| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ENTK |
| Uniprot No | P98073 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1019aa |
| 氨基酸序列 | MGSKRGISSRHHSLSSYEIMFAALFAILVVLCAGLIAVSCLTIKESQRGAALGQSHEARATFKITSGVTYNPNLQDKLSVDFKVLAFDLQQMIDEIFLSSNLKNEYKNSRVLQFENGSIIVVFDLFFAQWVSDENVKEELIQGLEANKSSQLVTFHIDLNSVDILDKLTTTSHLATPGNVSIECLPGSSPCTDALTCIKADLFCDGEVNCPDGSDEDNKMCATVCDGRFLLTGSSGSFQATHYPKPSETSVVCQWIIRVNQGLSIKLSFDDFNTYYTDILDIYEGVGSSKILRASIWETNPGTIRIFSNQVTATFLIESDESDYVGFNATYTAFNSSELNNYEKINCNFEDGFCFWVQDLNDDNEWERIQGSTFSPFTGPNFDHTFGNASGFYISTPTGPGGRQERVGLLSLPLDPTLEPACLSFWYHMYGENVHKLSINISNDQNMEKTVFQKEGNYGDNWNYGQVTLNETVKFKVAFNAFKNKILSDIALDDISLTYGICNGSLYPEPTLVPTPPPELPTDCGGPFELWEPNTTFSSTNFPNSYPNLAFCVWILNAQKGKNIQLHFQEFDLENINDVVEIRDGEEADSLLLAVYTGPGPVKDVFSTTNRMTVLLITNDVLARGGFKANFTTGYHLGIPEPCKADHFQCKNGECVPLVNLCDGHLHCEDGSDEADCVRFFNGTTNNNGLVRFRIQSIWHTACAENWTTQISNDVCQLLGLGSGNSSKPIFPTDGGPFVKLNTAPDGHLILTPSQQCLQDSLIRLQCNHKSCGKKLAAQDITPKIVGGSNAKEGAWPWVVGLYYGGRLLCGASLVSSDWLVSAAHCVYGRNLEPSKWTAILGLHMKSNLTSPQTVPRLIDEIVINPHYNRRRKDNDIAMMHLEFKVNYTDYIQPICLPEENQVFPPGRNCSIAGWGTVVYQGTTANILQEADVPLLSNERCQQQMPEYNITENMICAGYEEGGIDSCQGDSGGPLMCQENNRWFLAGVTSFGYKCALPNRPGVYARVSRFTEWIQSFLH |
| 预测分子量 | 112,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于ENTK重组蛋白的3篇参考文献示例，涵盖其重组表达及功能研究：
1. **文献名称**：*Functional analysis of the biosynthetic transport protein EntK in nukacin ISK-1 production*
**作者**：Sashihara, T., et al.
**摘要**：研究阐明了ENTK作为细菌素nukacin ISK-1输出系统的关键膜蛋白，通过重组表达验证其跨膜转运功能，并证明其缺失导致抗菌活性丧失。
2. **文献名称**：*Reconstitution of the EntK-mediated transport system in artificial lipid bilayers*
**作者**：van den Berg van Saparoea, L.B., et al.
**摘要**：利用重组ENTK蛋白在脂质体重建其转运体系，证实其依赖ATP水解的底物跨膜运输机制，为膜蛋白功能研究提供模型。
3. **文献名称**：*Crystal structure and molecular dynamics of the EntK ABC transporter from Streptomyces*
**作者**：Yamada, S., et al.
**摘要**：通过X射线晶体学解析ENTK重组蛋白的三维结构，结合分子动力学模拟揭示其底物结合口袋构象变化及转运循环机制。
(注：以上文献为示例性内容，实际引用时请核对真实文献。)
ENTK recombinant protein is a biotechnologically engineered derivative of the EntK enzyme, originally identified in certain bacterial species as a key component in cellular processes such as cell wall biosynthesis or stress response pathways. The name "ENTK" typically refers to its enzymatic function, often linked to kinase or transferase activity, though its precise biological role may vary depending on the organism of origin. For instance, in *Staphylococcus aureus*, EntK homologs are implicated in peptidoglycan remodeling, a critical process for bacterial cell division and virulence. This connection has drawn attention to ENTK as a potential target for novel antibacterial agents.
Recombinant ENTK is produced via heterologous expression systems, such as *E. coli* or yeast, enabling scalable production for research and industrial applications. The protein is purified using affinity chromatography, often incorporating tags like His-tags for efficient isolation. Structural studies of recombinant ENTK, including X-ray crystallography and cryo-EM, have elucidated its active sites and substrate-binding mechanisms, facilitating rational drug design.
In biotechnology, ENTK serves as a tool for studying bacterial resistance mechanisms, particularly in pathogens resistant to β-lactam antibiotics. It also finds utility in enzymology assays and synthetic biology for modifying biomolecules. Recent efforts focus on engineering ENTK variants with enhanced stability or altered substrate specificity, aiming to expand its applications in biocatalysis or therapeutic development. Despite progress, challenges remain in optimizing its activity under industrial conditions and minimizing immunogenicity for clinical use. Ongoing research aims to bridge these gaps, positioning ENTK as a versatile candidate for both academic and commercial endeavors.
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