| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RGS3 |
| Uniprot No | P49796 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1198aa |
| 氨基酸序列 | MPVIPALWEVEMGRSQGQEIETILANRSHSDSTPLPNFLSGSHRPECCTCRLLTASGAQDSLPFGRRLYSGPWRSCEEVCHVSVLSVLSTSCGLSLSLPIFPGWMEWLSPDIALPRRDEWTQTSPARKRITHAKVQGAGQLRLSIDAQDRVLLLHIIEGKGLISKQPGTCDPYVKISLIPEDSRLRHQKTQTVPDCRDPAFHEHFFFPVQEEDDQKRLLVTVWNRASQSRQSGLIGCMSFGVKSLLTPDKEISGWYYLLGEHLGRTKHLKVARRRLRPLRDPLLRMPGGGDTENGKKLKITIPRGKDGFGFTICCDSPVRVQAVDSGGPAERAGLQQLDTVLQLNERPVEHWKCVELAHEIRSCPSEIILLVWRMVPQVKPGPDGGVLRRASCKSTHDLQSPPNKREKNCTHGVQARPEQRHSCHLVCDSSDGLLLGGWERYTEVAKRGGQHTLPALSRATAPTDPNYIILAPLNPGSQLLRPVYQEDTIPEESGSPSKGKSYTGLGKKSRLMKTVQTMKGHGNYQNCPVVRPHATHSSYGTYVTLAPKVLVFPVFVQPLDLCNPARTLLLSEELLLYEGRNKAAEVTLFAYSDLLLFTKEDEPGRCDVLRNPLYLQSVKLQEGSSEDLKFCVLYLAEKAECLFTLEAHSQEQKKRVCWCLSENIAKQQQLAASPPDSKMFETEADEKREMALEEGKGPGAEDSPPSKEPSPGQELPPGQDLPPNKDSPSGQEPAPSQEPLSSKDSATSEGSPPGPDAPPSKDVPPCQEPPPAQDLSPCQDLPAGQEPLPHQDPLLTKDLPAIQESPTRDLPPCQDLPPSQVSLPAKALTEDTMSSGDLLAATGDPPAAPRPAFVIPEVRLDSTYSQKAGAEQGCSGDEEDAEEAEEVEEGEEGEEDEDEDTSDDNYGERSEAKRSSMIETGQGAEGGLSLRVQNSLRRRTHSEGSLLQEPRGPCFASDTTLHCSDGEGAASTWGMPSPSTLKKELGRNGGSMHHLSLFFTGHRKMSGADTVGDDDEASRKRKSKNLAKDMKNKLGIFRRRNESPGAPPAGKADKMMKSFKPTSEEALKWGESLEKLLVHKYGLAVFQAFLRTEFSEENLEFWLACEDFKKVKSQSKMASKAKKIFAEYIAIQACKEVNLDSYTREHTKDNLQSVTRGCFDLAQKRIFGLMEKDSYPRFLRSDLYLDLINQKKMSPPL |
| 预测分子量 | 132,3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RGS3重组蛋白的3篇参考文献，涵盖其功能、表达及作用机制：
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1. **"Regulators of G-protein signaling (RGS) proteins: new central nervous system drug targets?"**
*Hollinger S, Hepler JR*
*摘要*：综述了RGS蛋白家族(包括RGS3)在G蛋白信号通路中的调控作用，探讨其重组蛋白在药物开发中的潜力，特别关注其通过加速Gα亚基的GTP酶活性终止信号传递的机制。
2. **"RGS3 interacts with 14-3-3 via the N-terminal region distinct from the RGS domain"**
*Chen C, Zheng B, Han J, Lin SC*
*摘要*：利用重组RGS3蛋白研究其与14-3-3蛋白的相互作用，发现RGS3的N端区域介导此结合，揭示了其在细胞定位和信号调控中的新机制，可能与细胞增殖和凋亡相关。
3. **"Regulator of G-protein signaling 3 suppresses tumorigenesis in colorectal carcinoma"**
*Li H, He C, Wang J, et al.*
*摘要*：通过体外重组RGS3蛋白表达实验，证明其过表达抑制结直肠癌细胞增殖并诱导凋亡，机制涉及调控Wnt/β-catenin和MAPK通路，提示RGS3的肿瘤抑制作用。
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**注**：上述文献为示例，实际引用时请核实期刊、年份及具体内容。如需真实文献，建议通过PubMed或Google Scholar以关键词“RGS3 recombinant protein”或“RGS3 functional study”检索近期研究。
Regulator of G-protein signaling 3 (RGS3) is a member of the RGS protein family, which plays a critical role in modulating G-protein-coupled receptor (GPCR) signaling pathways. RGS proteins act as GTPase-activating proteins (GAPs) for Gα subunits of heterotrimeric G proteins, accelerating the hydrolysis of GTP to terminate signaling. RGS3. in particular, negatively regulates Gαq and Gαi/o subunits, influencing diverse cellular processes such as cell proliferation, migration, and immune responses. Dysregulation of RGS3 has been implicated in pathologies including cancer, cardiovascular diseases, and inflammatory disorders.
Recombinant RGS3 protein is engineered through molecular cloning and expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional protein for research. Its structure typically includes a conserved RGS domain for Gα interaction and variable N- or C-terminal regions that determine subcellular localization and partner protein binding. Alternative splicing generates isoforms like RGS3S (short) and RGS3L (long), each exhibiting distinct regulatory roles. Recombinant RGS3 enables mechanistic studies of GPCR signaling dynamics, screening for pharmacological agents targeting RGS-Gα interactions, and exploration of its disease-related functions. For instance, RGS3 overexpression has been linked to suppressed tumor growth in certain cancers, while its downregulation may contribute to hypertension. The protein’s post-translational modifications (e.g., phosphorylation) and tissue-specific expression further underscore its regulatory complexity. By providing a controlled, high-purity tool, recombinant RGS3 facilitates both *in vitro* and *in vivo* investigations into GPCR pathway modulation, offering potential therapeutic insights for disorders driven by aberrant G-protein signaling.
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