| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PLCb2 |
| Uniprot No | Q00722 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1185aa |
| 氨基酸序列 | MSLLNPVLLPPKVKAYLSQGERFIKWDDETTVASPVILRVDPKGYYLYWTYQSKEMEFLDITSIRDTRFGKFAKMPKSQKLRDVFNMDFPDNSFLLKTLTVVSGPDMVDLTFHNFVSYKENVGKAWAEDVLALVKHPLTANASRSTFLDKILVKLKMQLNSEGKIPVKNFFQMFPADRKRVEAALSACHLPKGKNDAINPEDFPEPVYKSFLMSLCPRPEIDEIFTSYHAKAKPYMTKEHLTKFINQKQRDSRLNSLLFPPARPDQVQGLIDKYEPSGINAQRGQLSPEGMVWFLCGPENSVLAQDKLLLHHDMTQPLNHYFINSSHNTYLTAGQFSGLSSAEMYRQVLLSGCRCVELDCWKGKPPDEEPIITHGFTMTTDIFFKEAIEAIAESAFKTSPYPIILSFENHVDSPRQQAKMAEYCRTIFGDMLLTEPLEKFPLKPGVPLPSPEDLRGKILIKNKKNQFSGPTSSSKDTGGEAEGSSPPSAPAGEGTVWAGEEGTELEEEEVEEEEEEESGNLDEEEIKKMQSDEGTAGLEVTAYEEMSSLVNYIQPTKFVSFEFSAQKNRSYVISSFTELKAYDLLSKASVQFVDYNKRQMSRIYPKGTRMDSSNYMPQMFWNAGCQMVALNFQTMDLPMQQNMAVFEFNGQSGYLLKHEFMRRPDKQFNPFSVDRIDVVVATTLSITVISGQFLSERSVRTYVEVELFGLPGDPKRRYRTKLSPSTNSINPVWKEEPFVFEKILMPELASLRVAVMEEGNKFLGHRIIPINALNSGYHHLCLHSESNMPLTMPALFIFLEMKDYIPGAWADLTVALANPIKFFSAHDTKSVKLKEAMGGLPEKPFPLASPVASQVNGALAPTSNGSPAARAGAREEAMKEAAEPRTASLEELRELKGVVKLQRRHEKELRELERRGARRWEELLQRGAAQLAELGPPGVGGVGACKLGPGKGSRKKRSLPREESAGAAPGEGPEGVDGRVRELKDRLELELLRQGEEQYECVLKRKEQHVAEQISKMMELAREKQAAELKALKETSENDTKEMKKKLETKRLERIQGMTKVTTDKMAQERLKREINNSHIQEVVQVIKQMTENLERHQEKLEEKQAACLEQIREMEKQFQKEALAEYEARMKGLEAEVKESVRACLRTCFPSEAKDKPERACECPPELCEQDPLIAKADAQESRL |
| 预测分子量 | 134 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PLCβ2重组蛋白的模拟参考文献示例(注：内容为模拟生成，建议通过学术数据库验证)：
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1. **文献名称**: *"Recombinant Expression and Functional Characterization of PLCβ2 in Insect Cells"*
**作者**: Smith A, et al.
**摘要**: 本研究利用杆状病毒-昆虫细胞系统成功表达了重组PLCβ2蛋白，并验证其磷酸肌醇水解活性。结果表明，PLCβ2的催化效率受Gαq亚基调控，为解析GPCR信号通路机制提供了工具。
2. **文献名称**: *"Structural Insights into PLCβ2 Activation by G-Protein Coupled Receptors"*
**作者**: Zhang Y, et al.
**摘要**: 通过冷冻电镜解析了重组PLCβ2与Gβγ复合体的高分辨率结构，揭示了其膜结合域与效应蛋白的相互作用界面，阐明了受体激活后的构象变化机制。
3. **文献名称**: *"PLCβ2 in Immune Cell Signaling: Role of Recombinant Protein in Neutrophil Chemotaxis"*
**作者**: Jones R, et al.
**摘要**: 利用大肠杆菌表达的重组PLCβ2蛋白，证实其在趋化因子诱导的中性粒细胞迁移中起关键作用，并通过体外实验证明PLCβ2缺失导致IP3生成减少及钙信号异常。
4. **文献名称**: *"High-Throughput Screening of PLCβ2 Inhibitors Using a Fluorescent-Based Assay"*
**作者**: Lee H, et al.
**摘要**: 开发基于重组PLCβ2蛋白的荧光底物检测法，筛选出新型小分子抑制剂，为治疗PLCβ2异常激活相关的炎症性疾病提供了先导化合物。
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建议通过PubMed、Google Scholar等平台以关键词“PLCβ2 recombinant”、“PLCbeta2 expression”检索最新文献以获取准确信息。
PLCβ2 (Phospholipase C beta 2) is a key enzyme in intracellular signaling pathways, primarily involved in hydrolyzing phosphatidylinositol 4.5-bisphosphate (PIP₂) into secondary messengers inositol 1.4.5-trisphosphate (IP₃) and diacylglycerol (DAG). As a member of the phospholipase C family, it is uniquely regulated by G-protein-coupled receptors (GPCRs) through interaction with Gβγ subunits, distinguishing it from other PLC isoforms activated by tyrosine kinases or small GTPases. PLCβ2 is predominantly expressed in hematopoietic cells, the brain, and sensory organs, playing critical roles in immune responses, neuronal signaling, and sensory transduction.
Recombinant PLCβ2 protein is engineered for research and therapeutic applications, allowing precise study of its structure-function relationships, activation mechanisms, and interaction partners. Its modular domains—including a pleckstrin homology (PH) domain, EF-hand motifs, and catalytic TIM barrel—are conserved in recombinant forms, enabling investigations into calcium signaling, membrane association, and downstream pathways. Dysregulation of PLCβ2 is implicated in immune disorders, cancer metastasis, and neuropathologies, making its recombinant version valuable for drug screening and molecular diagnostics.
Production typically involves expression systems like E. coli or mammalian cells, followed by affinity purification (e.g., His-tag). Researchers utilize recombinant PLCβ2 to dissect GPCR signaling crosstalk, develop inhibitors for overactive PLC pathways, or engineer biosensors for real-time IP₃/DAG monitoring. Its role in B-cell receptor signaling and platelet activation further underscores its relevance in immunology and hematology. Compared to PLCβ1 or PLCβ3. PLCβ2 exhibits distinct tissue distribution and activation kinetics, highlighting the importance of isoform-specific studies. Current challenges include maintaining post-translational modifications in vitro and resolving conformational dynamics during membrane binding.
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