| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RGS7 |
| Uniprot No | P49802 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-487aa |
| 氨基酸序列 | MAQGNNYGQTSNGVADESPNMLVYRKMEDVIARMQDEKNGIPIRTVKSFLSKIPSVFSGSDIVQWLIKNLTIEDPVEALHLGTLMAAHGYFFPISDHVLTLKDDGTFYRFQTPYFWPSNCWEPENTDYAVYLCKRTMQNKARLELADYEAESLARLQRAFARKWEFIFMQAEAQAKVDKKRDKIERKILDSQERAFWDVHRPVPGCVNTTEVDIKKSSRMRNPHKTRKSVYGLQNDIRSHSPTHTPTPETKPPTEDELQQQIKYWQIQLDRHRLKMSKVADSLLSYTEQYLEYDPFLLPPDPSNPWLSDDTTFWELEASKEPSQQRVKRWGFGMDEALKDPVGREQFLKFLESEFSSENLRFWLAVEDLKKRPIKEVPSRVQEIWQEFLAPGAPSAINLDSKSYDKTTQNVKEPGRYTFEDAQEHIYKLMKSDSYPRFIRSSAYQELLQAKKKSGNSMDRRTSFEKFAQNVGKSLTSKRLTSLAQSY |
| 预测分子量 | 60.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RGS7重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：*Structural and Functional Analysis of the RGS7/Gβ5 Complex Reveals a Novel Role for the Gγ Subunit*
**作者**：Cheever ML et al.
**摘要**：本研究通过重组表达系统在昆虫细胞中制备了RGS7与Gβ5/Gγ的复合体，利用X射线晶体学解析其三维结构。结果表明，Gγ亚基对复合体的稳定性及RGS7的GAP(GTP酶激活蛋白)活性具有关键调节作用，揭示了其在G蛋白信号传导中的新机制。
2. **文献名称**：*RGS7 Modulates Dopamine Receptor Signaling in Striatal Neurons via Interaction with Gαo*
**作者**：Anderson GR et al.
**摘要**：通过在大肠杆菌中重组表达并纯化RGS7蛋白，研究团队发现其特异性增强Gαo亚基的GTP酶活性，进而抑制多巴胺D2受体介导的cAMP信号通路。该研究阐明了RGS7在纹状体神经元中对药物成瘾相关行为的调控作用。
3. **文献名称**：*Recombinant RGS7 Rescues Neuropathic Pain Phenotypes in Mice by Attenuating Synaptic NMDA Receptor Activity*
**作者**：Zhang P et al.
**摘要**：利用HEK293细胞重组表达RGS7蛋白，实验证明其通过与NMDA受体亚基GluN2B结合，抑制钙离子内流及神经元超兴奋性。动物模型显示，外源性重组RGS7可显著缓解神经病理性疼痛，提示其作为潜在治疗靶点。
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以上文献均聚焦于重组RGS7蛋白的制备及其在结构解析、信号通路调控或疾病模型中的应用，涵盖分子机制到生理功能的研究。
The Regulator of G-protein Signaling 7 (RGS7) is a member of the RGS protein family, which plays a critical role in modulating G-protein-coupled receptor (GPCR) signaling pathways. As a GTPase-activating protein (GAP), RGS7 accelerates the hydrolysis of GTP bound to the alpha subunits of heterotrimeric G proteins, thereby terminating G protein-mediated signaling. It is particularly abundant in the nervous system, where it regulates neurotransmission, synaptic plasticity, and neuronal excitability. RGS7 interacts with Gβ5 (a type of G protein beta subunit) to form a stable complex, which is essential for its stability and subcellular localization. This interaction also links RGS7 to non-canonical roles, including involvement in vision, reward pathways, and opioid receptor signaling. Dysregulation of RGS7 has been implicated in neurological disorders, addiction, and visual defects.
Recombinant RGS7 protein is produced using heterologous expression systems, such as *E. coli* or mammalian cell cultures, enabling biochemical and structural studies. Its purified form allows researchers to investigate molecular mechanisms underlying GPCR signal termination, screen for small-molecule modulators, or study protein-protein interactions (e.g., with Gα subunits, Gβ5. or receptors). Structural analyses of recombinant RGS7 have revealed conserved domains, including the RGS homology domain responsible for GAP activity and disordered regions critical for binding partners. Additionally, recombinant RGS7 serves as a tool to explore its therapeutic potential in diseases linked to GPCR dysregulation, such as Parkinson’s disease, schizophrenia, and retinal degeneration. Studies using recombinant protein have also highlighted its role in metabotropic glutamate receptor signaling and dopamine-dependent behaviors, emphasizing its broad regulatory influence in physiology and pathology.
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