| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FEz2 |
| Uniprot No | Q9UHY8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-353aa |
| 氨基酸序列 | MAADGDWQDF YEFQEPARSL LDQENCNASP EPGAEAGAEA GGGADGFPAP ACSLEEKLSL CFRPSDPGAE PPRTAVRPIT ERSLLQGDEI WNALTDNYGN VMPVDWKSSH TRTLHLLTLN LSEKGVSDSL LFDTSDDEEL REQLDMHSII VSCVNDEPLF TADQVIEEIE EMMQESPDPE DDETPTQSDR LSMLSQEIQT LKRSSTGSYE ERVKRLSVSE LNEILEEIET AIKEYSEELV QQLALRDELE FEKEVKNSFI SVLIEVQNKQ KEHKETAKKK KKLKNGSSQN GKNERSHMPG TYLTTVIPYE KKNGPPSVED LQILTKILRA MKEDSEKVPS LLTDYILKVL CPT |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是模拟生成的关于FEZ2重组蛋白的参考文献示例(注：部分内容基于领域知识整合，可能不对应真实文献)：
---
1. **文献名称**：*Structural and Functional Analysis of FEZ2 as a Novel Axon Guidance Factor*
**作者**：T. Katada et al.
**摘要**：本研究解析了重组人FEZ2蛋白的晶体结构，证实其通过N端卷曲螺旋结构域与UNC5D受体相互作用，调控神经元轴突导向。体外实验表明FEZ2重组蛋白可诱导神经突生长方向改变。
2. **文献名称**：*Epigenetic Silencing of FEZ2 in Gastric Cancer and Its Tumor-Suppressive Role*
**作者**：K. Ishiguro et al.
**摘要**：通过重组FEZ2蛋白功能回复实验，发现其能抑制胃癌细胞迁移和侵袭。研究揭示了FEZ2启动子区高甲基化导致表达缺失，重组FEZ2可通过调控Wnt/β-catenin通路发挥抑癌作用。
3. **文献名称**：*FEZ2 Recombinant Protein Modulates Autophagy via LC3 Interaction*
**作者**：A. Sánchez et al.
**摘要**：利用大肠杆菌表达系统纯化FEZ2重组蛋白，发现其通过C端结构域直接结合LC3蛋白，促进自噬小体形成。该机制可能在神经退行性疾病模型中具有保护作用。
---
**注**：以上文献为示例性质，实际研究中请通过PubMed、Web of Science等平台检索真实文献。FEZ2相关研究多集中于神经发育、癌症及表观遗传调控领域，建议结合具体研究方向筛选文献。
FEz2. also known as Fasciculation and Elongation Protein Zeta-2. is a mammalian homolog of the *Caenorhabditis elegans* UNC-76 protein. It plays critical roles in neuronal development, particularly in axonal outgrowth, vesicle transport, and synaptic function. The FEz2 protein contains coiled-coil domains that mediate interactions with molecular motors like kinesin-1. facilitating intracellular cargo transport along microtubules. Its function is closely linked to the structurally similar FEz1 protein, though they exhibit distinct expression patterns and regulatory mechanisms.
Recombinant FEz2 protein is engineered using expression systems such as *E. coli* or mammalian cells to study its biochemical properties and interactions. Researchers often clone the FEz2 gene into plasmid vectors with affinity tags (e.g., His-tag or GST-tag) for simplified purification. This recombinant tool enables in vitro studies on FEz2’s role in neuronal signaling, including its binding partners (e.g., DISC1. a schizophrenia-associated protein) and its modulation of microtubule dynamics.
Dysregulation of FEz2 has been implicated in neuropsychiatric disorders, cancer, and neurodegenerative diseases. For example, altered FEz2 expression correlates with disrupted neural connectivity in schizophrenia and aggressive tumor phenotypes. Recombinant FEz2 is pivotal in screening therapeutic compounds targeting these pathways. Its study also contributes to understanding evolutionary conservation of axonal guidance mechanisms across species. Despite progress, questions remain about FEz2’s post-translational modifications and tissue-specific functions, driving ongoing research using recombinant protein-based approaches.
×
