| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PTPRN2 |
| Uniprot No | Q92932 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 22-615aa |
| 氨基酸序列 | AAPSSVPRGRQLPGRLGCLLEEGLCGASEACVNDGVFGRCQKVPAMDFYRYEVSPVALQRLRVALQKLSGTGFTWQDDYTQYVMDQELADLPKTYLRRPEASSPARPSKHSVGSERRYSREGGAALANALRRHLPFLEALSQAPASDVLARTHTAQDRPPAEGDDRFSESILTYVAHTSALTYPPGSRTQLREDLLPRTLGQLQPDELSPKVDSGVDRHHLMAALSAYAAQRPPAPPGEGSLEPQYLLRAPSRMPRPLLAPAAPQKWPSPLGDSEDPSSTGDGARIHTLLKDLQRQPAEVRGLSGLELDGMAELMAGLMQGVDHGVARGSPGRAALGESGEQADGPKATLRGDSFPDDGVQDDDDRLYQEVHRLSATLGGLLQDHGSRLLPGALPFARPLDMERKKSEHPESSLSSEEETAGVENVKSQTYSKDLLGQQPHSEPGAAAFGELQNQMPGPSKEEQSLPAGAQEALSDGLQLEVQPSEEEARGYIVTDRDPLRPEEGRRLVEDVARLLQVPSSAFADVEVLGPAVTFKVSANVQNVTTEDVEKATVDNKDKLEETSGLKILQTGVGSKSKLKFLPPQAEQEDSTKF |
| 预测分子量 | 66.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是几篇与PTPRN2重组蛋白相关的模拟参考文献(注：以下内容为示例性模拟文献，实际文献需通过学术数据库检索)：
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1. **标题**: *Cloning and Expression of Human PTPRN2 in E. coli for Autoantibody Detection*
**作者**: Smith J, et al.
**摘要**: 本研究成功克隆了人源PTPRN2基因，并在大肠杆菌中表达重组蛋白。通过Western blot验证其抗原性，证实该重组蛋白可用于1型糖尿病患者血清中IA-2β自身抗体的检测。
2. **标题**: *Structural Analysis of PTPRN2 Phosphatase Domain by Recombinant Protein Crystallography*
**作者**: Lee H, et al.
**摘要**: 利用重组PTPRN2蛋白的磷酸酶结构域进行晶体结构解析，揭示了其催化活性位点的构象特征，为理解其在胰岛素分泌和神经突触中的功能提供结构基础。
3. **标题**: *PTPRN2 Recombinant Protein as a Biomarker in Neurodegenerative Disorders*
**作者**: Garcia R, et al.
**摘要**: 通过表达纯化PTPRN2胞外域重组蛋白，发现其在阿尔茨海默病患者脑脊液中存在异常结合蛋白，提示其可能参与tau蛋白磷酸化调控通路。
4. **标题**: *Immunogenicity of PTPRN2 Recombinant Fragments in Autoimmune Diabetes Models*
**作者**: Wang X, et al.
**摘要**: 构建了PTPRN2不同片段的重组蛋白，并在NOD小鼠模型中评估其免疫原性，发现C端区域可诱导特异性T细胞反应，为糖尿病疫苗开发提供潜在靶点。
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建议通过PubMed、Google Scholar等平台检索真实文献，关键词：**PTPRN2 recombinant protein**, **IA-2β autoantibody**, **protein tyrosine phosphatase diabetes**。
**Background of PTPRN2 Recombinant Protein**
PTPRN2 (Protein Tyrosine Phosphatase Receptor Type N2), also known as IA-2β, is a member of the protein tyrosine phosphatase (PTP) family. This transmembrane glycoprotein is predominantly expressed in neuroendocrine tissues, including pancreatic islets, the brain, and adrenal glands. Structurally, PTPRN2 features an extracellular domain with homology to cell adhesion molecules, a single transmembrane region, and an intracellular catalytic domain. However, its enzymatic activity remains debated, as some studies suggest it may function as a receptor or adhesion molecule rather than a classical phosphatase.
Recombinant PTPRN2 protein is engineered in vitro using expression systems (e.g., *E. coli* or mammalian cells*) to produce purified, functional protein for research. This tool enables the study of PTPRN2’s role in neuroendocrine regulation, particularly in insulin secretion and synaptic vesicle trafficking. Dysregulation of PTPRN2 has been linked to type 1 diabetes, where it serves as an autoantigen, as well as neurological disorders and cancers. Recombinant forms are crucial for investigating its interactions with ligands, antibodies, or intracellular signaling partners.
Research applications include elucidating its contribution to diabetes pathogenesis, developing diagnostic assays for autoantibody detection, and exploring therapeutic strategies targeting neuroendocrine pathways. Despite progress, the precise mechanisms of PTPRN2 in health and disease remain understudied, highlighting the need for further exploration using recombinant protein-based approaches.
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