| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PLCb4 |
| Uniprot No | Q15147 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1175aa |
| 氨基酸序列 | MAKPYEFNWQKEVPSFLQEGAVFDRYEEESFVFEPNCLFKVDEFGFFLTWRSEGKEGQVLECSLINSIRSGAIPKDPKILAALEAVGKSENDLEGRIVCVCSGTDLVNISFTYMVAENPEVTKQWVEGLRSIIHNFRANNVSPMTCLKKHWMKLAFMTNTNGKIPVRSITRTFASGKTEKVIFQALKELGLPSGKNDEIEPTAFSYEKFYELTQKICPRTDIEDLFKKINGDKTDYLTVDQLVSFLNEHQRDPRLNEILFPFYDAKRAMQIIEMYEPDEDLKKKGLISSDGFCRYLMSDENAPVFLDRLELYQEMDHPLAHYFISSSHNTYLTGRQFGGKSSVEMYRQVLLAGCRCVELDCWDGKGEDQEPIITHGKAMCTDILFKDVIQAIKETAFVTSEYPVILSFENHCSKYQQYKMSKYCEDLFGDLLLKQALESHPLEPGRALPSPNDLKRKILIKNKRLKPEVEKKQLEALRSMMEAGESASPANILEDDNEEEIESADQEEEAHPEFKFGNELSADDLGHKEAVANSVKKGLVTVEDEQAWMASYKYVGATTNIHPYLSTMINYAQPVKFQGFHVAEERNIHYNMSSFNESVGLGYLKTHAIEFVNYNKRQMSRIYPKGGRVDSSNYMPQIFWNAGCQMVSLNYQTPDLAMQLNQGKFEYNGSCGYLLKPDFMRRPDRTFDPFSETPVDGVIAATCSVQVISGQFLSDKKIGTYVEVDMYGLPTDTIRKEFRTRMVMNNGLNPVYNEESFVFRKVILPDLAVLRIAVYDDNNKLIGQRILPLDGLQAGYRHISLRNEGNKPLSLPTIFCNIVLKTYVPDGFGDIVDALSDPKKFLSITEKRADQMRAMGIETSDIADVPSDTSKNDKKGKANTAKANVTPQSSSELRPTTTAALASGVEAKKGIELIPQVRIEDLKQMKAYLKHLKKQQKELNSLKKKHAKEHSTMQKLHCTQVDKIVAQYDKEKSTHEKILEKAMKKKGGSNCLEMKKETEIKIQTLTSDHKSKVKEIVAQHTKEWSEMINTHSAEEQEIRDLHLSQQCELLKKLLINAHEQQTQQLKLSHDRESKEMRAHQAKISMENSKAISQDKSIKNKAERERRVRELNSSNTKKFLEERKRLAMKQSKEMDQLKKVQLEHLEFLEKQNEQAKEMQQMVKLEAEMDRRPATVV |
| 预测分子量 | 134,4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PLCβ4重组蛋白的3篇参考文献示例(内容为虚构演示，仅用于格式参考)：
1. **文献名称**: "Expression and Purification of Recombinant PLCβ4 in Insect Cells"
**作者**: Smith A, et al.
**摘要**: 该研究报道了利用杆状病毒-昆虫细胞系统高效表达PLCβ4重组蛋白，并通过亲和层析纯化获得高活性蛋白，为后续酶学分析提供基础。
2. **文献名称**: "Structural Insights into PLCβ4 Activation by G Protein Subunits"
**作者**: Lee H, et al.
**摘要**: 通过晶体学解析重组PLCβ4与Gβγ复合物的三维结构，揭示了G蛋白调控PLCβ4催化结构域变构激活的分子机制。
3. **文献名称**: "Functional Characterization of PLCβ4 Isoforms in Neuronal Signaling"
**作者**: Tanaka K, et al.
**摘要**: 研究利用重组人源PLCβ4不同剪接变体，发现其在小脑浦肯野细胞中特异性介导代谢型谷氨酸受体信号通路的钙响应。
注：以上文献信息为模拟生成，实际引用时请以真实文献为准。建议通过PubMed或Web of Science检索关键词(如"recombinant PLCβ4"、"phospholipase C beta4 expression")获取准确文献。
**Background of PLCβ4 Recombinant Protein**
Phospholipase C beta 4 (PLCβ4) is a member of the phospholipase C (PLC) family, a group of enzymes critical for intracellular signal transduction. PLC enzymes catalyze the hydrolysis of phosphatidylinositol 4.5-bisphosphate (PIP₂) into two secondary messengers: inositol 1.4.5-trisphosphate (IP₃) and diacylglycerol (DAG). These molecules regulate diverse cellular processes, including calcium signaling, protein kinase C (PKC) activation, and gene expression. Among PLC isoforms, PLCβ4 is specifically activated by Gq-coupled receptors, linking extracellular signals to intracellular responses via G-protein-mediated pathways.
PLCβ4 is predominantly expressed in the brain, retina, and certain peripheral tissues, where it plays roles in neurotransmission, phototransduction, and cellular differentiation. Structurally, it contains conserved domains such as the pleckstrin homology (PH) domain, EF-hand motifs, and catalytic X/Y regions, which enable lipid binding, calcium sensitivity, and enzymatic activity. Dysregulation of PLCβ4 has been implicated in neurological disorders, vision defects, and cancer, highlighting its physiological and pathological relevance.
Recombinant PLCβ4 protein is produced using biotechnological systems (e.g., *E. coli* or mammalian cell cultures*) to ensure high purity and functionality. It serves as a vital tool for *in vitro* studies, enabling researchers to dissect Gq-mediated signaling mechanisms, screen potential therapeutics, or investigate mutations linked to diseases. Advanced purification techniques, such as affinity tags (e.g., His-tag) and chromatography, guarantee its stability and activity for experimental applications.
Overall, PLCβ4 recombinant protein bridges molecular research and therapeutic development, offering insights into cellular signaling complexities and disease mechanisms. Its study continues to advance understanding of PLC-driven pathways and their implications in health and disease.
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