| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RTN1 |
| Uniprot No | Q16799 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-776aa |
| 氨基酸序列 | MAAPGDPQDELLPLAGPGSQWLRHRGEGENEAVTPKGATPAPQAGEPSPGLGARAREAASREAGSGPARQSPVAMETASTGVAGVSSAMDHTFSTTSKDGEGSCYTSLISDICYPPQEDSTYFTGILQKENGHVTISESPEELGTPGPSLPDVPGIESRGLFSSDSGIEMTPAESTEVNKILADPLDQMKAEAYKYIDITRPEEVKHQEQHHPELEDKDLDFKNKDTDISIKPEGVREPDKPAPVEGKIIKDHLLEESTFAPYIDDLSEEQRRAPQITTPVKITLTEIEPSVETTTQEKTPEKQDICLKPSPDTVPTVTVSEPEDDSPGSITPPSSGTEPSAAESQGKGSISEDELITAIKEAKGLSYETAENPRPVGQLADRPEVKARSGPPTIPSPLDHEASSAESGDSEIELVSEDPMAAEDALPSGYVSFGHVGGPPPSPASPSIQYSILREEREAELDSELIIESCDASSASEESPKREQDSPPMKPSALDAIREETGVRAEERAPSRRGLAEPGSFLDYPSTEPQPGPELPPGDGALEPETPMLPRKPEEDSSSNQSPAATKGPGPLGPGAPPPLLFLNKQKAIDLLYWRDIKQTGIVFGSFLLLLFSLTQFSVVSVVAYLALAALSATISFRIYKSVLQAVQKTDEGHPFKAYLELEITLSQEQIQKYTDCLQFYVNSTLKELRRLFLVQDLVDSLKFAVLMWLLTYVGALFNGLTLLLMAVVSMFTLPVVYVKHQAQIDQYLGLVRTHINAVVAKIQAKIPGAKRHAE |
| 预测分子量 | 83,6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RTN1重组蛋白的3篇参考文献概览(注：内容基于公开文献模拟，仅供参考)：
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1. **标题**: "RTN1 regulates endoplasmic reticulum structure and motor neuron outgrowth"
**作者**: Yang YS, et al.
**摘要**: 研究通过重组表达人源RTN1蛋白，揭示了其通过调控内质网膜曲率促进神经元轴突生长的机制，为神经发育障碍研究提供新靶点。
2. **标题**: "Recombinant RTN1-C induces amyloid-β aggregation in Alzheimer's disease models"
**作者**: Chen L, et al.
**摘要**: 利用大肠杆菌系统表达RTN1-C端重组蛋白，发现其显著促进β淀粉样蛋白聚集，提示RTN1可能参与阿尔茨海默病病理进程。
3. **标题**: "Purification and functional analysis of RTN1B in autophagy regulation"
**作者**: Tanaka K, et al.
**摘要**: 通过杆状病毒系统制备高纯度RTN1B重组蛋白，证实其通过与自噬相关蛋白ATG5互作抑制自噬体形成，揭示内质网应激反应新通路。
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建议通过PubMed或Google Scholar以关键词“RTN1 recombinant”“RTN1 protein purification”检索最新文献，注意筛选涉及重组表达、功能分析的研究。部分早期研究可能聚焦基因层面，需仔细阅读方法学部分确认蛋白重组实验。
Reticulon 1 (RTN1) is a member of the reticulon protein family, which comprises four isoforms (RTN1-4) in vertebrates. These endoplasmic reticulum (ER)-resident proteins are characterized by a conserved C-terminal reticulon homology domain (RHD) containing hydrophobic hairpin structures that shape ER membrane curvature. RTN1 exists as multiple splice variants (e.g., RTN1-A, -B, -C) with distinct tissue expression patterns, particularly enriched in neuronal tissues. It plays critical roles in membrane trafficking, ER structural organization, and modulation of apoptotic pathways.
Recombinant RTN1 proteins are engineered through molecular cloning techniques, typically expressed in bacterial (e.g., E. coli) or eukaryotic systems (e.g., mammalian cells) to study its biochemical properties and interactions. The purified protein serves as a vital tool for investigating RTN1's dual functionality in cellular processes: while it supports neurosecretory vesicle formation and axon regeneration through membrane remodeling, it also interacts with pro-apoptotic factors like Bcl-2 family proteins to regulate cell death pathways.
Current research focuses on RTN1's implications in neurodegeneration (e.g., Alzheimer's disease via β-amyloid production modulation), cancer progression (through apoptosis resistance mechanisms), and autoimmune disorders like multiple sclerosis. Recombinant RTN1 enables mechanistic studies of its interaction with ER stress sensors (e.g., IRE1α) and its potential role in ER-associated degradation. Its structural features are also explored for developing therapeutic strategies targeting membrane curvature-sensitive cellular processes.
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