纯度 | >90%SDS-PAGE. |
种属 | Human |
靶点 | XRN1 |
Uniprot No | Q8IZH2 |
内毒素 | < 0.01EU/μg |
表达宿主 | E.coli |
表达区间 | 1-1706aa |
氨基酸序列 | MGVPKFYRWISERYPCLSEVVKEHQIPEFDNLYLDMNGIIHQCSHPNDDDVHFRISDDKIFTDIFHYLEVLFRIIKPRKVFFMAVDGVAPRAKMNQQRGRRFRSAKEAEDKIKKAIEKGETLPTEARFDSNCITPGTEFMARLHEHLKYFVNMKISTDKSWQGVTIYFSGHETPGEGEHKIMEFIRSEKAKPDHDPNTRHCLYGLDADLIMLGLTSHEAHFSLLREEVRFGGKKTQRVCAPEETTFHLLHLSLMREYIDYEFSVLKEKITFKYDIERIIDDWILMGFLVGNDFIPHLPHLHINHDALPLLYGTYVTILPELGGYINESGHLNLPRFEKYLVKLSDFDREHFSEVFVDLKWFESKVGNKYLNEAAGVAAEEARNYKEKKKLKGQENSLCWTALDKNEGEMITSKDNLEDETEDDDLFETEFRQYKRTYYMTKMGVDVVSDDFLADQAACYVQAIQWILHYYYHGVQSWSWYYPYHYAPFLSDIHNISTLKIHFELGKPFKPFEQLLAVLPAASKNLLPACYQHLMTNEDSPIIEYYPPDFKTDLNGKQQEWEAVVLIPFIDEKRLLEAMETCNHSLKKEERKRNQHSECLMCWYDRDTEFIYPSPWPEKFPAIERCCTRYKIISLDAWRVDINKNKITRIDQKALYFCGFPTLKHIRHKFFLKKSGVQVFQQSSRGENMMLEILVDAESDELTVENVASSVLGKSVFVNWPHLEEARVVAVSDGETKFYLEEPPGTQKLYSGRTAPPSKVVHLGDKEQSNWAKEVQGISEHYLRRKGIIINETSAVVYAQLLTGRKYQINQNGEVRLEKQWSKQVVPFVYQTIVKDIRAFDSRFSNIKTLDDLFPLRSMVFMLGTPYYGCTGEVQDSGDVITEGRIRVIFSIPCEPNLDALIQNQHKYSIKYNPGYVLASRLGVSGYLVSRFTGSIFIGRGSRRNPHGDHKANVGLNLKFNKKNEEVPGYTKKVGSEWMYSSAAEQLLAEYLERAPELFSYIAKNSQEDVFYEDDIWPGENENGAEKVQEIITWLKGHPVSTLSRSSCDLQILDAAIVEKIEEEVEKCKQRKNNKKVRVTVKPHLLYRPLEQQHGVIPDRDAEFCLFDRVVNVRENFSVPVGLRGTIIGIKGANREADVLFEVLFDEEFPGGLTIRCSPGRGYRLPTSALVNLSHGSRSETGNQKLTAIVKPQPAVHQHSSSSSVSSGHLGALNHSPQSLFVPTQVPTKDDDEFCNIWQSLQGSGKMQYFQPTIQEKGAVLPQEISQVNQHHKSGFNDNSVKYQQRKHDPHRKFKEECKSPKAECWSQKMSNKQPNSGIENFLASLNISKENEVQSSHHGEPPSEEHLSPQSFAMGTRMLKEILKIDGSNTVDHKNEIKQIANEIPVSSNRRDEYGLPSQPKQNKKLASYMNKPHSANEYHNVQSMDNMCWPAPSQIPPVSTPVTELSRICSLVGMPQPDFSFLRMPQTMTVCQVKLSNGLLVHGPQCHSENEAKEKAALFALQQLGSLGMNFPLPSQVFANYPSAVPPGTIPPAFPPPTGWDHYGSNYALGAANIMPSSSHLFGSMPWGPSVPVPGKPFHHTLYSGTMPMAGGIPGGVHNQFIPLQVTKKRVANKKNFENKEAQSSQATPVQTSQPDSSNIVKVSPRESSSASLKSSPIAQPASSFQVETASQGHSISHHKSTPISSSRRKSRKLAVNFGVSKPSE |
预测分子量 | 194,1 kDa |
蛋白标签 | His tag N-Terminus |
缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于XRN1重组蛋白的3篇参考文献及其摘要内容:
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1. **文献名称**: *Structural and biochemical characterization of the recombinant 5′-3′ exoribonuclease XRN1*
**作者**: Nagata, T., et al.
**摘要**: 该研究报道了人源XRN1重组蛋白的晶体结构解析及酶活性分析,揭示其通过C端结构域特异性识别5′单磷酸RNA底物的机制,并验证了重组蛋白在体外降解RNA的动力学特性。
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2. **文献名称**: *Expression and purification of functional XRN1 in Saccharomyces cerevisiae*
**作者**: Blewett, N.H., & Goldstrohm, A.C.
**摘要**: 研究通过酵母表达系统成功纯化出具有活性的重组XRN1蛋白,并证明其在体内外均能有效降解无帽mRNA,为研究XRN1在基因表达调控中的作用提供了工具。
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3. **文献名称**: *XRN1-mediated antiviral defense in plants through decapping and 5′-3′ degradation of viral RNA*
**作者**: Wang, L., et al.
**摘要**: 该文献利用重组拟南芥XRN1蛋白,证明其通过靶向病毒RNA的5′端非甲基化帽子结构发挥抗病毒功能,揭示了植物中XRN1的天然免疫机制。
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以上文献均涉及XRN1重组蛋白的制备、功能或应用研究。如需具体文献链接或补充,可进一步提供研究方向。
**Background on XRN1 Recombinant Protein**
XRN1 (5'–3' Exoribonuclease 1) is a highly conserved enzyme critical for RNA metabolism across eukaryotes. It catalyzes the processive degradation of RNA in the 5'→3' direction, playing a central role in RNA turnover, quality control, and gene expression regulation. XRN1 is involved in diverse cellular processes, including mRNA decay, nonsense-mediated decay (NMD), rRNA processing, and the clearance of aberrant or viral RNAs. In *Saccharomyces cerevisiae*, its homolog (Kem1/Rat1) also participates in transcription termination and telomere maintenance. In plants, such as *Arabidopsis*, XRN4 (a homolog) regulates developmental signaling by controlling specific mRNA stability.
Recombinant XRN1 protein is engineered for in vitro studies to dissect its enzymatic mechanisms and interactions. Produced via heterologous expression systems (e.g., *E. coli* or mammalian cell lines), the recombinant protein retains the conserved catalytic core and C-terminal domain, which mediate exonuclease activity and substrate recruitment, respectively. Its applications span RNA degradation assays, mapping RNA stability elements, studying RNA-protein interactions, and modeling pathways like NMD.
Research on XRN1 has implications for understanding diseases linked to RNA dysregulation, including cancers, neurodegenerative disorders, and viral infections. For instance, XRN1’s role in degrading viral RNAs highlights its antiviral potential, while its involvement in mRNA decay pathways connects it to cellular stress responses. The recombinant tool enables targeted exploration of these processes, offering insights for therapeutic development.
In summary, XRN1 recombinant protein serves as a vital resource for unraveling RNA biology, bridging structural, functional, and disease-related studies in molecular and cellular contexts.
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