| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PUF60 |
| Uniprot No | Q9UHX1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-559aa |
| 氨基酸序列 | MATATIALQV NGQQGGGSEP AAAAAVVAAG DKWKPPQGTD SIKMENGQST AAKLGLPPLT PEQQEALQKA KKYAMEQSIK SVLVKQTIAH QQQQLTNLQM AAVTMGFGDP LSPLQSMAAQ RQRALAIMCR VYVGSIYYEL GEDTIRQAFA PFGPIKSIDM SWDSVTMKHK GFAFVEYEVP EAAQLALEQM NSVMLGGRNI KVGRPSNIGQ AQPIIDQLAE EARAFNRIYV ASVHQDLSDD DIKSVFEAFG KIKSCTLARD PTTGKHKGYG FIEYEKAQSS QDAVSSMNLF DLGGQYLRVG KAVTPPMPLL TPATPGGLPP AAAVAAAAAT AKITAQEAVA GAAVLGTLGT PGLVSPALTL AQPLGTLPQA VMAAQAPGVI TGVTPARPPI PVTIPSVGVV NPILASPPTL GLLEPKKEKE EEELFPESER PEMLSEQEHM SISGSSARHM VMQKLLRKQE STVMVLRNMV DPKDIDDDLE GEVTEECGKF GAVNRVIIYQ EKQGEEEDAE IIVKIFVEFS IASETHKAIQ ALNGRWFAGR KVVAEVYDQE RFDNSDLSA |
| 预测分子量 | 59,8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PUF60重组蛋白的参考文献示例(注：部分信息为示例性概括，实际文献需通过学术数据库验证)：
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1. **"PUF60 regulates alternative splicing and transcriptional control in human cells"**
- **作者**: Page-McCaw, P.S., et al.
- **摘要**: 本研究通过重组PUF60蛋白的体外实验，揭示了其在RNA剪接中的关键作用。研究发现，PUF60与U2AF35协同调控选择性剪接事件，并通过结合特定RNA序列影响基因表达模式。
2. **"PUF60 deficiency leads to developmental defects via disrupted splicing in zebrafish"**
- **作者**: Huang, L., et al.
- **摘要**: 利用重组PUF60蛋白进行功能挽救实验，证明PUF60缺失导致斑马鱼胚胎发育异常。研究通过质谱分析发现PUF60与剪接体复合物相互作用，提示其在胚胎发育中的必要性。
3. **"Structural insights into PUF60-RNA interactions by X-ray crystallography"**
- **作者**: Kim, H.J., & Lee, J.Y.
- **摘要**: 通过重组表达并纯化人源PUF60蛋白，解析其与RNA结合的晶体结构，揭示了保守的RNA结合域(RRM)的关键氨基酸残基，为理解其分子机制提供结构基础。
4. **"Oncogenic role of PUF60 in colorectal cancer via mRNA stability regulation"**
- **作者**: Zhang, R., et al.
- **摘要**: 研究利用重组PUF60蛋白进行体外RNA结合实验，发现其通过稳定促癌基因mRNA(如MYC)促进结直肠癌进展，为靶向PUF60的癌症治疗提供依据。
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**备注**：以上文献标题及摘要内容为示例性质，实际研究请通过**PubMed**、**Google Scholar**等平台检索关键词“PUF60 recombinant”或结合具体研究领域筛选。如需精确文献，建议提供更具体的研究方向(如疾病模型、分子机制等)。
PUF60 (Poly(U)-binding-splicing factor 60 kDa) is a multifunctional RNA-binding protein encoded by the PUF60 gene in humans. Initially identified as a component of spliceosomes, it plays dual roles in pre-mRNA splicing and transcriptional regulation. Structurally, PUF60 contains two RNA recognition motifs (RRMs) and a PUFF domain, enabling interactions with uridine-rich RNA sequences and protein partners like the U2AF heterodimer. It functions as a splicing factor by facilitating recognition of weak or suboptimal splice sites, particularly in alternative splicing events, and cooperates with other splicing regulators to fine-tune mRNA diversity.
Beyond splicing, PUF60 participates in transcriptional control by interacting with DNA-bound activators (e.g., c-Fos) and co-activators (e.g., Med23), modulating RNA polymerase II activity. It also engages in SUMOylation pathways through associations with SUMO-conjugating enzymes, influencing protein localization and stability. Notably, PUF60 haploinsufficiency is linked to developmental disorders such as Verheij syndrome, characterized by intellectual disability, coloboma, and skeletal anomalies, highlighting its importance in embryogenesis.
Recombinant PUF60 proteins, typically produced in bacterial (E. coli) or mammalian expression systems, retain RNA-binding and protein-interaction capabilities. These engineered variants are indispensable tools for studying RNA processing mechanisms, protein-RNA/DNA interactions, and disease-associated mutations. In cancer research, recombinant PUF60 helps elucidate its paradoxical roles as both tumor suppressor (modulating apoptosis) and oncogenic collaborator (enhancing spliceosome activity in malignancies). Current studies also explore its involvement in neurodegeneration and viral RNA processing, making it a versatile target for therapeutic development.
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