| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | C22orf28 |
| Uniprot No | Q9Y3I0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-505aa |
| 氨基酸序列 | MSRSYNDELQ FLEKINKNCW RIKKGFVPNM QVEGVFYVND ALEKLMFEEL RNACRGGGVG GFLPAMKQIG NVAALPGIVH RSIGLPDVHS GYGFAIGNMA AFDMNDPEAV VSPGGVGFDI NCGVRLLRTN LDESDVQPVK EQLAQAMFDH IPVGVGSKGV IPMNAKDLEE ALEMGVDWSL REGYAWAEDK EHCEEYGRML QADPNKVSAR AKKRGLPQLG TLGAGNHYAE IQVVDEIFNE YAAKKMGIDH KGQVCVMIHS GSRGLGHQVA TDALVAMEKA MKRDKIIVND RQLACARIAS PEGQDYLKGM AAAGNYAWVN RSSMTFLTRQ AFAKVFNTTP DDLDLHVIYD VSHNIAKVEQ HVVDGKERTL LVHRKGSTRA FPPHHPLIAV DYQLTGQPVL IGGTMGTCSY VLTGTEQGMT ETFGTTCHGA GRALSRAKSR RNLDFQDVLD KLADMGIAIR VASPKLVMEE APESYKNVTD VVNTCHDAGI SKKAIKLRPI AVIKG |
| 预测分子量 | 55,2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于C22orf28重组蛋白的假设性参考文献示例(仅供参考，实际文献需通过学术数据库查询)：
1. **标题**：*Cloning and Expression of Recombinant C22orf28 Protein in Escherichia coli*
**作者**：Smith A, et al.
**摘要**：该研究成功在大肠杆菌中克隆并表达了C22orf28重组蛋白，优化了表达条件并通过亲和层析纯化。初步功能实验表明，该蛋白可能参与细胞周期调控。
2. **标题**：*Functional Characterization of C22orf28 Using Recombinant Protein Technology*
**作者**：Johnson B, et al.
**摘要**：通过哺乳动物细胞表达系统制备C22orf28重组蛋白，发现其与MAPK信号通路中的激酶相互作用，提示其在细胞应激反应中的潜在作用。
3. **标题**：*Structural Analysis of Recombinant C22orf28 and Its Putative Role in DNA Repair*
**作者**：Lee C, et al.
**摘要**：利用重组表达的C22orf28蛋白进行X射线晶体学分析，鉴定出N端DNA结合结构域，并发现其与DNA损伤修复相关蛋白存在共定位现象。
4. **标题**：*C22orf28 Recombinant Protein as a Potential Biomarker in Cancer Studies*
**作者**：Wang D, et al.
**摘要**：通过重组蛋白制备抗体，检测多种肿瘤组织中C22orf28的表达水平，发现其高表达与乳腺癌患者预后不良显著相关。
**注**：以上为模拟文献，实际研究中请通过PubMed、Web of Science等平台检索最新成果。若C22orf28功能尚不明确，建议扩大关键词范围(如结合染色体22相关疾病或通路)。
**Background of C22orf28 Recombinant Protein**
C22orf28 (Chromosome 22 Open Reading Frame 28) is a protein-coding gene located on human chromosome 22. While its biological functions remain poorly characterized, emerging studies suggest potential roles in cellular processes such as transcriptional regulation, protein interactions, or stress responses. The gene is evolutionarily conserved across vertebrates, implying functional importance, but its specific mechanisms and pathways are not yet fully elucidated.
The C22orf28 protein is predicted to contain intrinsically disordered regions, which may facilitate dynamic interactions with other biomolecules. Its expression has been detected in various tissues, including the brain, testis, and immune cells, though at relatively low levels. Dysregulation of C22orf28 has been tentatively linked to diseases such as gliomas, Alzheimer’s disease, and certain cancers, though evidence remains preliminary. For example, altered expression in tumor tissues suggests a possible, yet unconfirmed, role in oncogenesis or tumor suppression.
Recombinant C22orf28 protein is engineered in vitro using expression systems (e.g., bacterial, mammalian) to enable functional studies. Its production allows researchers to investigate protein structure, post-translational modifications, binding partners, and cellular localization. Antibodies generated against recombinant C22orf28 aid in detecting endogenous protein levels, facilitating disease biomarker research.
Current research focuses on clarifying its molecular interactions, such as potential involvement in RNA processing or chromatin remodeling, based on sequence motifs and domain predictions. However, the lack of comprehensive in vivo data highlights the need for further exploration. Recombinant C22orf28 serves as a critical tool to bridge this knowledge gap, offering insights into its physiological and pathological significance.
In summary, C22orf28 represents an understudied gene with emerging relevance in human health, and recombinant protein technology remains pivotal in unraveling its biological and clinical implications.
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