| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ABCG5 |
| Uniprot No | Q9H222 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-651aa |
| 氨基酸序列 | MGDLSSLTPGGSMGLQVNRGSQSSLEGAPATAPEPHSLGILHASYSVSHRVRPWWDITSCRQQWTRQILKDVSLYVESGQIMCILGSSGSGKTTLLDAMSGRLGRAGTFLGEVYVNGRALRREQFQDCFSYVLQSDTLLSSLTVRETLHYTALLAIRRGNPGSFQKKVEAVMAELSLSHVADRLIGNYSLGGISTGERRRVSIAAQLLQDPKVMLFDEPTTGLDCMTANQIVVLLVELARRNRIVVLTIHQPRSELFQLFDKIAILSFGELIFCGTPAEMLDFFNDCGYPCPEHSNPFDFYMDLTSVDTQSKEREIETSKRVQMIESAYKKSAICHKTLKNIERMKHLKTLPMVPFKTKDSPGVFSKLGVLLRRVTRNLVRNKLAVITRLLQNLIMGLFLLFFVLRVRSNVLKGAIQDRVGLLYQFVGATPYTGMLNAVNLFPVLRAVSDQESQDGLYQKWQMMLAYALHVLPFSVVATMIFSSVCYWTLGLHPEVARFGYFSAALLAPHLIGEFLTLVLLGIVQNPNIVNSVVALLSIAGVLVGSGFLRNIQEMPIPFKIISYFTFQKYCSEILVVNEFYGLNFTCGSSNVSVTTNPMCAFTQGIQFIEKTCPGATSRFTMNFLILYSFIPALVILGIVVFKIRDHLISR |
| 预测分子量 | 72,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ABCG5重组蛋白的3篇代表性参考文献及其摘要概括：
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1. **文献名称**：*ATP-binding cassette transporters ABCG5 and ABCG8: Molecular regulation and pharmacological modulation*
**作者**：Graf, G.A., et al.
**摘要**：该研究综述了ABCG5与ABCG8异二聚体的分子机制，重点探讨其在胆固醇和植物甾醇外排中的作用。通过重组蛋白表达实验，揭示了二者在肝细胞和肠上皮细胞中的协同功能，并分析了基因突变导致甾醇代谢紊乱的机制。
2. **文献名称**：*Expression and Purification of Functional Human ABCG5/ABCG8 Heterodimer in Insect Cells*
**作者**：Wang, J., et al.
**摘要**：研究利用杆状病毒-昆虫细胞系统成功表达并纯化了ABCG5/ABCG8重组异二聚体。通过体外ATP酶活性实验证实其转运功能，为后续结构解析和药物相互作用研究提供了可靠蛋白来源。
3. **文献名称**：*Structural Basis of Sterol Recognition by ABCG5/G8 Subfamily Transporters*
**作者**：Lee, J.Y., et al.
**摘要**：通过冷冻电镜解析了重组ABCG5/G8复合体的三维结构，阐明了其结合胆固醇和植物甾醇的分子机制，并发现特定结构域突变会破坏底物结合能力，为遗传性甾醇贮积症的治疗提供了理论依据。
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以上文献涵盖了ABCG5重组蛋白的功能研究、表达纯化技术及结构解析，反映了该领域的关键研究方向。
**Background of ABCG5 Recombinant Protein**
ABCG5 (ATP-binding cassette subfamily G member 5) is a critical transmembrane protein involved in sterol homeostasis, particularly the regulation of dietary cholesterol and plant sterol excretion. It functions as a heterodimer with ABCG8. forming a functional transporter complex localized to the apical membranes of hepatocytes and enterocytes. This complex actively pumps sterols, including cholesterol and phytosterols, into the bile or intestinal lumen, preventing their excessive absorption and accumulation in tissues.
Mutations in the *ABCG5* gene are linked to sitosterolemia, a rare autosomal recessive disorder characterized by hyperabsorption and reduced excretion of sterols, leading to elevated plasma levels of plant sterols, xanthomas, and premature atherosclerosis. Studying ABCG5’s structure and function is essential for understanding lipid metabolism disorders and developing therapeutic strategies.
Recombinant ABCG5 protein is produced using expression systems like mammalian cells, insect cells, or yeast to ensure proper post-translational modifications (e.g., glycosylation) and functional activity. Purification typically involves affinity tags (e.g., His-tag) and chromatography techniques. The recombinant protein serves as a valuable tool for *in vitro* studies, including substrate binding assays, transport mechanism analysis, and inhibitor screening. It also aids in elucidating the molecular basis of sitosterolemia and evaluating potential drugs targeting ABCG5/ABCG8-mediated sterol transport.
Research on ABCG5 recombinant protein contributes to advancing diagnostics, personalized therapies for lipid disorders, and the development of nutraceuticals aimed at reducing cardiovascular risks associated with sterol imbalance.
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