| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PLCd3 |
| Uniprot No | Q8N3E9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-789aa |
| 氨基酸序列 | MLCGRWRRCRRPPEEPPVAAQVAAQVAAPVALPSPPTPSDGGTKRPGLRALKKMGLTEDEDVRAMLRGSRLRKIRSRTWHKERLYRLQEDGLSVWFQRRIPRAPSQHIFFVQHIEAVREGHQSEGLRRFGGAFAPARCLTIAFKGRRKNLDLAAPTAEEAQRWVRGLTKLRARLDAMSQRERLDHWIHSYLHRADSNQDSKMSFKEIKSLLRMVNVDMNDMYAYLLFKECDHSNNDRLEGAEIEEFLRRLLKRPELEEIFHQYSGEDRVLSAPELLEFLEDQGEEGATLARAQQLIQTYELNETAKQHELMTLDGFMMYLLSPEGAALDNTHTCVFQDMNQPLAHYFISSSHNTYLTDSQIGGPSSTEAYVRAFAQGCRCVELDCWEGPGGEPVIYHGHTLTSKILFRDVVQAVRDHAFTLSPYPVILSLENHCGLEQQAAMARHLCTILGDMLVTQALDSPNPEELPSPEQLKGRVLVKGKKLPAARSEDGRALSDREEEEEDDEEEEEEVEAAAQRRLAKQISPELSALAVYCHATRLRTLHPAPNAPQPCQVSSLSERKAKKLIREAGNSFVRHNARQLTRVYPLGLRMNSANYSPQEMWNSGCQLVALNFQTPGYEMDLNAGRFLVNGQCGYVLKPACLRQPDSTFDPEYPGPPRTTLSIQVLTAQQLPKLNAEKPHSIVDPLVRIEIHGVPADCARQETDYVLNNGFNPRWGQTLQFQLRAPELALVRFVVEDYDATSPNDFVGQFTLPLSSLKQGYRHIHLLSKDGASLSPATLFIQIRIQRS |
| 预测分子量 | 94.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PLCD3重组蛋白的3篇参考文献的简要列举：
1. **文献名称**：*"Recombinant Phospholipase C Delta 3 (PLCD3) Expression and Its Role in Cancer Cell Apoptosis"*
**作者**：Chen, L. et al.
**摘要**：研究利用大肠杆菌系统成功表达并纯化PLCD3重组蛋白，证实其在体外促进癌细胞凋亡的功能，并揭示了PLCD3通过调控IP3信号通路抑制肿瘤生长的机制。
2. **文献名称**：*"Structural and Functional Analysis of Human PLCD3: Insights from Recombinant Protein Crystallography"*
**作者**：Kim, S. et al.
**摘要**：通过昆虫细胞表达系统获得高纯度PLCD3重组蛋白，解析其晶体结构，发现其催化结构域的特殊构象可能影响底物结合效率，为靶向药物设计提供依据。
3. **文献名称**：*"PLCD3 Regulates Neuronal Signal Transduction via Recombinant Protein-Mediated PIP2 Hydrolysis Assays"*
**作者**：Wang, Y. et al.
**摘要**：利用哺乳动物细胞表达重组PLCD3蛋白，验证其在神经元中特异性水解PIP2的能力，并发现其功能缺失可能导致神经突触传递异常。
(注：以上文献信息为模拟生成，实际引用时需以真实文献为准。)
**Background of PLCδ3 Recombinant Protein**
Phospholipase C delta 3 (PLCδ3), a member of the phospholipase C (PLC) enzyme family, plays a critical role in intracellular signaling by hydrolyzing phosphatidylinositol 4.5-bisphosphate (PIP₂) into two secondary messengers: inositol 1.4.5-trisphosphate (IP₃) and diacylglycerol (DAG). These molecules regulate calcium release from endoplasmic reticulum stores and protein kinase C (PKC) activation, respectively, influencing processes like cell proliferation, differentiation, and apoptosis. The PLCδ3 isoform is distinguished by its structure, containing pleckstrin homology (PH), EF-hand, and C2 domains, which mediate membrane interactions, calcium sensitivity, and subcellular localization.
Recombinant PLCδ3 protein is engineered using genetic cloning techniques, where the PLCδ3 gene is expressed in heterologous systems like *E. coli* or mammalian cell lines. This approach ensures high purity and activity, enabling researchers to study its enzymatic properties, structure-function relationships, and interactions with regulatory molecules (e.g., G-proteins or lipids). Its recombinant form often includes affinity tags (e.g., His-tag) for simplified purification and detection.
Studies utilizing PLCδ3 recombinant protein have advanced understanding of its role in diseases. For instance, aberrant PLCδ3 expression is linked to cancer progression, neurological disorders, and cardiovascular conditions. In research, the protein serves as a tool for screening modulators of PLC activity, aiding drug discovery. Additionally, it helps elucidate tissue-specific signaling mechanisms, as PLCδ3 is highly expressed in brain, liver, and reproductive tissues.
Overall, PLCδ3 recombinant protein is vital for dissecting phosphoinositide signaling pathways and developing therapeutic strategies targeting PLC-related pathologies. Its applications span basic biochemistry, molecular pharmacology, and translational medicine.
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