| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EIF2C1 |
| Uniprot No | Q9UL18 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-857aa |
| 氨基酸序列 | MEAGPSGAAAGAYLPPLQQVFQAPRRPGIGTVGKPIKLLANYFEVDIPKIDVYHYEVDIKPDKCPRRVNREVVEYMVQHFKPQIFGDRKPVYDGKKNIYTVTALPIGNERVDFEVTIPGEGKDRIFKVSIKWLAIVSWRMLHEALVSGQIPVPLESVQALDVAMRHLASMRYTPVGRSFFSPPEGYYHPLGGGREVWFGFHQSVRPAMWKMMLNIDVSATAFYKAQPVIEFMCEVLDIRNIDEQPKPLTDSQRVRFTKEIKGLKVEVTHCGQMKRKYRVCNVTRRPASHQTFPLQLESGQTVECTVAQYFKQKYNLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAGQRCIKKLTDNQTSTMIKATARSAPDRQEEISRLMKNASYNLDPYIQEFGIKVKDDMTEVTGRVLPAPILQYGGRNRAIATPNQGVWDMRGKQFYNGIEIKVWAIACFAPQKQCREEVLKNFTDQLRKISKDAGMPIQGQPCFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTSPQTLSNLCLKINVKLGGINNILVPHQRSAVFQQPVIFLGADVTHPPAGDGKKPSITAVVGSMDAHPSRYCATVRVQRPRQEIIEDLSYMVRELLIQFYKSTRFKPTRIIFYRDGVPEGQLPQILHYELLAIRDACIKLEKDYQPGITYIVVQKRHHTRLFCADKNERIGKSGNIPAGTTVDTNITHPFEFDFYLCSHAGIQGTSRPSHYYVLWDDNRFTADELQILTYQLCHTYVRCTRSVSIPAPAYYARLVAFRARYHLVDKEHDSGEGSHISGQSNGRDPQALAKAVQVHQDTLRTMYFA |
| 预测分子量 | 97,2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于EIF2C1重组蛋白的3篇模拟参考文献(注：部分文献信息为示例性概括，实际文献需通过数据库验证)：
---
1. **文献名称**: *Structural and functional analysis of recombinant human EIF2C1 in RNA-induced silencing complex*
**作者**: Meister G., et al.
**摘要**: 本研究通过重组表达技术纯化了人源EIF2C1蛋白，并解析其与siRNA结合的晶体结构。实验表明，EIF2C1通过PAZ结构域特异性识别RNA的3'端，在RNA诱导沉默复合体(RISC)组装中起关键作用。
2. **文献名称**: *Recombinant EIF2C1 expression and its role in microRNA-mediated gene regulation*
**作者**: Parker R., et al.
**摘要**: 通过大肠杆菌系统表达并纯化重组EIF2C1蛋白，研究其在microRNA通路中的功能。结果显示，EIF2C1通过结合Argonaute家族成员参与靶mRNA的翻译抑制，并依赖其PIWI结构域的核酸内切酶活性。
3. **文献名称**: *Engineering of EIF2C1 recombinant protein for enhanced stability in gene therapy applications*
**作者**: Zeng Y., et al.
**摘要**: 报道了一种优化的重组EIF2C1蛋白表达体系，通过引入热稳定性突变体提高了蛋白在体外基因沉默实验中的活性，为基于RNA干扰的基因治疗提供了潜在工具。
---
如需实际文献，建议通过PubMed或Google Scholar检索关键词“EIF2C1 recombinant protein”或“Argonaute1 recombinant expression”获取具体文章。
**Background of EIF2C1 Recombinant Protein**
EIF2C1. also known as Argonaute 1 (AGO1), is a member of the Argonaute protein family, which plays a central role in RNA-mediated gene silencing pathways. This protein is critical for the RNA-induced silencing complex (RISC), where it binds small non-coding RNAs, such as microRNAs (miRNAs) and small interfering RNAs (siRNAs), to regulate gene expression post-transcriptionally. By guiding RISC to complementary mRNA targets, EIF2C1 facilitates mRNA degradation or translational repression, influencing cellular processes like development, differentiation, and apoptosis.
The recombinant EIF2C1 protein is engineered through molecular cloning techniques, typically expressed in *E. coli* or mammalian systems, to ensure high purity and functional activity. Recombinant versions allow researchers to study its structure, interactions, and mechanisms in vitro, bypassing the complexity of isolating it from native tissues. Structural studies reveal that EIF2C1 contains conserved PAZ and PIWI domains, which are essential for RNA binding and endonuclease activity, respectively.
Research on EIF2C1 has implications in understanding diseases linked to dysregulated gene expression, including cancer, neurodegenerative disorders, and viral infections. For example, aberrant miRNA expression mediated by EIF2C1 is associated with tumor progression, making it a potential therapeutic target. Additionally, recombinant EIF2C1 is utilized in biotechnology for developing RNA interference (RNAi) tools and gene-editing applications.
Despite its significance, challenges remain in characterizing its precise regulatory networks and post-translational modifications. Ongoing studies aim to elucidate its role in cellular stress responses and its interplay with other Argonaute family members. The availability of recombinant EIF2C1 continues to accelerate both basic research and translational applications in molecular biology.
×
