of recombinant HMGCS2 facilitates these studies, offering insights into mitochondrial metabolism and potential clinical applications.
In summary, HMGCS2 recombinant protein is a valuable resource for understanding metabolic regulation, disease mechanisms, and therapeutic development, bridging basic science and translational research.
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