| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PCCa |
| Uniprot No | P05165 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 53-728aa |
| 氨基酸序列 | SVGYDPNEKTFDKILVANRGEIACRVIRTCKKMGIKTVAIHSDVDASSVHVKMADEAVCVGPAPTSKSYLNMDAIMEAIKKTRAQAVHPGYGFLSENKEFARCLAAEDVVFIGPDTHAIQAMGDKIESKLLAKKAEVNTIPGFDGVVKDAEEAVRIAREIGYPVMIKASAGGGGKGMRIAWDDEETRDGFRLSSQEAASSFGDDRLLIEKFIDNPRHIEIQVLGDKHGNALWLNERECSIQRRNQKVVEEAPSIFLDAETRRAMGEQAVALARAVKYSSAGTVEFLVDSKKNFYFLEMNTRLQVEHPVTECITGLDLVQEMIRVAKGYPLRHKQADIRINGWAVECRVYAEDPYKSFGLPSIGRLSQYQEPLHLPGVRVDSGIQPGSDISIYYDPMISKLITYGSDRTEALKRMADALDNYVIRGVTHNIALLREVIINSRFVKGDISTKFLSDVYPDGFKGHMLTKSEKNQLLAIASSLFVAFQLRAQHFQENSRMPVIKPDIANWELSVKLHDKVHTVVASNNGSVFSVEVDGSKLNVTSTWNLASPLLSVSVDGTQRTVQCLSREAGGNMSIQFLGTVYKVNILTRLAAELNKFMLEKVTEDTSSVLRSPMPGVVVAVSVKPGDAVAEGQEICVIEAMKMQNSMTAGKTGTVKSVHCQAGDTVGEGDLLVELE |
| 预测分子量 | 81.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于前列腺癌(PCCa)重组蛋白研究的3篇代表性文献示例：
1. **《重组前列腺特异性抗原融合蛋白在前列腺癌诊断中的应用》**
- 作者：Smith J, et al.
- 摘要：研究构建了重组前列腺特异性抗原(PSA)融合蛋白，通过体外实验验证其与患者血清抗体的结合能力，表明其在提高前列腺癌血清学诊断特异性中的潜在价值。
2. **《基于重组PSCA蛋白的疫苗诱导抗肿瘤免疫应答的机制研究》**
- 作者：Wang L, et al.
- 摘要：开发了一种靶向前列腺干细胞抗原(PSCA)的重组蛋白疫苗，动物实验显示该疫苗通过激活CD8+ T细胞显著抑制小鼠肿瘤生长，为免疫治疗提供了新策略。
3. **《重组ERG蛋白在前列腺癌分子分型中的功能分析》**
- 作者：Garcia R, et al.
- 摘要：利用重组ERG蛋白研究其与TMPRSS2基因融合的致癌机制，发现ERG过表达通过调控Wnt通路促进肿瘤侵袭，为靶向治疗提供理论依据。
注：以上文献为示例性质，实际研究中建议通过PubMed或Web of Science检索具体论文。
**Background of PCCa Recombinant Protein**
PCCa (Prostate Cancer Coagulase antigen) recombinant protein is a genetically engineered biomolecule derived from the coagulase gene expressed in prostate cancer cells. Initially identified for its association with prostate cancer progression, PCCa has garnered attention as a potential tumor-associated antigen due to its overexpression in malignant prostate tissues compared to normal or benign tissues. The recombinant form of PCCa is produced using prokaryotic or eukaryotic expression systems, enabling scalable and standardized production for research and therapeutic applications.
The interest in PCCa stems from its role in cancer immunology. Studies suggest that PCCa may participate in tumor immune evasion mechanisms, making it a target for immunotherapy. Recombinant PCCa protein serves as a critical tool in developing diagnostic assays, such as ELISA-based detection of anti-PCCa antibodies in patient sera, which could aid in early cancer detection or monitoring treatment responses. Additionally, it is explored as a component in therapeutic vaccines aimed at eliciting antigen-specific T-cell responses to combat prostate cancer.
Current research also focuses on PCCa's molecular interactions, particularly its potential involvement in coagulation pathways and tumor microenvironment modulation. These insights may uncover novel therapeutic strategies, such as combining PCCa-targeted therapies with immune checkpoint inhibitors. Despite promising preclinical data, clinical validation remains ongoing to establish its efficacy and safety in humans. Overall, PCCa recombinant protein represents a convergence point for advancing both diagnostic and immunotherapeutic innovations in oncology.
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