| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PLCd1 |
| Uniprot No | P51178 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-756aa |
| 氨基酸序列 | MDSGRDFLTL HGLQDDEDLQ ALLKGSQLLK VKSSSWRRER FYKLQEDCKT IWQESRKVMR TPESQLFSIE DIQEVRMGHR TEGLEKFARD VPEDRCFSIV FKDQRNTLDL IAPSPADAQH WVLGLHKIIH HSGSMDQRQK LQHWIHSCLR KADKNKDNKM SFKELQNFLK ELNIQVDDSY ARKIFRECDH SQTDSLEDEE IEAFYKMLTQ RVEIDRTFAE AAGSGETLSV DQLVTFLQHQ QREEAAGPAL ALSLIERYEP SETAKAQRQM TKDGFLMYLL SADGSAFSLA HRRVYQDMGQ PLSHYLVSSS HNTYLLEDQL AGPSSTEAYI RALCKGCRCL ELDCWDGPNQ EPIIYHGYTF TSKILFCDVL RAIRDYAFKA SPYPVILSLE NHCTLEQQRV MARHLHAILG PMLLNRPLDG VTNSLPSPEQ LKGKILLKGK KLGGLLPPGG EGGPEATVVS DEDEAAEMED EAVRSRVQHK PKEDKLRLAQ ELSDMVIYCK SVHFGGFSSP GTPGQAFYEM ASFSENRALR LLQESGNGFV RHNVGHLSRI YPAGWRTDSS NYSPVEMWNG GCQIVALNFQ TPGPEMDVYQ GRFQDNGACG YVLKPAFLRD PNGTFNPRAL AQGPWWARKR LNIRVISGQQ LPKVNKNKNS IVDPKVTVEI HGVSRDVASR QTAVITNNGF NPWWDTEFAF EVVVPDLALI RFLVEDYDAS SKNDFIGQST IPLNSLKQGY RHVHLMSKNG DQHPSATLFV KISLQD |
| 预测分子量 | 85,6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PLCδ1重组蛋白的3篇示例参考文献(注：文献为虚构示例，实际文献需通过学术数据库检索)：
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1. **文献名称**：*"Functional characterization of recombinant PLCδ1 expressed in Sf9 insect cells"*
**作者**：Tanaka K, et al.
**摘要**：本研究利用杆状病毒系统在昆虫细胞中高效表达重组人PLCδ1蛋白，纯化后验证其水解PIP2的酶活性，并发现其催化功能依赖Ca²⁺浓度，为体外信号通路研究提供工具。
2. **文献名称**：*"Crystal structure of PLCδ1 PH domain and its role in membrane binding"*
**作者**：Ferguson KM, et al.
**摘要**：解析了PLCδ1的PH结构域晶体结构，揭示其通过特异性结合PIP2锚定细胞膜的机制，为设计靶向该结构域的抑制剂奠定基础。
3. **文献名称**：*"Recombinant PLCδ1 modulates ERK signaling in hepatocellular carcinoma cells"*
**作者**：Zhang R, et al.
**摘要**：通过外源添加重组PLCδ1蛋白，证明其通过IP3/DAG通路抑制肝癌细胞ERK磷酸化，提示其在肿瘤治疗中的潜在应用价值。
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**建议**：实际研究中，可通过PubMed或Google Scholar搜索关键词“PLCδ1 recombinant protein”、“phospholipase C delta 1 purification”或“PLCδ1 structure-function”获取最新文献。经典研究可参考：
- **真实文献示例**：*"Structure of the high affinity complex of inositol trisphosphate with a phospholipase C pleckstrin homology domain"* (Ferguson KM, et al., *Cell*, 1995) 分析了PH结构域与IP3的相互作用。
**Background of PLCδ1 Recombinant Protein**
Phospholipase C delta 1 (PLCδ1) is a critical enzyme in intracellular signaling, primarily involved in hydrolyzing phosphatidylinositol 4.5-bisphosphate (PIP₂) into secondary messengers inositol 1.4.5-trisphosphate (IP₃) and diacylglycerol (DAG). These molecules regulate calcium release from endoplasmic reticulum and protein kinase C (PKC) activation, influencing processes like cell proliferation, differentiation, and apoptosis. PLCδ1. a member of the PLC family, is characterized by its modular domains, including a pleckstrin homology (PH) domain, EF-hand motifs, and catalytic X/Y regions, which enable lipid binding, calcium sensitivity, and enzymatic activity.
Recombinant PLCδ1 protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to produce high-purity, functional enzyme for research and therapeutic applications. Its recombinant form retains native structural and functional properties, making it valuable for studying PLC-mediated signaling cascades, particularly in diseases linked to dysregulated phosphoinositide pathways, such as cancer, cardiovascular disorders, and neurological conditions.
PLCδ1’s role as a calcium-sensitive PLC isoform has also spurred interest in drug discovery, where it serves as a target or tool for modulating cellular responses. Additionally, recombinant PLCδ1 is utilized in diagnostic assays, structural studies, and screening small molecules to identify pathway modulators. Despite its lower abundance compared to other PLC isoforms, PLCδ1’s unique regulatory mechanisms and tissue-specific expression underscore its biological significance. Ongoing research aims to clarify its precise roles in health and disease, leveraging recombinant protein technology to advance mechanistic and translational insights.
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